Development of an LC–MS/MS method for the simultaneous quantification of aripiprazole and dehydroaripiprazole in human plasma

Song, Min; Xu, Xinxin; Hang, Taijun; Wen, Aidong; Yang, Lin

doi:10.1016/j.ab.2008.11.027

Cited by 40 publications

(40 citation statements)

References 8 publications

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“…Previous reports have used either liquid-liquid extraction (LLE) [13,15,18,20,21] or solid phase extraction (SPE) [14,19] for quantitative extraction of ARP from human plasma. However, protein precipitation was tried initially (due to high protein binding of the drug) using methanol, ethanol and acetonitrile as precipitating agents, but the response was insufficient with some ion suppression.…”

Section: Methods Developmentmentioning

confidence: 99%

“…Several analytical methods are reported to quantify ARP in different biological matrices, either alone [11][12][13][14][15][16], together with its active metabolite [17][18][19][20][21], or in combination with other antipsychotic and antidepressant drugs [22][23][24][25]. Therapeutic monitoring of ARP in serum samples by highperformance liquid chromatography (HPLC) with column-switching and spectrophotometric detection has been proposed by Kirschbaum and coworkers [11].…”

Section: Introductionmentioning

confidence: 99%

“…The salient features of these methods have been compiled in Table I. Majority of these methods have a sensitivity which is ≥2 ng mL −1 [13-15, 19, 20] with very long chromatographic run time (5-17 min) [13][14][15][18][19][20][21]. Kubo et al [18] have developed a highly sensitive method for ARP and its active metabolite with a linear calibration range of 0.1-100 ng mL −1 .…”

Section: Introductionmentioning

confidence: 99%

“…An improved LC-MS/MS method to determine serum levels of ARP and its metabolite in patients has been proposed by Caloro and co-workers [17]. All other methods [13][14][15][18][19][20][21] describe estimation of ARP in human plasma by gas chromatography-mass spectrometry (GC-MS) [19], capillary electrophoresis [14], HPLC [14,20], or LC-MS/MS [13,15,18,21] techniques. The salient features of these methods have been compiled in Table I.…”

Section: Introductionmentioning

confidence: 99%

“…Kubo et al [18] have developed a highly sensitive method for ARP and its active metabolite with a linear calibration range of 0.1-100 ng mL −1 . An equally sensitive method has been presented by Song et al [21] for the simultaneous quantification of ARP and its active metabolite dehydroaripiprazole, employing 0.5 mL human plasma for sample preparation by liquid-liquid extraction (LLE). The assay was linear over the range of 0.1-600 ng mL −1 for ARP and 0.01-60 ng mL −1 for its metabolite.…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a rapid and sensitive LC-MS/MS method for the determination of aripiprazole in human plasma: Application to a bioequivalence study

Patel¹,

Sharma²,

Patel³

et al. 2014

Acta Chromatographica

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Summary.A rapid, selective, and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay has been proposed for the determination of aripiprazole in human plasma. The analyte and propranolol as internal standard (IS) were extracted from 200 µL of human plasma via liquid-liquid extraction using methyl tert-butyl ether under alkaline conditions. The best chromatographic separation was achieved on an Aquasil C18 (100 × 2.1 mm, 5 µm) column using methanol-deionized water containing 2 mM ammonium trifluoroacetate and 0.02% formic acid (65:35, v/v) as the mobile phase under isocratic conditions. Detection of analyte and IS was done by tandem mass spectrometry, operating in positive ion and multiple reaction monitoring (MRM) acquisition mode. The method was fully validated for its selectivity, interference check, sensitivity, carryover check, linearity, precision and accuracy, reinjection reproducibility, recovery, matrix effect, ion suppression/enhancement, stability, ruggedness, and dilution integrity. The assay was linear over the concentration range of 0.10-100 ng mL −1 for aripiprazole. The intra-batch and inter-batch precision (%CV) was ≤4.8%, while the mean extraction recovery was >96% for aripiprazole across quality control levels. The method was successfully applied to a bioequivalence study of 10 mg aripiprazole orally disintegrating tablet formulation in 27 healthy Indian subjects under fasting and fed condition. The reproducibility in the measurement of study data was demonstrated by reanalysis of 260 incurred samples.

