Development of an indirect ELISA test using a purified tachyzoite surface antigen SAG1 for sero-diagnosis of canine Toxoplasma gondii infection

Hosseininejad, M.; Azizi, Hamidreza; Hosseini, Fatemeh; Schares, Gereon

doi:10.1016/j.vetpar.2009.05.029

Cited by 35 publications

(37 citation statements)

References 22 publications

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“…This antigen is stage specific antigen that only exists in tachyzoite stage of T. gondii (Kasper et al 1984). Hosseininejad et al (2009) showed that ELISA test using SAG1 antigen has a relative sensitivity and specificity of 94.52 and 93.60 %, respectively for diagnosis of T. gondii infection in dogs and does not show cross-reactivity with closely related protozoan parasite, Neospora caninum (Hosseininejad et al 2009). Therefore, in current study ELISA test using SAG1 antigen was selected for diagnosis of T. gondii in dogs.…”

Section: Discussionmentioning

confidence: 99%

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Seroprevalence of Toxoplasma gondii infection in domestic dogs in an area from northwest of Iran: a cross-sectional study using immunodominant surface antigen 1 (SAG1)

et al. 2015

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Toxoplasma gondii is an obligate intracellular protozoan parasite that infects humans and animals. T. gondii surface antigen 1 (SAG1) is an appropriate antigen with high specificity and sensitivity for the detection of T. gondii infection in humans and animal hosts. The aim of this study was to determine the seroprevalence of T. gondii infection using SAG1 antigen (P30) in ownership dogs in Meshkin-Shahr district in the northwestern Iran. The sera samples were collected from 171 domestic dogs and tested using indirect ELISA (SAG1 antigen). The data were analyzed using SPSS software version 13. From a total of 171 dogs, 82 (48 %) of them were sero-positive. No statistical significant difference was seen between T. gondii infection and gender (P = 0.995). The highest seroprevalence of rate was observed in [5 years animals; but no statistical significant difference was seen between T. gondii infection and age (P = 0.589). Our findings indicate that Toxoplasma seropositivity rate is high in ownership dogs in northwest of Iran. This is probably due to high exposure to contaminated food, soil, or water sources with sporulated Toxoplasma oocysts.

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Section: Discussionmentioning

confidence: 99%

“…To diagnose canine toxoplasmosis, ELISA test using SAG1 antigen shows a reliable sensitivity and specificity. This method is based on IgG antibody detection (Hosseininejad et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Toxoplasma gondii infection in domestic dogs in an area from northwest of Iran: a cross-sectional study using immunodominant surface antigen 1 (SAG1)

et al. 2015

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“…The methodology can be automated and this makes it attractive for use in large-scale epidemiological studies. For the most recent tests introduced on the market, manufacturers declare sensitivity and specificity greater than IFAT and other serological tests (Hosseininejad et al, 2009). ELISA tests to demonstrate IgG to diagnose active infections are available in the market although there is not full agreement among researchers on their sensitivity and specificity (Shaapan et al, 2008;Hosseininejad et al, 2009).…”

Section: Modified Agglutination Test Immunofluorescence Antibody Tesmentioning

confidence: 99%

“…For the most recent tests introduced on the market, manufacturers declare sensitivity and specificity greater than IFAT and other serological tests (Hosseininejad et al, 2009). ELISA tests to demonstrate IgG to diagnose active infections are available in the market although there is not full agreement among researchers on their sensitivity and specificity (Shaapan et al, 2008;Hosseininejad et al, 2009). Recently, an indirect ELISA test with purified tachyzoite surface antigen SAG1 has been proposed (Hosseininejad et al, 2009).…”

Section: Modified Agglutination Test Immunofluorescence Antibody Tesmentioning

confidence: 99%

“…ELISA tests to demonstrate IgG to diagnose active infections are available in the market although there is not full agreement among researchers on their sensitivity and specificity (Shaapan et al, 2008;Hosseininejad et al, 2009). Recently, an indirect ELISA test with purified tachyzoite surface antigen SAG1 has been proposed (Hosseininejad et al, 2009). SAG1 antigen is stage-specific and only detectable in the tachyzoite stage (Pietkiewicz et al, 2007).…”

Section: Modified Agglutination Test Immunofluorescence Antibody Tesmentioning

confidence: 99%

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Toxoplasma in Animals, Food, and Humans: An Old Parasite of New Concern

Cenci‐Goga

Rossitto

Sechi

et al. 2011

Foodborne Pathogens and Disease

137

106

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All hosts, including humans, can be infected by any one of the three forms of the parasite Toxoplasma gondii that correspond to three morphological stages: tachyzoite, bradyzoite, and sporozoite form. Felids are definitive hosts for T. gondii, which is an intracellular pathogen that infects a wide range of warm-blooded intermediate hosts. Toxoplasmosis is a disease where the interest of the diverse medical and veterinary specialties converge. Awareness needs to be increased that toxoplasmosis can induce clinical disease not only in immunocompromised patients or through congenital infections, but also in healthy patients. This is a review article that aims at illustrating why toxoplasmosis should be regarded a veterinary public health issue and how veterinary practitioners can contribute in controlling the infection.

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Prevalence and molecular characterization of Toxoplasma gondii in different types of poultry in Greece, associated risk factors and co-existence with Eimeria spp.

et al. 2022

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Toxoplasma gondii is a protozoan parasite of public health importance, infecting all warm-blooded animals, including chickens. Undercooked chicken meat or relevant products such as sausages could lead to human infections. In free-range, organic and slow-growth farming systems where the susceptibility period for chickens is extended, more knowledge about potential risk factors is essential. This study is the first seroepidemiological survey in different regions and types of chicken farms in Greece, using a major tachyzoite surface antigen-based ELISA (TgSAG1), combined with magnetic-capture PCR (mc-PCR) and bioassay for the isolation of strains from the chickens’ tissues. Potential risk factors for T. gondii infection in these hosts were also investigated. Additionally, the co-existence of T. gondii and Eimeria spp. infections was assessed to elucidate epidemiological links between these two protozoan infections. Overall T. gondii seroprevalence was 9.5%. Of the backyard chickens sampled, 41.2% were seropositive and 70% of the organic and free-range layer farms had at least one T. gondii seropositive hen. No serologically positive broilers were found, although mc-PCR revealed a positive sample, highlighting the importance of accurate early-infection direct detection of T. gondii infections to ensure public health. T. gondii isolates obtained by mouse bioassay were genotyped. All belonged to type II (ToxoDB#3) as confirmed also by microsatellite typing. Production system, type of nutrition, and feeding system automation were identified as the most significant risk factors, while no association was found between the presence of cats and T. gondii seropositivity as calculated on both a farm level and per individual bird sampled.

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Development of an indirect ELISA test using a purified tachyzoite surface antigen SAG1 for sero-diagnosis of canine Toxoplasma gondii infection

Cited by 35 publications

References 22 publications

Seroprevalence of Toxoplasma gondii infection in domestic dogs in an area from northwest of Iran: a cross-sectional study using immunodominant surface antigen 1 (SAG1)

Seroprevalence of Toxoplasma gondii infection in domestic dogs in an area from northwest of Iran: a cross-sectional study using immunodominant surface antigen 1 (SAG1)

Toxoplasma in Animals, Food, and Humans: An Old Parasite of New Concern

Prevalence and molecular characterization of Toxoplasma gondii in different types of poultry in Greece, associated risk factors and co-existence with Eimeria spp.

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