Abstract:The constant need for increased sensitivity of antibody‐based assays has increased the need for high affinity molecules. Use of Fab antibody fragments can increase the specificity and sensitivity of immunoassays. We developed simple GPC, SDS‐PAGE and IEF methods that can be used to characterize Fab material produced by reduction of F(ab’)2 fragments. Specific reduction of F(ab’)2 to Fab was accomplished using cysteamine HCl. The resulting Fab fragments were analyzed with and without alkylation with iodoacetami… Show more
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