Development of an Ultralow-Light-Level Luminescence Image Analysis System for Dynamic Measurements of Transcriptional Activity in Living and Migrating Cells

Maire, Éric; Lelièvre, Etienne; Brau, Daniel; Lyons, Andrew; Woodward, Michael P.; Fafeur, Véronique; Vandenbunder, Bernard

doi:10.1006/abio.2000.4503

Cited by 11 publications

(8 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is considered as an adaptive threshold because the resulting image is an image without local background levels. This function is particularly used in order to extract light intensity of peaks in a fluorescence or luminescence biology sample [ Maire et al , 2000; Meyer , 1979]. It can also be used to suppress halo or shading in the case of images acquisition.…”

Section: Introductionmentioning

confidence: 99%

The Black Top Hat function applied to a DEM: A tool to estimate recent incision in a mountainous watershed (Estibère Watershed, Central Pyrenees)

Rodriguez

Maire

Courjault‐Radé

et al. 2002

Geophysical Research Letters

Self Cite

View full text Add to dashboard Cite

[1] The Top Hat Transform function is a grey-level image analysis tool that allows extracting peaks and valleys in a nonuniform background. This function can be applied onto a greylevel Digital Elevation Model (DEM). It is herein applied to quantify the volume of recent incised material in a mountainous Pyrenean watershed. Grey-level Closing operation applied to the Present-Day DEM gives a new image called ''paleo'' DEM. The Black Top Hat function consists in the subtraction of the ''paleo'' DEM with the Present-Day DEM. It gives a new DEM representing all valleys whose sizes range between the size of the structuring element and the null value as no threshold is used. The calculation of the incised volume is directly derived from the subtraction between the two DEM's. The geological significance of the quantitative results is discussed.

show abstract

Section: Introductionmentioning

confidence: 99%

The Black Top Hat function applied to a DEM: A tool to estimate recent incision in a mountainous watershed (Estibère Watershed, Central Pyrenees)

Rodriguez

Maire

Courjault‐Radé

et al. 2002

Geophysical Research Letters

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is a major application of this mathematical morphology, which is a set-theoretic method of image analysis providing a quantitative description of geometrical structure (Matheron and Serra 1988;Vogt 1994). The efficiency of Top Hat Transform was firstly introduced by Meyer in 1979 for Cytology applications (Meyer 1979), then it was applied to help with biology sample selection (Maire et al 2000) and images acquisition (Serra and Soille 1994). One of Figure 1.…”

Section: White Top Hat Transformmentioning

confidence: 99%

Asymmetric post-spreading magmatism in the South China Sea: based on the quantification of the volume and its spatiotemporal distribution of the seamounts

Zhao

Ding

Yin

et al. 2019

International Geology Review

View full text Add to dashboard Cite

The volume of seamounts is an essential indicator of the intensity of mantle convection during the evolution of the oceanic lithosphere. Drilling and dredging samples suggest volcanic seamounts are widely distributed in the oceanic basin of the South China Sea, and most of them were formed after cessation of seafloor spreading. By using an SRTM15_PLUS Digital Elevation Model with a 15-arc-second grid, we developed a spatial filtering method based on the Top Hat Transform to extract seamounts. With a combined analysis of basalt dating results from previous studies and gravity anomaly data, an accurate estimation of seamount volume and its spatiotemporal distribution have been obtained. In addition to an asymmetric distribution of the seamounts with a larger volume in the northern flank, clusters of seamounts can be observed at certain locations in the abyssal plain. Due to the consistency between the distribution of the seamount volume and the seafloor spreading features in the South China Sea basin, we propose the ridge jump may induce additional partial melting zones which account for the larger number of seamounts developed in the northern flank while the reorientation of the extension during seafloor spreading dominated the distribution of post-spreading magmatism. Similar to other marginal basins, magmas formed by spontaneous partial melting would migrate through weak lithosphere where the extension direction changed, resulting in post-spreading magmatism in those lithospheric weak zones.

show abstract

“…24 A number of reporter molecules with improved activity have been developed over the past 2 decades. 25 Light emitted by these molecules is detected using advanced photon detectors in CCD cameras mounted within light-tight specimen chambers. 26 Macroscopic in vivo biophonic imaging is a highly sensitive, non-invasive, non-toxic and quantitative optical technique that is based on the detection of light produced by either fluorescent proteins or by enzyme-catalyzed oxidation reactions (bioluminescence).…”

Section: Reporter Systemsmentioning

confidence: 99%

“…22,31,32 Other well established imaging systems include magnetic resonance imaging (MRI) and positron emission tomography. 25,33 In vivo Fluorescence…”

Section: Reporter Systemsmentioning

confidence: 99%

Reporter systems forin vivotracking of lactic acid bacteria in animal model studies

2015

View full text Add to dashboard Cite

Bioluminescence (BLI) and fluorescence imaging (FI) allow for non-invasive detection of viable microorganisms from within living tissue and are thus ideally suited for in vivo probiotic studies. Highly sensitive optical imaging techniques detect signals from the excitation of fluorescent proteins, or luciferase-catalyzed oxidation reactions. The excellent relation between microbial numbers and photon emission allow for quantification of tagged bacteria in vivo with extreme accuracy. More information is gained over a shorter period compared to traditional pre-clinical animal studies. The review summarizes the latest advances in in vivo bioluminescence and fluorescence imaging and points out the advantages and limitations of different techniques. The practical application of BLI and FI in the tracking of lactic acid bacteria in animal models is addressed.

show abstract

Development of an Ultralow-Light-Level Luminescence Image Analysis System for Dynamic Measurements of Transcriptional Activity in Living and Migrating Cells

Cited by 11 publications

References 22 publications

The Black Top Hat function applied to a DEM: A tool to estimate recent incision in a mountainous watershed (Estibère Watershed, Central Pyrenees)

The Black Top Hat function applied to a DEM: A tool to estimate recent incision in a mountainous watershed (Estibère Watershed, Central Pyrenees)

Asymmetric post-spreading magmatism in the South China Sea: based on the quantification of the volume and its spatiotemporal distribution of the seamounts

Reporter systems forin vivotracking of lactic acid bacteria in animal model studies

Contact Info

Product

Resources

About