Development of an in vitro Environmental Monitoring System by Using Immune Cells.

Shim, Kyoo Jung; Jung, Kyu Hyuck; Chung, Myung Kiu; Choung, Se‐Young

doi:10.1248/jhs.48.130

Cited by 6 publications

(4 citation statements)

References 6 publications

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“…Macrophage was separated from the head kidney following the modification of the method by Shim, Jung, Chung, and Choung (), which was as follows: the head kidney was removed from the fish aseptically. After washing twice with Hank's balanced salt solution, the head kidney was cut into small fragments by ophthalmic scissors.…”

Section: Methodsmentioning

confidence: 99%

A comprehensive evaluation of replacing fishmeal with housefly (Musca domestica ) maggot meal in the diet of Nile tilapia (Oreochromis niloticus ): growth performance, flesh quality, innate immunity and water environment

Wang

Jin

et al. 2017

Aquacult Nutr

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A 10‐week feeding trial of using housefly (Musca domestica) maggot meal (MM) in practical feeds for Nile tilapia (Oreochromis niloticus) was conducted to assess the growth performance, ingredient utilization, flesh quality, innate immunity and its influence on water environment. Fish were fed five isonitrogenous and isoenergetic diets, where fishmeal (FM) was substituted by MM at the level of 0, 90, 180, 270 and 360 g kg‐1 diet (remaining FM content: 360, 270, 180, 90 and 0 g kg‐1). There was no significant difference in feed intake and apparent digestibility coefficient between the treatments. Replacing up to 270 g kg‐1 FM did not have an impact on the growth performance and ingredient utilization, whereas the complete replacement of FM caused significantly lower survival rate, weight gain, specific growth rate and higher feed conversion rate. Dietary MM was also proved positively influential in flesh quality, whereas replacing 180 g kg‐1 or more FM suppressed the innate immunity of tilapia. When compared by the effects on the water environment, the increasing substitute levels were accompanied with the declining concentrations of nitrite nitrogen and total phosphorus in the water. Our study verified the feasibility of using MM as a partial substitute of FM in aquatic feed. When replacing 180 g kg‐1 FM (corresponding to half of the FM content in control diet) in the diet of Nile tilapia, it can serve as a renewable and environmentally superior alternative without compromising the performance criteria.

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Section: Methodsmentioning

confidence: 99%

A comprehensive evaluation of replacing fishmeal with housefly (Musca domestica ) maggot meal in the diet of Nile tilapia (Oreochromis niloticus ): growth performance, flesh quality, innate immunity and water environment

Wang

Jin

et al. 2017

Aquacult Nutr

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“…The usefulness of immune assays developed in laboratory fish models as monitors of pollution effects on wild fish has recently been validated by Zelikoff et al (2002). A further recent development of note is the observation by Shim et al (2002) that the phagocytic abilities of macrophages of Sprague-Dawley rats and fish (Cyprinus carpio and Carassius auratus) respond similarly to exposure to polluted water. This led the authors to propose that the greater availability of rat macrophages would make them more useful than fish macrophages as a monitor of water pollution.…”

Section: Introductionmentioning

confidence: 99%

Inflammatory interactions in fish exposed to pollutants and parasites: a role for apoptosis and C reactive protein

et al. 2003

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Although previous studies have highlighted the inflammatory responses of fish infected with parasites and exposed to pollutants, very little is known about how these two stressors interact within the fish. In this review, which also contains original data, the effect of these two parameters on the fish inflammatory response is assessed and, in particular, the role of apoptosis and the acute phase protein, C reactive protein, is evaluated. In Cyprinus carpio exposed to 0.5 mg NH4+ l(-1) or 0.1 mg Cd2+ l(-1) and experimentally infected with the blood fluke, Sanguinicola inermis, the pollutant type and the order in which the fish experiences the parasite and toxicant, significantly affects the ultrastructural appearance and cellular content of the pronephros and thymus. This is reflected in the intensity of infection where the pollutant appears to have less effect on an established infection. Both stressors, pollutant and infection, may mediate their effects via the endocrine system. Studies have revealed that cortisol at 100 ng ml(-1) is able to induce apoptosis in pronephric cells of carp and that an increase in apoptosis is associated with an increase in phagocytosis in this immune organ. In addition, C reactive protein, which is used as a biomarker of the inflammatory response in humans and other mammals, is evaluated as a possible indicator of physiological states in fish exposed to pathogens and pollutants.

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“…Moreover, the evidence is lacking to draw superior sensitive species in immunotoxic detection. Shim et al 36 used phagocytosis of rat macrophages to indicate the pollution levels of several rivers in southern Korea. They found that 40-fold concentrated water obviously inhibited phagocytosis (45−49%), which was close to our findings.…”

Section: Effects Of the Water Sample Extracts On The Phagocytic Activ...mentioning

confidence: 99%

In Vitro Multibiomarker Approaches for Assessing the Immunotoxicity of Certain Sections in Yangtze River

et al. 2022

ACS EST Water

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Due to multiple endpoints and complex mechanisms, immunotoxicity tests were scarcely applied to environmental matrices. In this study, using a high-content screening system, a series of high-throughput multibiomarker approaches based on the immune functions of THP-1 macrophages (cell adhesion, morphology, phagocytosis, and production of multiple cytokines/chemokines) were adopted to evaluate the immunotoxicity of surface water extracts from the Yangtze River (near the Nanjing Chemical Industrial Park). The results suggested that the cell morphology and adhesion of macrophages were sensitive and appropriate for evaluating the surface water quality since both were closely associated with inflammation initiation and altered significantly when treated with only 10× water extracts. Twenty-four hours of exposure with water organic extracts (10× and 50×) suppressed cell phagocytosis, which was similar to the results of surface water extracts from southern Korea. Additionally, 30 cytokines/chemokines (including inflammatory cytokines; mediators of macrophages, T cells, and other immune cells; regulating neurons, angiogenesis, and anti-infection processes) were significantly altered by 50× water extracts. The top 10 cytokines are recommended as potential candidates for indicating surface water immunotoxicity. This research is the first study to evaluate many immunotoxicity endpoints with surface water extracts and offers fresh insights into the in vitro immunotoxic evaluation methods.

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Development of an in vitro Environmental Monitoring System by Using Immune Cells.

Cited by 6 publications

References 6 publications

A comprehensive evaluation of replacing fishmeal with housefly (Musca domestica ) maggot meal in the diet of Nile tilapia (Oreochromis niloticus ): growth performance, flesh quality, innate immunity and water environment

A comprehensive evaluation of replacing fishmeal with housefly (Musca domestica ) maggot meal in the diet of Nile tilapia (Oreochromis niloticus ): growth performance, flesh quality, innate immunity and water environment

Inflammatory interactions in fish exposed to pollutants and parasites: a role for apoptosis and C reactive protein

In Vitro Multibiomarker Approaches for Assessing the Immunotoxicity of Certain Sections in Yangtze River

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