2009
DOI: 10.1016/j.vetpar.2009.05.029
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Development of an indirect ELISA test using a purified tachyzoite surface antigen SAG1 for sero-diagnosis of canine Toxoplasma gondii infection

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Cited by 35 publications
(37 citation statements)
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“…This antigen is stage specific antigen that only exists in tachyzoite stage of T. gondii (Kasper et al 1984). Hosseininejad et al (2009) showed that ELISA test using SAG1 antigen has a relative sensitivity and specificity of 94.52 and 93.60 %, respectively for diagnosis of T. gondii infection in dogs and does not show cross-reactivity with closely related protozoan parasite, Neospora caninum (Hosseininejad et al 2009). Therefore, in current study ELISA test using SAG1 antigen was selected for diagnosis of T. gondii in dogs.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…This antigen is stage specific antigen that only exists in tachyzoite stage of T. gondii (Kasper et al 1984). Hosseininejad et al (2009) showed that ELISA test using SAG1 antigen has a relative sensitivity and specificity of 94.52 and 93.60 %, respectively for diagnosis of T. gondii infection in dogs and does not show cross-reactivity with closely related protozoan parasite, Neospora caninum (Hosseininejad et al 2009). Therefore, in current study ELISA test using SAG1 antigen was selected for diagnosis of T. gondii in dogs.…”
Section: Discussionmentioning
confidence: 99%
“…To diagnose canine toxoplasmosis, ELISA test using SAG1 antigen shows a reliable sensitivity and specificity. This method is based on IgG antibody detection (Hosseininejad et al 2009). …”
Section: Introductionmentioning
confidence: 99%
“…The methodology can be automated and this makes it attractive for use in large-scale epidemiological studies. For the most recent tests introduced on the market, manufacturers declare sensitivity and specificity greater than IFAT and other serological tests (Hosseininejad et al, 2009). ELISA tests to demonstrate IgG to diagnose active infections are available in the market although there is not full agreement among researchers on their sensitivity and specificity (Shaapan et al, 2008;Hosseininejad et al, 2009).…”
Section: Modified Agglutination Test Immunofluorescence Antibody Tesmentioning
confidence: 99%
“…For the most recent tests introduced on the market, manufacturers declare sensitivity and specificity greater than IFAT and other serological tests (Hosseininejad et al, 2009). ELISA tests to demonstrate IgG to diagnose active infections are available in the market although there is not full agreement among researchers on their sensitivity and specificity (Shaapan et al, 2008;Hosseininejad et al, 2009). Recently, an indirect ELISA test with purified tachyzoite surface antigen SAG1 has been proposed (Hosseininejad et al, 2009).…”
Section: Modified Agglutination Test Immunofluorescence Antibody Tesmentioning
confidence: 99%
See 1 more Smart Citation