Development of Antibodies against <i>Aeromonas Salmonicida</i> in Trout

Krantz, George E.; Reddecliff, Jack M.

doi:10.4049/jimmunol.91.6.757

Cited by 47 publications

(4 citation statements)

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“…They are, however, still elevated compared to the non-vaccinated controls. Krantz et al showed elevated antibody levels in brown trout after an oil adjuvanted vaccination, sustaining the increased levels for at least 24 months [8]. Bricknell et al showed elevated antibody levels after un-adjuvanted vaccination of salmon, lasting at least 9 months [11].…”

Section: Discussionmentioning

confidence: 99%

“…Several studies on the effects of vaccination have been performed on salmon, possibly due to their economic role in modern aquaculture. However, some works have focused on rainbow trout, from the initial attempts on vaccination made by Duff in the 1940's [3], over later immunization studies by Krantz et al [8], and on to the work of Hastings, Stapleton and Ellis on the antibody responses towards extracellular products of A. salmonicida [12,13]. This study was initiated to provide a collected view of the role of antibodies in the induction of protection induced by the dominant oil adjuvanted i.p.…”

Section: Discussionmentioning

confidence: 99%

“…injected vaccines against A. salmonicida. Early on, studies using injected vaccines in brook trout and brown trout reported long-lasting, effective protection when using a mineral oil adjuvant [8]. Later studies performed in Atlantic salmon have shown similar results.…”

Section: Introductionmentioning

confidence: 90%

“…Immediately prior to administration, the bacterin was thoroughly mixed 1:1 with Freund's incomplete adjuvant (Sigma-Aldrich-F5506). An injection dose of a total of 50 ml experimental vaccine per fish therefore contained 1610 8 CFU.…”

Section: Experimental Auto Vaccinementioning

confidence: 99%

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Correction: Potential Role of Specific Antibodies as Important Vaccine Induced Protective Mechanism against Aeromonas salmonicida in Rainbow Trout

Villumsen¹,

Dalsgaard²,

Holten-Andersen³

et al. 2012

PLoS ONE

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Furunculosis caused by infection with Aeromonas salmonicida subsp. salmonicida has been a known threat to aquaculture for more than a century. Efficient prophylactic approaches against this disease are essential for continued growth of salmonid aquaculture. Since the introduction of successful oil-adjuvanted vaccines in the early 1990's, a number of studies have been published on the protective as well as adverse effects of these vaccines. Most studies focus on vaccination of salmon (Salmo salar). However, rainbow trout (Oncorhynchus mykiss) are also very susceptible to infection and are vaccinated accordingly. In this study we have examined the protection against infection with a Danish strain of A. salmonicida in both vaccinated and non-vaccinated rainbow trout. A commercial and an experimental auto-vaccine were tested. The protective effects of the vaccines were evaluated through an A. salmonicida challenge 18 weeks post vaccination. Both vaccines resulted in a significantly increased survival in the vaccinated fish during a 28 day challenge period relative to non-vaccinated fish (P = 0.01 and P = 0.001 for the commercial and experimental vaccine, respectively). Throughout the entire experiment, the presence of specific antibodies in plasma was monitored using ELISA. A significant increase in specific antibody levels was seen in fish vaccinated with both vaccines during the 18 weeks between vaccination and challenge. Within 3 days post challenge, a significant decrease in specific antibodies occurred in vaccinated fish. A positive correlation was found between mean levels of specific antibodies pre challenge and overall survival. This correlation, along with the observed depletion of antibodies during the initial phase of infection, suggests that specific antibodies play an essential role in vaccine mediated protection against A. salmonicida in rainbow trout.

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Section: Discussionmentioning

confidence: 99%