Development of an isotope dilution GC–MS procedure for the routine determination of creatinine in complex serum samples

Fernández‐Fernández, Mario; González-Antuña, Ana; Rodríguez-González, Pablo; Alvarez, Mercedes; Álvarez, Francisco V.; Alonso, J. Ignacio García

doi:10.1016/j.cca.2014.01.011

Cited by 9 publications

(10 citation statements)

References 31 publications

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“…GC-MS has long been used in the clinical laboratory: its use for clinical diagnosis can be traced to the 1970s, with the early focus being on poisoning [ 222 ] and inborn errors of metabolism such as organic aciduria [ 223 , 224 ] and disorders of steroidogenesis [ 225 , 226 ]. It is the basis of definitive and reference methods for many analytes including cholesterol [ 227 ], creatinine [ 228 ], and cortisol [ 229 ]. While it is no longer possible to say that GC-MS is “the most widely used application” [ 74 ] of mass spectrometry in the clinical laboratory, the superior separation and non-selective nature of detection explains the continuing use of GC-MS in urine steroid profiling [ 230 , 231 ], metabolic screening [ 232 , 233 ], and toxicology [ 234 ].…”

Section: Clinical Mass Spectrometrymentioning

confidence: 99%

Clinical Mass Spectrometry in the Bioinformatics Era: A Hitchhiker’s Guide

Chong

Leung

et al. 2018

Computational and Structural Biotechnology Journal

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Mass spectrometry (MS) is a sensitive, specific and versatile analytical technique in the clinical laboratory that has recently undergone rapid development. From initial use in metabolic profiling, it has matured into applications including clinical toxicology assays, target hormone and metabolite quantitation, and more recently, rapid microbial identification and antimicrobial resistance detection by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In this mini-review, we first succinctly outline the basics of clinical mass spectrometry. Examples of hard ionization (electron ionization) and soft ionization (electrospray ionization, MALDI) are presented to demonstrate their clinical applications. Next, a conceptual discourse on mass selection and determination is presented: quadrupole mass filter, time-of-flight mass spectrometer and the Orbitrap; and MS/MS (tandem-in-space, tandem-in-time and data acquisition), illustrated with clinical examples. Current applications in (1) bacterial and fungal identification, antimicrobial susceptibility testing and phylogenetic classification, (2) general unknown urine toxicology screening and expanded new-born metabolic screening and (3) clinical metabolic profiling by gas chromatography are outlined. Finally, major limitations of MS-based techniques, including the technical challenges of matrix effect and isobaric interference; and novel challenges in the post-genomic era, such as protein molecular variants, are critically discussed from the perspective of service laboratories. Computer technology and structural biology have played important roles in the maturation of this field. MS-based techniques have the potential to replace current analytical techniques, and existing expertise and instrument will undergo rapid evolution. Significant automation and adaptation to regulatory requirements are underway. Mass spectrometry is unleashing its potentials in clinical laboratories.

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Section: Clinical Mass Spectrometrymentioning

confidence: 99%

Clinical Mass Spectrometry in the Bioinformatics Era: A Hitchhiker’s Guide

Chong

Leung

et al. 2018

Computational and Structural Biotechnology Journal

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“…In LC-MS/MS, the occurrence of different fragmentation mechanisms may occur simultaneously in the collision cell, and fragment ions containing different number of hydrogen atoms can overlap in the mass spectrum. 35,36 As a consequence, the purity of the selected cluster must be evaluated, following the procedure described in previous work. 18,23 It was observed that the major contribution for selected compounds corresponded to clusters [ In order to apply IPD calculations, it is mandatory to know the exact amount of each labeled compound added to the sample.…”

Section: Measurement Of the Isotopic Composition Of 13 C 12 -Bpa By Lmentioning

confidence: 99%

Method development and validation for the determination of selected endocrine disrupting compounds by liquid chromatography mass spectrometry and isotope pattern deconvolution in water samples. Comparison of two extraction techniques

et al. 2016

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“…Se considera que esta enfermedad afecta a entre 5 -10 % de la población mundial, con un aumento sostenido de la incidencia y prevalencia de la enfermedad renal en etapa terminal, la falla renal y los trasplantes de riñón a nivel mundial (Myers, et al, 2006). De forma rutinaria, este análisis se realiza por métodos colorimétricos como el método de Jaffe, que se ve afectado por interferencias de otros grupos cromóforos provenientes de la bilirrubina, proteínas y cetonas (Fernández-Fernández, et al, 2014). Estas deficiencias se compensan parcialmente tras el desarrollo de métodos enzimáticos (Myers, et al, 2006).…”

Section: Introductionunclassified