Development of an O-Antigen Serotyping Scheme for
            <i>Cronobacter sakazakii</i>

Sun, Yamin; Wang, Min; Liu, Hongbo; Wang, Jingjing; He, Xin; Zeng, Jing; Guo, Xi; Li, Kun; Cao, Bihua; Wang, Lei

doi:10.1128/aem.02229-10

Cited by 65 publications

(59 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on this approach, the first two O-antigen serotypes were characterized, and denoted as O1 and O2. More recently, Sun et al (2011) added five additional C. sakazakii O-antigen serotypes that correlated with the PCR-RFLP profiles. Jarvis et al (2011) extended the original Mullane molecular characterization scheme to include other Cronobacter spp.…”

Section: Molecular O-antigen Typingmentioning

confidence: 99%

Cronobacter spp. – opportunistic food-borne pathogens. A review of their virulence and environmental-adaptive traits

Jaradat

Mousa

Elbetieha

et al. 2014

Journal of Medical Microbiology

103

View full text Add to dashboard Cite

The genus Cronobacter consists of a diverse group of Gram-negative bacilli and comprises seven species: Cronobacter sakazakii, Cronobacter malonaticus, Cronobacter muytjensii, Cronobacter turicensis, Cronobacter dublinensis, Cronobacter universalis and Cronobacter condimenti. Cronobacter are regarded as opportunistic pathogens, and have been implicated in newborn and infant infections, causing meningitis, necrotizing enterocolitis and bacteraemia or sepsis. Cronobacter virulence is believed to be due to multiple factors. Some strains were found to produce diarrhoea or cause significant fluid accumulation in suckling mice. Two iron acquisition systems (eitCBAD and iucABCD/iutA), Cronobacter plasminogen activator gene (cpa), a 17 kb type VI secretion system (T6SS), and a 27 kb filamentous haemagglutinin gene (fhaBC) and associated putative adhesins locus are harboured on a family of RepFIB-related plasmids (pESA3 and pCTU1), suggesting that these are common virulence plasmids; 98 % of 229 tested Cronobacter strains possessed these plasmids. Even though pESA3 and pCTU1 share a common backbone composed of the repA gene and eitCBAD and iucABCD/iutA gene clusters, the presence of cpa, T6SS and FHA loci depended on species, demonstrating a strong correlation with the presence of virulence traits, plasmid type and species. Other factors were observed, in that Cronobacter form biofilms, and show unusual resistance to heat, dry and acid stress growth conditions. The outer-membrane protein A is probably one of the bestcharacterized virulence markers of Cronobacter. Furthermore, it was reported that Cronobacter employ phosphatidylinositide 3-kinase/Akt signalling, which activates protein kinase C-a and impairs the host cell's mitogen-activated protein kinase pathway, in order to invade cells. Cronobacter can also use immature dendritic cells and macrophages to escape the immune response. This review addresses the various virulence and environmental-adaptive characteristics possessed by members of the genus Cronobacter.

show abstract

Section: Molecular O-antigen Typingmentioning

confidence: 99%

Cronobacter spp. – opportunistic food-borne pathogens. A review of their virulence and environmental-adaptive traits

Jaradat

Mousa

Elbetieha

et al. 2014

Journal of Medical Microbiology

103

View full text Add to dashboard Cite

show abstract

“…In a recent study, serological typing was used to identify five additional C. sakazakii serotypes, bringing the total number of serotypes for this species to seven (42). Other information available about Cronobacter O antigens comes from chemical characterization studies that have identified the repeat polysaccharide sugar composition of five C. sakazakii isolates, one C. malonaticus isolate, and one C. muytjensii isolate (1,2,7,(27)(28)(29)(30).…”

mentioning

confidence: 99%

Molecular Characterization of Cronobacter Lipopolysaccharide O-Antigen Gene Clusters and Development of Serotype-Specific PCR Assays

Jarvis

Grim

Franco

et al. 2011

Appl Environ Microbiol

View full text Add to dashboard Cite

Cronobacter (formerly Enterobacter sakazakii) is a recently defined genus consisting of six species, C. sakazakii, C. malonaticus, C. dublinensis, C. muytjensii, C. turicensis, and Cronobacter genomospecies 1. In this study, MboII restriction fragment length polymorphism (RFLP) patterns of O-antigen gene clusters, located between galF and gnd, were used to identify serotypes in Cronobacter spp. Seven O-antigen RFLP clusters were generated, including three C. sakazakii clusters, previously identified as serotypes O1, O2, and O3. The O-antigen regions of six strains with unique RFLP patterns, including two C. sakazakii strains, two C. malonaticus strains, one C. turicensis strain, and one C. muytjensii strain, revealed three O-antigen gene clusters shared among Cronobacter species. PCR assays were developed, targeting the wzx O-antigen polymerase gene, and used to screen 231 Cronobacter strains to determine the frequency of these newly identified serotypes.

show abstract

“…Research on the organism has considerably progressed in recent years, with improved isolation, identification, and molecular typing methods (22,38,40). These methods have detected considerable diversity within the genus, warranting further detailed investigation.…”

mentioning

confidence: 99%

Diversity of the Cronobacter Genus as Revealed by Multilocus Sequence Typing

et al. 2012

View full text Add to dashboard Cite

Cronobacter (previously known as Enterobacter sakazakii ) is a diverse bacterial genus consisting of seven species: C. sakazakii , C. malonaticus , C. turicensis , C. universalis , C. muytjensii , C. dublinensis , and C. condimenti . In this study, we have used a multilocus sequence typing (MLST) approach employing the alleles of 7 genes ( atpD , fusA , glnS , gltB , gyrB , infB , and ppsA ; total length, 3,036 bp) to investigate the phylogenetic relationship of 325 Cronobacter species isolates. Strains were chosen on the basis of their species, geographic and temporal distribution, source, and clinical outcome. The earliest strain was isolated from milk powder in 1950, and the earliest clinical strain was isolated in 1953. The existence of seven species was supported by MLST. Intraspecific variation ranged from low diversity in C. sakazakii to extensive diversity within some species, such as C. muytjensii and C. dublinensis , including evidence of gene conversion between species. The predominant species from clinical sources was found to be C. sakazakii. C. sakazakii sequence type 4 (ST4) was the predominant sequence type of cerebral spinal fluid isolates from cases of meningitis.

show abstract

Development of an O-Antigen Serotyping Scheme for Cronobacter sakazakii

Cited by 65 publications

References 38 publications

Cronobacter spp. – opportunistic food-borne pathogens. A review of their virulence and environmental-adaptive traits

Cronobacter spp. – opportunistic food-borne pathogens. A review of their virulence and environmental-adaptive traits

Molecular Characterization of Cronobacter Lipopolysaccharide O-Antigen Gene Clusters and Development of Serotype-Specific PCR Assays

Diversity of the Cronobacter Genus as Revealed by Multilocus Sequence Typing

Contact Info

Product

Resources

About