Development of an optical sensor for chlortetracycline detection based on the fluorescence quenching of l‐tryptophan

Abstract

A simple and selective spectrofluorimetric method for the detection of chlortetracycline (CTC) was studied. In pH 7.4 buffer medium l-tryptophan (l-Trp), applied as the fluorescence probe, interacted with CTC resulting in fluorescence quenching of the probe. CTC was detected with maximum excitation and emission wavelengths at λ /λ = 275/350 nm. Notably, quenching of fluorescence intensities was positively proportional to the CTC concentration over the range of 0.65-30 μmol L and the limit of detection was 0.2 μmol L . Effect of temperature shown in Stern-Volmer plots, absorption spectra and fluorescence lifetime determination, indicated that fluorescence quenching of l-Trp by CTC was mainly by static quenching. The proposed study used practical samples analysis satisfactorily.

Carbon Dots Synthesized and its Applications in the Detection of Chlortetracycline and Water Based on the Aggregation‐Induced Emission

“…The detection limit (3 σ / k ) was 3.30 nmol/L, where σ denotes the standard deviation of the blank sample, and k indicates the slope of the linear regression equation. Compared with other sensors for the detection of CTC, as shown in Table , the sensitivity of this method was higher than that of HPLC, immunofluorescence , fluorescence spectroscopy, and the capillary electrophoresis‐electrochemiluminescence method . Meanwhile, the method proposed by us is simpler and economic.…”

“…Accordingly, the detection of CTC is significant to food safety and animal health. The methods to detect CTC primarily include high efficiency liquid chromatography (HPLC), fluorescence spectrophotometry, immunology, capillary electrophoresis and so on. Among them, fluorescence spectrophotometry exhibits more advantages than others, for example, relative simplicity, low cost, rapid detection and high sensitivity.…”