Development of Antimicrobial Plant Extracts and its Application to Cosmetics

Abstract

This study is focused on finding new natural materials that have antimicrobial activity. We found that 3 plants extracts, Magnolia sieboldii K. Koch (M. sieboldii K. Koch), Rhus chinensis (R. chinensis) and Metasequioa glyptostroboides (M. glyptostroboides), have antimicrobial activities by disk diffusion method. We determined the Minimum Inhibitory Concentation (MIC) of each and found that 0.3 ∼ 0.35 % of M. glyptostroboides essential oil and 0.35 ∼ 0.4 % of M. sieboldii K. Koch extracts inhibited fungal growth and 0.45 ∼ 0.5 % of R. chinensis extracts inhibited bacterial growth. We isolated compounds from extracts and verified what have antimicrobial activity. As a result we found that caryophyllene oxide and caryophyllene isolated from M. glyptostroboides, costunolide and dehydrocostus lactone from M. sieboldii K. Koch and ethyl gallate, ethyl-3-gallate from R. chinensis have antimicrobial activities. In accordance with antimicrobial activity, O/W cosmetic emulsion containing mixture of 3 plants extracts showed preservative efficacy against both bacteria and fungi. Based on the above data we suggest that extracts from M. siebodii K. Koch, R. chinensis, and M. glyptostroboides, replace chemical synthetic preservatives and be applied as a natural preservative.

Enzymatic Synthesis of 1, 2-Hexanediol Galactoside by Whole Cells of β-Galactosidase-containing Recombinant Escherichia coli

“…최근 화장품 산업에서 첨가물 중의 하나인 방부제(살균/보 존제) 에 대한 부작용 문제가 대두 되고 있으며 [14,15], 그러한 이유로 방부제가 첨가되지 않은 화장품들에 대한 관심이 높아 지고 있고, 방부제에 대한 대체물 연구도 활발히 이루어지고 있다 [3,10]. 본 연구팀에서도 화장품에 사용하는 방부제인 chlorphenesin (CPN) [11]과 phenoxyethanol (PE) [7] (Fig.…”

Antimicrobial Activity of Essential Oils and Single Fragrance Ingredients for Cosmetic Applications

“…화장품은 다량의 물 탄소 및 질소를 공급하는 영양소 등이 포함되 어 있기 때문에 미생물 오염에 취약하다. 화장품 제조 과정 중 발생 하는 1차 오염과 소비자가 사용 중 발생할 수 있는 2차 오염이 존재 하며 장기간 사용 중 화장품 제형의 변질과 미생물 오염을 방지하기 위해 보존제가 필요하다 (Lee et al, 2012). 화장품에 사용되는 합성 보존제는 피부 자극 및 알레르기를 유발하여 부작용이 나타나 사용이 지양되고 있다 (Kim et al, 2006a).…”

Synergistic Antimicrobial Effect of Lonicera japonica and Magnolia obovata Extracts and Potential as a Plant-Derived Natural Preservative

“…However, recent studies have reported that preservatives, especially parabens, may have side effects. Consequently, the demand for plant-derived natural cosmetics has increased, thereby spurring developments in studies on plant-derived preservatives [7,8].…”