Development of an IMS-PCR assay for the detection of Mycobacterium avium ssp. paratuberculosis in water

Whan, Lynne; Ball, H.J.; Grant, Irene R.; Rowe, M.T.

doi:10.1111/j.1472-765x.2005.01662.x

Cited by 19 publications

(8 citation statements)

References 18 publications

(26 reference statements)

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“…We have previously experienced difficulty in removing M. avium subsp. paratuberculosis from filters (32), and this may have been one factor contributing to the nonrecovery of M. avium subsp. paratuberculosis by Hunter et al (18).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Mycobacterium avium subsp. paratuberculosis in Untreated Water in Northern Ireland

Whan

Ball²,

Grant

et al. 2005

Appl Environ Microbiol

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Mycobacterium avium subsp. paratuberculosis is the known cause of Johne's disease of both domestic and wild ruminants and has been implicated as a possible cause of Crohn's disease in humans. The organism is shed in the feces of infected animals and can survive for protracted periods in the environment and hence could be present in catchment areas receiving agricultural runoff. A limited survey was undertaken in Northern Ireland to test for M. avium subsp. paratuberculosis in untreated water entering nine water treatment works (WTWs) over a 1-year period. Three detection methods were employed, viz., immunomagnetic separation-PCR and culture on Herrold's egg yolk medium (HEYM) and BACTEC 12B medium, the latter both supplemented with mycobactins. Of the 192 untreated water samples tested, 15 (8%) tested M. avium subsp. paratuberculosis positive by one or more of the three detection methods. M. avium subsp. paratuberculosis was successfully isolated from eight untreated water samples, three by BACTEC culture and five by culture on HEYM. Although the highest incidence of M. avium subsp. paratuberculosis was found in spring, overall, there was no statistically significant difference between the seasons. No significant correlation was found between numbers of coliforms or fecal coliforms and the presence of M. avium subsp. paratuberculosis. In general, a higher incidence of M. avium subsp. paratuberculosis was found in untreated water entering those WTWs that had a high mean water pH value over the sampling period. This work indicates the need to determine the efficacy of water treatment processes to either kill or remove M. avium subsp. paratuberculosis from untreated water and the possible risks posed by contact with recreational water sources.

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Section: Discussionmentioning

confidence: 99%

Occurrence of Mycobacterium avium subsp. paratuberculosis in Untreated Water in Northern Ireland

Whan

Ball²,

Grant

et al. 2005

Appl Environ Microbiol

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“…IS900 is specific for M. avium subsp. paratuberculosis and is the most widely used sequence for its detection (Pickup et al 2005(Pickup et al , 2006Whan et al 2005;Torvinen et al 2010). For detection of M. avium subsp.…”

Section: Identification Of Mycobacteriamentioning

confidence: 99%

Mycobacteria in water, soil, plants and air: a review

Hruška¹,

Kaevska²

2012

Vet. Med.

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Amazingly, despite the 24 143 papers on mycobacteria, indexed in the Web of Science database during the last six years, published by 67 008 authors from 13 128 organizations located in 166 countries or territories, internationally accepted legal directives on how to control the public health risk associated with environmental mycobacteria have yet to be developed. Mycobacteria are human and animal pathogens, causing not only tuberculosis and leprosy, but mycobacterioses of skin, soft tissues and lung. Due to their cell wall composition and their adaptability mycobacteria can survive in different habitats for years. Their immunomodulatory ability has been recognised for more than 50 years and hundreds of papers published during the last two decades have demonstrated that small chemical products derived from mycobacterial cells participate in inflammatory pathways involved the pathogenesis of important human diseases like Crohn's disease, asthma, type 1 diabetes mellitus, psoriasis, arthrosis, Blau syndrom, sarcoidosis, autism etc. Mycobacteria can influence inflammatory pathways not only as live organisms, but also by means of components derived from dead cells. Pasteurisation or cooking does not affect this ability. Hence, how many mycobacterial cells are ingested, what factors play a role concurrently, and how long the harmful effect persists become important questions. This paper presents only a short review based on selected papers about mycobacteria in water, soil, plants and air with the aim of attracting attention to this significant global problem and of making the first steps towards protection of people. Selected bibliographic references of published data from 2007 to 2012 are presented in easy-to-navigate tables.Keywords: Mycobacterium; water; soil; plant; vegetables; air; biofilm; sediment; determination; zoonoses; food safety List of abbreviations CFU = colony forming units; DGGE = denaturation gradient gel electrophoresis; IMS = immuno-magnetic separation; IS = insertion sequence; ITS = intergenic transcribed spacer; MAC = Mycobacterium avium complex; MAP/Map = Mycobacterium avium subsp. paratuberculosis; MTC = Mycobacterium tuberculosis complex; MWF = metal working fluids; nPCR = nested PCR; NTM = non-tuberculous mycobacteria; PCR = polymerase chain reaction; PPM = potentially pathogenic mycobacteria; qPCR = quantitative real time PCR; RFLP = restriction fragment length polymorphism

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“…paratuberculosis among high levels of other environmental bacteria is beneficial (7). Such samples include water (31) and bulk milk tank (12,21) samples as well as other environmental samples (8,25). …”

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confidence: 99%

Production and Characterization of Monoclonal Antibodies against a Major Membrane Protein ofMycobacterium aviumsubsp.paratuberculosis

Bannantine

Radosevich

Stabel

et al. 2007

Clin Vaccine Immunol

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Development of an IMS-PCR assay for the detection of Mycobacterium avium ssp. paratuberculosis in water

Cited by 19 publications

References 18 publications

Occurrence of Mycobacterium avium subsp. paratuberculosis in Untreated Water in Northern Ireland

Occurrence of Mycobacterium avium subsp. paratuberculosis in Untreated Water in Northern Ireland

Mycobacteria in water, soil, plants and air: a review

Production and Characterization of Monoclonal Antibodies against a Major Membrane Protein ofMycobacterium aviumsubsp.paratuberculosis

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