Development of an Innovative Process for High-Temperature Fruit Juice Extraction Using a Novel Thermophilic Endo-Polygalacturonase From Penicillium oxalicum

Zhong, Cheng; Liu, Xian; Dong, Chen; Lü, Jian; Wei, Yutuo; Du, Liqin; Wang, Qingyan; Chen, Yunlai; Lü, Bo; Bi, Dewu; Zhang, Zhikai; Huang, Ribo

doi:10.3389/fmicb.2020.01200

Cited by 5 publications

(12 citation statements)

References 62 publications

(114 reference statements)

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“…17 However, the optimum pH of 5.0 implies the enzyme was more active in an acidic environment, hence, Chrysophyllum albidum fruit produces acidic polygalacturonase that may be suitable for fruit juice clarification. 21 This finding is in agreement with previous reports on the acidic nature of their extracted polygalacturonase. [21][22][23] Figure 4 shows the optimum temperature of polygalacturonase activity of Chrysophyllum albidum fruit extract was 40°C.…”

Section: Resultssupporting

confidence: 93%

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Partial Purification and Kinetic Properties of Polygalacturonase from Chrysophyllum albidum G. Don Fruit

2021

TJNPR

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Over the years, pectinases have been produced primarily through fermentation with fungal cultures of Aspergillus, Penicillium, Bacillus and Trichoderma species and other sources such as bacteria and yeasts. [12][13][14][15][16] Plant pectinases have high activity and stability in a wide range of temperature and pH. 17 These properties have intensified interest in seeking plant sources of pectinase with good properties for industrial use, which could lead to a more efficient and cheaper yield of fruit juice. Therefore, the present study seeks to characterize and determine the kinetic properties of polygalacturonase from the fruit extract of Chrysophyllum albidum. Materials and Methods Chemicals and ReagentsAll chemicals and reagents of analytical grade were obtained from Sigma-Aldrich Chemie, Klincent and Qualikems Laboratory.

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Section: Resultssupporting

confidence: 93%

“…21 This finding is in agreement with previous reports on the acidic nature of their extracted polygalacturonase. [21][22][23] Figure 4 shows the optimum temperature of polygalacturonase activity of Chrysophyllum albidum fruit extract was 40°C.…”

Section: Resultssupporting

confidence: 93%

Partial Purification and Kinetic Properties of Polygalacturonase from Chrysophyllum albidum G. Don Fruit

2021

TJNPR

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“…The optimum pH and temperature for endo-PGases vary substantially. Most endo-PGases are slightly acidic enzymes with an optimum pH of 2.5–6.0 and an optimum temperature of 30–70 °C [ 17 , 23 ]. Endo-PGases had demonstrated good stability to pH.…”

Section: Resultsmentioning

confidence: 99%

“…Regarding the temperature stability, AnEPG retained 80.7% activity at 40 °C for 120 min and retained only 39.1% activity at 50 °C for 15 min [ 28 ]. PoxaEnPG28B-Pp from Penicillium oxalicum CZ1028 retained 100% activity at 50 °C and 60% activity at 55 °C for 1 h [ 23 ]. However, pePGA was only stable below 45 °C, which needs to be further improved by molecular engineering.…”

Section: Resultsmentioning

confidence: 99%

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A Novel Endo-Polygalacturonase from Penicillium rolfsii with Prebiotics Production Potential: Cloning, Characterization and Application

Hao

et al. 2022

Foods

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In this study, a potential producer of prebiotics, a novel endo-polygalacturonase pePGA from Penicillium rolfsii BM-6, was successfully expressed in Komagataella phaffii, characterized and applied to produce pectic oligosaccharides. The optimum temperature and pH of pePGA were 60 °C and 6.0. The purified recombinant enzyme showed a good pH stability and was stable from pH 3.5 to 8.0. The Km, Vmax and kcat values of pePGA were 0.1569 g/L, 12,273 μmol/min/mg and 7478.4 s−1, respectively. More importantly, pePGA-POS, the pePGA hydrolysis products from commercial pectin, had good prebiotic and antibacterial activities in vitro. The pePGA-POS was able to significantly promote the growth of probiotics; meanwhile, the growth of Escherichia coli JM109, Staphylococcus aureus and Bacillus subtilis 168 was effectively inhibited by pePGA-POS. In addition, pePGA-POS also had the DPPH radical scavenging capacity. These properties of pePGA-POS make pePGA attractive for the production of prebiotics.

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Fungal pectinases: an insight into production, innovations and applications

Dwivedi,

Yadav,

Gupta

et al. 2023

World J Microbiol Biotechnol

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Development of an Innovative Process for High-Temperature Fruit Juice Extraction Using a Novel Thermophilic Endo-Polygalacturonase From Penicillium oxalicum

Cited by 5 publications

References 62 publications

Partial Purification and Kinetic Properties of Polygalacturonase from Chrysophyllum albidum G. Don Fruit

Partial Purification and Kinetic Properties of Polygalacturonase from Chrysophyllum albidum G. Don Fruit

A Novel Endo-Polygalacturonase from Penicillium rolfsii with Prebiotics Production Potential: Cloning, Characterization and Application

Fungal pectinases: an insight into production, innovations and applications

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