Development of an intra-lamellar microdialysis method for laminitis investigations in horses

Antimicrobial drug concentrations and sampling techniques in the equine lung

“…Nevertheless, it is a very reliable tool in tissue pharmacokinetics and has been used in pulmonary pharmacokinetic studies on anaesthetised people (Hutschala et al, 2005) and laboratory animals since 1993 (Eisenberg et al, 1993;Tyvold et al, 2007). Use of MD in the equine lung has not been reported, but studies have been performed in equine muscle tissue (Edner et al, 2005), placenta (Murchie et al, 2006) and hoof lamellar tissue (Nourian et al, 2010).…”

Microdialysis measurements of equine lamellar perfusion and energy metabolism in response to physical and pharmacological manipulations of blood flow

“…A technique for lamellar microdialysis in the horse has previously been described [25,29], and with the use of a modified technique [30] energy metabolites were measured in lamellar dialysate samples and remained relatively stable over a 24 h study period in normal horses. We hypothesised that the microdialysis technique, with urea clearance, would be capable of detecting transient changes in local perfusion and the resultant energy balance within the lamellar tissue in response to interventions expected to cause hyper-or hypoperfusion of the equine digit.…”

Equine laminitis: a tissue microdialysis study of lamellar bioenergetics

“…Recently, the development of an intra-lamellar microdialysis method for continuous monitoring of the ECF in the hoof lamellae, and evaluation of the pharmacokinetics of gentamicin in the lamellar tissue has been reported (Nourian et al, 2010a). Successful determination of gentamicin concentration in lamellar ECF after intravenous (Nourian et al, 2010a) and intraosseous infusion of the distal phalanx (IOIDP) (Nourian et al, 2010b) was achieved.…”

“…Successful determination of gentamicin concentration in lamellar ECF after intravenous (Nourian et al, 2010a) and intraosseous infusion of the distal phalanx (IOIDP) (Nourian et al, 2010b) was achieved.…”

“…A technique for lamellar microdialysis in the horse has previously been described [28], and with the use of a modified technique [29] energy metabolites were measured in lamellar dialysate samples and remained relatively stable over a 24 h study period in normal horses.…”