Development of antibodies against hydroxyatrazine and hydroxysimazine: Application to environmental samples

Lucas, Anne D.; Bekheit, Hassan K. M.; Goodrow, Marvin H.; Jones, A. Daniel; Kullman, Seth W.; Matsumura, Fumio; Woodrow, James E.; Seiber, James N.; Hammock, Bruce D.

doi:10.1021/jf00033a032

Cited by 27 publications

(11 citation statements)

References 16 publications

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“…The polyclonal antibodies 194, 841 and 842 were produced by Harrison et al [9]. The polyclonal antibody 2652 was produced by Lucas [18], the polyclonal 4653 is described in [30]. Five of the triazine herbicide derivatives were synthesized by Goodrow et al [31], one by Muldoon et al [20].…”

Section: Methodsmentioning

confidence: 99%

“…N-monodealkylated triazines, to a lower extent also didealkylated ones [18]. The last two antibodies are almost specific, so that their incorporation should largely facilitate the pattern recognition process and improve the cluster analysis.…”

Section: Analytesmentioning

confidence: 99%

“…Many groups have developed immunoassays for triazine herbicides such as atrazine [9][10][11][12], cyanazine [13], terbutylazine [14], terbutryn [15], hydroxyatrazine [16,17], or N-dealkylated triazines [18][19][20]. Cross reactivity to any other triazine herbicide besides the one an antibody has been developed for is and calibration curves involved.…”

Section: Introductionmentioning

confidence: 99%

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An approach to the construction of an immunoarray for differentiating and quantitating cross reacting analytes

Wortberg¹,

Jones²,

Kreissig³

et al. 1996

Analytica Chimica Acta

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We demonstrate the construction of an immunoarray to categorize, identify and quantitate different triazine herbicides or their environmental metabolites in water. In a first step cluster analysis is used to examine the performance of a small subset of antibodies out of a larger library, and hence to select a small array that is capable of categorizing the triazine herbicides into different groups. At the 1 ppb level it is possible to categorize an analyte as a chloro-s-triazine, a hydroxy metabolite, or as a methoxy /methylthio substituted triazine when using only 2-4 antibodies. At higher concentrations even the identity of the triazine can be determined with the same number of antibodies. A selected combination of antibodies is then used to identify and quantify Unknowns by comparing theii~unoassay responses to an array of calibration curves and usin~the maximum likelihood criterion.

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Section: Methodsmentioning

confidence: 99%

Section: Analytesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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An approach to the construction of an immunoarray for differentiating and quantitating cross reacting analytes

Wortberg¹,

Jones²,

Kreissig³

et al. 1996

Analytica Chimica Acta

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“…High affinity polyclonal and monoclonal antibodies were obtained from a series of logically designed striazine haptens. With these antibodies, useful assays have been developed for several parent s-triazines and some important metabolites (12)(13)(14)(15)(16)(17). The lowest limit of detection that has been achieved for one of the atrazine assays is 30 pg/ml (17).…”

Section: Pesticide Immunoassaysmentioning

confidence: 99%

Recent Developments in Immunoassays and Related Methods for the Detection of Xenobiotics

Wengatz

Harris

Gilman

et al. 1996

ACS Symposium Series

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“…Immunoassays meet these criteria. An increasing number of immunoassays that detect and quantify drugs and pesticides have been reported (Bekheit et al, 1993a(Bekheit et al, , 1993b; Goodrow et al, 1990;Karu et al, 1994;Kramer, et al, 1994;Lucas et al, 1993;Marco et al, 1993;Schneider et al, 1994;Vanderlaan et al, 1991). In this paper we describe a CI-ELISA useful for detecting ceftiofur sodium.…”

Section: Introductionmentioning

confidence: 99%

Competitive Indirect ELISA for Ceftiofur Sodium and the Effect of Different Immunizing and Coating Antigen Conjugates

Rose¹,

Kamps‐Holtzapple²,

Stanker³

1995

Bioconjugate Chem.

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Ceftiofur sodium is a broad spectrum, beta-lactamase-resistant cephalosporin. Ceftiofur and desfuroylceftiofur were used to develop competitive indirect enzyme-linked immunosorbent assays (CI-ELISA) for the determination of ceftiofur sodium. Hapten-protein conjugates were made using three different carrier proteins and three methods of conjugation. The first two methods use the free amine of ceftiofur as the site of conjugation, and coupling to bovine serum albumin and ovalbumin was achieved by using two different cross-linking reagents. The third conjugation procedure joins the hydrolyzed form of ceftiofur, desfuroylceftiofur, to the maleimide-activated carrier proteins, bovine serum albumin and keyhole limpet hemocyanin. A variety of immunization schedules is presented to show the effect of repeated immunizations on antibody maturation. Serum antibody levels were evaluated for each conjugation method using both homologous and heterologous conjugates as antigens. All of the immunogens resulted in the generation of anticeftiofur antibodies. The heterologous assay systems on average yielded more sensitive assays, but antisera obtained from all three immunogens were used successfully in developing enzyme-linked immunosorbent assays (ELISA's) for ceftiofur. Ceftiofur was detected in mouse sera in a concentration range of 3-500 ppb. The results illustrate that the method used to couple the hapten to a carrier protein as well as the site of coupling significantly influence the resulting enzyme-linked immunosorbent assays (ELISA's).

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Development of antibodies against hydroxyatrazine and hydroxysimazine: Application to environmental samples

Cited by 27 publications

References 16 publications

An approach to the construction of an immunoarray for differentiating and quantitating cross reacting analytes

An approach to the construction of an immunoarray for differentiating and quantitating cross reacting analytes

Recent Developments in Immunoassays and Related Methods for the Detection of Xenobiotics

Competitive Indirect ELISA for Ceftiofur Sodium and the Effect of Different Immunizing and Coating Antigen Conjugates

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