Development of an oligo(ethylene glycol)-based SPR immunosensor for TNT detection

Mizuta, Yutaka; Onodera, Takeshi; Singh, Praveen; Matsumoto, Kiyoshi; Miura, Norio; Toko, Kiyoshi

doi:10.1016/j.bios.2008.03.042

Cited by 70 publications

(59 citation statements)

References 18 publications

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“…fluidic channel | odorant receptor | robot | Xenopus oocyte V arious approaches to developing artificial odorant detectors have been reported. Presently, chemical sensors are mainly fabricated based on metal-oxide semiconductors, quartz crystal microbalances, surface plasmon resonators, or surface acoustic wave devices (1)(2)(3)(4)(5). These sensors were developed with the aim of achieving good portability while demonstrating high sensitivity and selectivity, in order that they find application as mobile chemical sensors in the monitoring of environmental or physical conditions (6).…”

mentioning

confidence: 99%

Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptors

Misawa

Mitsuno

Kanzaki

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

113

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This paper describes a highly sensitive and selective chemical sensor using living cells (Xenopus laevis oocytes) within a portable fluidic device. We constructed an odorant sensor whose sensitivity is a few parts per billion in solution and can simultaneously distinguish different types of chemicals that have only a slight difference in double bond isomerism or functional group such as ─OH, ─CHO and ─Cð═OÞ─. We developed a semiautomatic method to install cells to the fluidic device and achieved stable and reproducible odorant sensing. In addition, we found that the sensor worked for multiple-target chemicals and can be integrated with a robotic system without any noise reduction systems. Our developed sensor is compact and easy to replace in the system. We believe that the sensor can potentially be incorporated into a portable system for monitoring environmental and physical conditions. fluidic channel | odorant receptor | robot | Xenopus oocyte

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mentioning

confidence: 99%

Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptors

Misawa

Mitsuno

Kanzaki

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

113

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“…(20) The affinity constant of the TNT-Ab-and TNP-gly-immobilized surfaces was 3.0 ×10 7 M −1 , which is the highest among the three kinds of sensor surfaces. (4) As shown in Fig. 3, the slope in the linear region of the TNT response curve for the DNP-aa-modified surface was the best among the three surface types.…”

Section: Detection Of Tntmentioning

confidence: 84%

“…The affinity constants between DNP-aa-TNTAb and DNP-gly-TNT-Ab were 1.0×10 7 M −1 and 2.0×10 7 M −1 , respectively. (4) The DNP-aa-TNT-Ab affinity was slightly weaker than the DNP-gly-TNT-Ab affinity. We expected that the lowest LOD would be realized using the combination with the lowest affinity constant.…”

Section: Detection Of Tntmentioning

confidence: 96%

“…(2,3) In addition, we developed three kinds of sensor surfaces that are highly sensitive for the detection of nitroaromatic compounds. (4)(5)(6) However, the detection time was over 15 min in those studies, because we chose an indirect competitive assay format for highly sensitive detection of explosives. In the assay format, the antibody and antigen solutions are premixed and incubated for about 15 min before measurement.…”

Section: Introductionmentioning

confidence: 99%

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Displacement Immunosensor Based on Surface Plasmon Resonance for Rapid and Highly Sensitive Detection of 2,4,6-Trinitrotoluene

Onodera¹,

Mizuta

Kazuhiko

et al. 2011

Sensors and Materials

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By making use of the antigen-antibody interaction and a surface plasmon resonance (SPR) sensor, we developed a measurement procedure of displacement immunoassay to rapidly detect 2,4,6-trinitrotoluene (TNT). In this procedure, TNT solutions were injected in 30 s at the end of flowing an anti-TNT antibody. Three kinds of sensor surfaces were modified with TNT analogues, namely, TNP-glycine, DNP-glycine, and DNP-acetic acid in a self-assembled monolayer containing ethylene glycol. We investigated which of the TNT analogues provided higher sensitivity using the displacement immunosensor. As a result, the limit of detection (LOD) of TNT was 0.4 ng/mL (ppb) when using the DNP-glycine-modified Au sensor surface with a oneminute flow of TNT solution. We concluded that the final TNT LOD was 0.9 ppb on the basis of experiments using the three different DNP-glycine-modified sensor surfaces. The LOD was 0.7 ppb when using the sensorgram slope 10 s after TNT injection. The displacement immunosensor can detect TNT at sub-ppb levels in 12 s.

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“…For the past few years, we developed highly sensitive SPR immunosensors for TNT, TNP, and DNT detection using an inhibition assay. (2)(3)(4)(5)(6)(7) In these studies, hapten-protein conjugates were often used as constituent materials of SPR sensor chips (2)(3)(4)6) because the threedimensional structure of the surface-bound proteins created an easily accessible binding environment for antibodies, which were fl owed over the sensor chip surfaces, and contributed to the enhancement of SPR response.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Detection of TNT Using a Poly(amidoamine) Dendron-Based SPR Immunosensor

Mizuta

Onodera

Singh

et al. 2010

Sensors and Materials

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In this paper, we describe the fabrication of novel poly(amidoamine) (PAMAM) dendron-based Surface Plasmon Resonance (SPR) sensor chip surfaces supported by a self-assembled monolayer (SAM) of alkanethiol and their use in the highly sensitive detection of 2,4,6-trinitrotoluene (TNT), which is one of main components of explosives. The fabricated surfaces showed excellent binding capacity for TNT antibodies; this is probably due to the fact that the highly branched functional groups and three-dimensional structure of dendron molecules offer densely arranged binding sites and improve binding effi ciency. A limit of detection (LOD) of ca. 15 ppt (pg ml −1 ) was achieved by inhibition assay.

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Development of an oligo(ethylene glycol)-based SPR immunosensor for TNT detection

Cited by 70 publications

References 18 publications

Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptors

Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptors

Displacement Immunosensor Based on Surface Plasmon Resonance for Rapid and Highly Sensitive Detection of 2,4,6-Trinitrotoluene

Highly Sensitive Detection of TNT Using a Poly(amidoamine) Dendron-Based SPR Immunosensor

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