Development of an ultra‐high‐performance liquid chromatography–tandem mass spectrometry method for the simultaneous determination of crassicauline A, fuziline, karacoline, and songorine in rat plasma and application in their pharmacokinetics

Abstract

In this paper, an ultra‐high‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed for quantifying the levels of crassicauline A, fuziline, karacoline, and songorine in rat plasma. After processing the rat plasma, the proteins in the plasma were separated by extracting the analytes with acetonitrile–methanol (9:1, v/v). The chromatographic column used was the UPLC HSS T3 column, and the mobile phase (methanol–water with 0.1% formic acid) under a gradient elution profile was used to separate the four compounds, with elution times for each analyte being less than 5 min. Electrospray ionization in positive‐ion mode and operating in multiple reaction monitoring mode was used for quantitative analysis. Crassicauline A, fuziline, karacoline, and songorine were administered to 48 rats (n = 6 per group) orally (5 mg/kg) and intravenously (0.5 mg/kg). The standard curves demonstrated excellent linearity in the range of 1–2500 ng/mL, wherein all r values were greater than 0.99. The UPLC–MS/MS method for the determination of crassicauline A, fuziline, karacoline, and songorine in rat plasma was successfully applied in determining their pharmacokinetics parameters, from which their oral bioavailabilities were calculated to be 18.7%, 4.3%, 6.0%, and 8.4%, respectively.

Quantification of Rhodojaponin II and Rhodojaponin III in Rat Plasma by Ultra‐Performance Liquid Chromatography‐Tandem Mass Spectrometry

“…The UPLC-MS/MS method was validated according to the international regulatory guidelines (selectivity, lower limit of quantitation, accuracy, intraday/interday precision, matrix effect, recovery, and stability) [ 8 , 9 ].…”

Pharmacokinetics of IMM‐H012 in rats using ultra‐performance liquid chromatography‐tandem mass spectrometry

“…research results have played an important role in designing new drugs; optimizing drug administration schedules; improving drug dosage forms; and providing efficient, rapid, long-lasting, low-toxicity, and tolerable drugs with effects (Wang et al, 2024;Watanabe et al, 2024). For drug pharmacokinetic analysis, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) is faster and provides a number of benefits over high-performance liquid chromatography-UV and liquid chromatography-tandem mass spectrometry (Chen et al, 2024;Huang et al, 2022;Lin et al, 2022;Yu et al, 2021). Analyzing the in vivo metabolism of intricate chemical systems is possible, thanks to the potent separation and analysis capability.…”