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Section: Methods Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Development and validation of a rapid and sensitive LC-MS/MS method for the determination of aripiprazole in human plasma: Application to a bioequivalence study

Patel¹,

Sharma²,

Patel³

et al. 2014

Acta Chromatographica

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Liquid chromatography–tandem mass spectrometry method for simultaneous quantification of urapidil and aripiprazole in human plasma and its application to human pharmacokinetic study

Reddy

Venugopal

Vemula

et al. 2013

Biomedical Chromatography

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A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of urapidil and aripiprazole in human plasma. A simple liquid-liquid extraction with ethyl acetate was used for the sample preparation. Chromatographic separation was achieved on a Phenomenex C18 (4.6 × 50 mm, 5 µm) column with 0.1% formic acid-acetonitrile (10:90, v/v) as the mobile phase with flow rate of 0.6 mL/min. The quantitation of the target compounds was determined in a positive ion multiple reaction monitoring mode. Calibration plots were linear over the range of 2.0-2503.95 ng/mL for urapidil and 1.0-500.19 ng/mL for aripiprazole. The lower limit of quantitation for urapidil and aripiprazole was 2.0 and 1.0 ng/mL, respectively. Mean recovery was in the range of 69.94-75.62% for both analytes and internal standards. Intra-day and inter-day precisions of the assay at three concentrations were 2.56-5.89% with accuracy of 92.31-97.83% for urapidil, and 3.14-6.84% with accuracy of 91.38-94.42% for aripiprazole. The method was successfully applied to human pharmacokinetic study of urapidil and aripiprazole in healthy human male volunteers.

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Fast simultaneous LC/MS/MS determination of 10 active compounds in human serum for therapeutic drug monitoring in psychiatric medication

Sistik

Urinovska

Brozmanová

et al. 2015

Biomedical Chromatography

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A UPLC/MS/MS method with simple protein precipitation has been validated for the fast simultaneous analysis of agomelatine, asenapine, amisulpride, iloperidone, zotepine, melperone, ziprasidone, vilazodone, aripiprazole and its metabolite dehydro-aripiprazole in human serum. Alprenolol was applied as an internal standard. A BEH C18 (2.1 × 50 mm, 1.7 µm) column provided chromatographic separation of analytes using a binary mobile phase gradient (A, 2 mmol/L ammonium acetate, 0.1% formic acid in 5% acetonitrile, v/v/v; B, 2 mmol/L ammonium acetate, 0.1% formic acid in 95% acetonitrile, v/v/v). Mass spectrometric detection was performed in the positive electrospray ionization mode and ion suppression owing to matrix effects was evaluated. The validation criteria were determined: linearity, precision, accuracy, recovery, limit of detection, limit of quantification, reproducibility and matrix effect. The concentration range was as follows: 0.25-1000 ng/mL for agomelatine; 0.25-100 ng/mL for asenapine and iloperidone; 2.5-1000 ng/mL for amisulpride, aripiprazole, vilazodone and zotepine; 2.3-924.6 ng/mL for dehydroaripiprazole; 2.2-878.4 ng/mL for melperone; and 2.2-883.5 ng/mL for ziprasidone. Limits of quantitation below a therapeutic reference range were achieved for all analytes. Intra-run precision of 0.4-5.5 %, inter-run precision of 0.6-8.2% and overall recovery of 87.9-114.1% were obtained. The validated method was successfully implemented into routine practice for therapeutic drug monitoring in our hospital.

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Development of an LC–MS/MS method for the simultaneous quantification of aripiprazole and dehydroaripiprazole in human plasma

Cited by 40 publications

References 8 publications

Development and validation of a rapid and sensitive LC-MS/MS method for the determination of aripiprazole in human plasma: Application to a bioequivalence study

Development and validation of a rapid and sensitive LC-MS/MS method for the determination of aripiprazole in human plasma: Application to a bioequivalence study

Liquid chromatography–tandem mass spectrometry method for simultaneous quantification of urapidil and aripiprazole in human plasma and its application to human pharmacokinetic study

Fast simultaneous LC/MS/MS determination of 10 active compounds in human serum for therapeutic drug monitoring in psychiatric medication

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