Development of an indirect ELISA test using an affinity purified surface antigen (P38) for sero-diagnosis of canine Neospora caninum infection

Hosseininejad, M.; Hosseini, Fatemeh; Mosharraf, M.; Shahbaz, Shima; Mahzounieh, Mohammadreza; Schares, Gereon

doi:10.1016/j.vetpar.2010.04.003

Cited by 12 publications

(2 citation statements)

References 32 publications

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“…Recent surveys showed that the number of T. gondii seropositive dogs was as low as 21% in China (Zhang et al 2010) and as high as 69.8% in Brazil (Valadas et al 2010). For N. caninum, the level ranged from 1.9% in the West Indies (Dubey et al 2008), to an intermediate percentage of 8.4% in Northeast Brazil (Azevedo et al 2005), and to the highest value of 32% in Iran (Hosseininejad et al 2010). However, the reported comparison of prevalence data depends on numerous factors, such as the number, type and origin of dogs tested, and the sensitivity and specificity of the serological tests applied (Dubey et al 1985, Silva et al 1997.…”

Section: Dog Toxoplasma Gondii Neospora Caninum Seroprevalence Koreamentioning

confidence: 99%

Seroprevalence of Toxoplasma gondii and Neospora caninum in dogs from Korea

Nguyen

Choe

Byun

et al. 2012

Acta Parasitologica

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Toxoplasma gondii and Neospora caninum are closely related protozoan parasites, they share many common hosts, and can cause neurological diseases in dogs. Dogs can have close contacts with humans and livestock and therefore they can act as reservoirs of these parasites. The aim of this study was to survey the seroprevalence of antibodies against T. gondii and N. caninum and their co-infection rate in dogs in Korea. In total, sera from 553 domestic dogs were collected from different breeds, sexes, and ages of dogs from nine provinces across the country of Korea during 2006 and 2007. The presence of antibodies against T. gondii and N. caninum was analyzed using the latex agglutination test (LAT) with a cut-off value of 1:32, and the indirect fluorescent antibody test (IFAT) using a serum titer of 1:100. In the total dog population, 71 (12.8%) dogs were positive for anti-T. gondii antibodies and only 20 (3.6%) were positive for anti-N. caninum antibodies. Relatively higher seropositive frequencies of antibodies against T. gondii (20.1%) and N. caninum (4.9%) were detected in the dog population from the Gyeonggi. A higher proportion of animals seropositive for anti-T. gondii antibodies was found in stray dog populations as compared to household dog populations: 18.5% (59/319) vs 5.1% (12/234), respectively. The Chi-square tests revealed significant differences in the seropositive frequencies of antibodies against T. gondii between stray and household dogs in the total population (p<0.0001), and in dogs from the Gyeonggi (p<0.01). No significant differences were observed for the presence of antibodies against T. gondii or N. caninum when compared across the sex or age (p>0.05). The first serological survey on antibodies against both T. gondii and N. caninum parasites across the entire country showed that co-infection was not common in these canine populations with a seropositive level of 0.72%. The significantly higher positive frequency of T. gondii antibodies in stray dogs in both, Gyeonggi and in the total dog populations suggests that further investigation on the seroprevalence of parasites should focus on stray dogs.

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Section: Dog Toxoplasma Gondii Neospora Caninum Seroprevalence Koreamentioning

confidence: 99%

Seroprevalence of Toxoplasma gondii and Neospora caninum in dogs from Korea

Nguyen

Choe

Byun

et al. 2012

Acta Parasitologica

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“…The surface proteins of N. caninum NcSRS2 and NcSAG1 are immunodominant antigens that play a key role in the host cell recognition, adhesion, and invasion ( 14 , 15 ). Jenkins et al ( 16 ) investigated the immune effect of plasmid DNA encoding NcGRA6 and NcGRA7, and confirmed that NcGRA7 can be used as an immunogen in the prevention of neosporosis ( 16 ).…”

Section: Introductionmentioning

confidence: 99%

Development and application of an indirect ELISA to detect antibodies to Neospora caninum in cattle based on a chimeric protein rSRS2-SAG1-GRA7

Yang

Ayanniyi

et al. 2022

Front. Vet. Sci.

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Neospora caninum is an important apicomplexan parasite causing neosporosis in cattle. The disease is recognized as one of the most important cause of reproductive problems and abortion in cattle worldwide. In this context, we developed an indirect enzyme-linked immunosorbent assays (ELISA) with chimeric protein rSRS2-SAG1-GRA7 to diagnose antibodies to Neospora-infection. This indirect ELISA was compared to indirect fluorescent antibody test (IFAT) and western blotting (WB), and the sensitivity and specificity results of ELISA were calculated to be 86.7 and 96.1%, respectively. The overall coincidence rate was 92.6% using IFAT and WB. Additionally, 329 aborting dairy cattle serum samples were tested using this ELISA to evaluate the prevalence of N. caninum in Ningxia, China. The positive rate of N. caninum in these farms was from 19.05 to 57.89%, and the mean rate was 41.64% (±11.01%), indicating that infection with N. caninum may be one of the important causes of cattle abortion in this region. This established rSRS2-SAG1-GRA7 indirect ELISA is capable for detecting the antibodies against N. caninum, and it could be a useful screening tool for monitoring the epidemiology of neosporosis in cattle.

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Neospora

Moore¹,

Venturini²

2017

Parasitic Protozoa of Farm Animals and Pets

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Development of an indirect ELISA test using an affinity purified surface antigen (P38) for sero-diagnosis of canine Neospora caninum infection

Cited by 12 publications

References 32 publications

Seroprevalence of Toxoplasma gondii and Neospora caninum in dogs from Korea

Seroprevalence of Toxoplasma gondii and Neospora caninum in dogs from Korea

Development and application of an indirect ELISA to detect antibodies to Neospora caninum in cattle based on a chimeric protein rSRS2-SAG1-GRA7

Neospora

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