Development of an On-Line Electrochemical Biosensor for Glucose Determination in Rat Brain Using Microdialysis Sampling

Pravda, Miloslav; Kauffmann, Jean‐Michel; Michotte, Yvette

doi:10.1002/1521-4109(200008)12:12<912::aid-elan912>3.0.co;2-z

Cited by 9 publications

(3 citation statements)

References 24 publications

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“…Nevertheless, such kind of amperometric biosensors integrated with in vivo microdialysis are yet useful for the measurement of cerebral microdialysates on the condition that other coexisting compounds do not contribute amperometric interference. To gain a high specificity against AA, several strategies, e.g., coating the biosensor with a layer of an anionic polymer (35), an immobilized ascorbate oxidase layer (37) or electropolymerized membrane (38) have been reported. Moreover, the on-line analytical systems make it possible to readily introduce an electrochemical pre-oxidizing system either in the perfusing line or inside the electrochemical cell to consume AA in the sample and thus suppress its interference to the biosensors (39).…”

Section: Glucose and Lactatementioning

confidence: 99%

Enzyme-based amperometric biosensors for continuous and on-line monitoring of cerebral extracellular microdialysate

Zhang

Mao²

2005

Front Biosci

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Analytical systems integrating in vivo microdialysis sampling with enzyme-based electrochemical biosensor detection have been increasingly accepted to be a new technique for continuous and on-line monitoring of biologically important species. Extensive interests in such integrated on-line analytical systems have suggested that these systems are very useful for physiological and pathological investigations. This review mainly focuses on the principle, development and striking applications of the enzyme-based amperometric biosensors integrated with in vivo microdialysis for continuous and on-line monitoring of cerebral extracellular fluid in recent years.

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Section: Glucose and Lactatementioning

confidence: 99%

Enzyme-based amperometric biosensors for continuous and on-line monitoring of cerebral extracellular microdialysate

Zhang

Mao²

2005

Front Biosci

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“…However, the design and the study of new amperometric biosensors for its determination have received little attention, especially if compared to other compounds of clinical interest such as glucose. [4][5][6][7][8][9] The main challenge when it comes to designing a cholesterol biosensor is finding a measurement system with a long operational lifetime. Other important parameters to take into account are sensitivity, sample throughput and the cost when enzymes are used as bio-recognizing agents for its preparation.…”

Section: Introductionmentioning

confidence: 99%

Development of a Platinized and Ferrocene-Mediated Cholesterol Amperometric Biosensor Based on Electropolymerization of Polypyrrole in a Flow System

2002

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The preparation of a cholesterol amperometric biosensor using a platinized Pt electrode as a support for the electropolymerization of a polypyrrole film, in which cholesterol oxidase and ferrocene monocarboxylic acid (electron-transfer mediator) were co-entrapped, is described. All the biosensor preparation steps (platinization and electropolymerization) and the cholesterol determination take place in the same flow system. The presence of the mediator enhances the sensitivity and selectivity of the platinized biosensor without modifying the dynamic parameters of the response, and the platinized layer improves the operational lifetime of the mediated sensor. The sensitivity obtained was 88.51 nA mM(-1) and the limit of detection was 12.4 microM of cholesterol. The analytical properties of the biosensor for the flow-injection determination of cholesterol were studied and compared with those of other more simple amperometric biosensor configurations.

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“…Moreover, the possibility of modifying the conducting polymer film by introducing suitable functions in the polymer side chains, such as, for example, redox complexes, 21,22 allows one to optimize sensor properties and to design electron-transfer pathways between the immobilized enzyme and the electrode surface. In contrast to conducting polymers, the film thickness achieved by electrochemically induced formation of selflimiting polymers such as polyphenol 23 and poly(o-aminophenol) 24 is small, enabling fast response times. As a consequence of the size exclusion properties of these polymer films, a significant improvement concerning cross talk and direct electrochemical conversion of interfering compounds is observed.…”

mentioning

confidence: 99%

Immobilization Method for the Preparation of Biosensors Based on pH Shift-Induced Deposition of Biomolecule-Containing Polymer Films

2001

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Miniaturization of amperometric biosensors is crucially dependent on the availability of methods for the nonmanual immobilization of biological recognition elements on the transducer surface. From an aqueous polymer suspension, the precipitation of a polymer film with entrapped biological recognition elements is initiated by electrochemically induced oxidation of H20 at the electrode surface. Using the locally generated H+ gradient, acidic side chains of the polymer are titrated, leading to a change in the polymer solubility and hence to the controlled deposition of a polymer film. To investigate the properties and limitations of this immobilization technology, the specific features of a glucose biosensor based on polymer-entrapped glucose oxidase and amperometric detection of enzymatically generated H202 were investigated. Besides the reproducibility of the immobilization procedure, the sensitivity (14.59 mA cm(-2) M(-1) at pH 7), long-term stability (up to 5000 measurements in a sequential-injection analyzer), dependence on enzyme concentration, polymer thickness, and possibilities to fabricate multilayer sensor architectures were exploited. In addition, the miniaturization potential of this nonmanual immobilization technology was evaluated by investigating the modification of microband electrode arrays and cross talk between the neighboring microsensors.

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Development of an On-Line Electrochemical Biosensor for Glucose Determination in Rat Brain Using Microdialysis Sampling

Cited by 9 publications

References 24 publications

Enzyme-based amperometric biosensors for continuous and on-line monitoring of cerebral extracellular microdialysate

Enzyme-based amperometric biosensors for continuous and on-line monitoring of cerebral extracellular microdialysate

Development of a Platinized and Ferrocene-Mediated Cholesterol Amperometric Biosensor Based on Electropolymerization of Polypyrrole in a Flow System

Immobilization Method for the Preparation of Biosensors Based on pH Shift-Induced Deposition of Biomolecule-Containing Polymer Films

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