Development of an infection‐resistant bifunctionalized Dacron biomaterial

Abstract

A novel infection-resistant biomaterial was created by applying the antibiotic Ciprofloxacin (Cipro) to a recently developed bifunctionalized polyethylene terephthalate ("polyester," Dacron) material using textile-dyeing technology. Dacron was modified via exposure to ethylenediamine (EDA) to create amine and carboxylic acid sites within the polymer backbone. Cipro was applied to the bifunctionalized Dacron construct under varied experimental conditions, with resulting antimicrobial activity determined via zone of inhibition. Dacron segments treated at a liquor ratio of 20:1, with 5% Cipro on weight of fabric (owf), at pH 8 for 4 h at 70 degrees C followed by autoclaving showed antimicrobial activity for 78 days (length of study). Segments treated similarly but without autoclaving lost activity within 1 day. Dyeing time and temperature did not significantly affect antibiotic release/activity, but segments dyed at pHs higher or lower than 8 had less antimicrobial activity. The long-term infection resistance provided by this technique may answer major problems of infection from which implantable Dacron biomedical devices suffer.

Development of an infection‐resistant, bioactive wound dressing surface

“…Additional research by our group has shown Cipro release with subsequent antimicrobial activity to persist for Ͼ40 days. 37 This sustained release is comparable to that observed with the pad-heating processes on unmodified Dacron. 42 The reduced heat involved in this pad/autoclave technique also preserves the functional groups on the material surface, thereby permitting subsequent protein binding using crosslinkers.…”

“…Because the amine and carboxylic acid groups generated by EDA exposure render the Dacron surface more hydrophilic, a modified dyeing technique (pad/autoclaving) was examined. 37 C-EDA segments (3 ϫ 5 cm) were prepared as previously described. Segments were then placed into a Cipro "dyebath" that was set up as follows: liquor ratio (ratio of water weight to material weight) ϭ 20:1, percent antibiotic on the weight of fabric (% owf) ϭ 5%, pH ϭ 8.0, dyeing time ϭ 2 h, and dyeing temperature ϭ 70°C.…”

Analysis of functionalized polyethylene terephthalate with immobilized NTPDase and cysteine

“…This results in reducing the durability of PET in implants and other long-term biomedical applications. Even with these limitations, many studies have demonstrated immobilization of active biomolecules [5,6,16,19,21,22,[24][25][26]. These studies have also showed improved hemocompatibility [6,19,21], infection resistance [5,[24][25][26] and cell adhesion [22].…”

Biocidal efficacy, biofilm-controlling function, and controlled release effect of chloromelamine-based bioresponsive fibrous materials

“…Following the pioneering work of Gagliardi [20], which described the principles and strategies for imparting antimicrobial activities into fibrous materials, various biocidal agents, including antibiotics [21][22][23], metal ions [24], quaternary ammonium salts [25][26][27], phosphonium compounds [27,28], N-halamines [29,30], etc., have been incorporated into fibrous materials. The antimicrobial activities and mechanisms of these agents differ considerably.…”