Development of an immunomagnetic separation method for efficient enrichment of Escherichia coli O157:H7

Rapid and sensitive detection of Salmonella enteritidis by a pre-concentrated immunochromatographic assay in a large-volume sample system

“…For comparison, the CG-ICA and MNP-ICA were developed and optimized according to previous work. 19,27 The procedure of CG-ICA and MNP-ICA were conducted as follows. A total of 8 mL of ICG or 8 mg of IMNPs was added in 100 mL of a series of S. enteritidis dilutions (1.95 Â 10 3 to 1.95 Â 10 8 CFU mL À1 ), and 100 mL of the mixture was transferred into the test strip.…”

“…17,18 Immunomagnetic separation has been a useful technology to concentrate and separate target pathogens from complex food samples. [19][20][21][22] In recent years, immunomagnetic separation was also coupled with ICA to improve the detection sensitivity and specicity. For example, Cui et al 23 established a method combined immunomagnetic separation with CG-ICA to isolate and detect Escherichia coli O157:H7.…”

An evaluation of immunomagnetic separation-real-time PCR (IMS-RTiPCR) combined assay for rapid and specific detection of Escherichia coli O157:H7 in raw milk and ground beef

“…Escherichia coli O157:H7 (EHEC) which is an enterohemorrhagic serotype of the Shiga toxin-producing E. coli (STEC), is considered a serious public health problem since it is recognized as a major pathogen of foodborne diseases in humans with a capable of causing diseases like diarrhea, hemorrhagic colitis (HC), the potentially fatal hemolytic uremic syndrome (HUS), thrombotic thrombocytopenic purpura (TTP), and kidney failure (Qin et al, 2018;Jaakkonen et al, 2017). Despite the wide-scale distribution of EHEC in all types of foods, it is primarily transmitted through cattle and so raw or undercooked minced or ground beef (Centers for Disease Control and Prevention, 1997;King et al, 2014;Xiong et al, 2014), but milk and dairy products including especially raw or inadequately pasteurized milk (Kumar et al, 2013;Goh et al, 2002;Allerberger et al, 2001), yoghurt (Morgan et al, 1993), and raw-milk cheeses (Gaulin et al, 2012;Honish et al, 2005) have also been highly susceptible to contamination by EHEC. A multistate outbreak occurred in the US in 2014 (Kraft et al, 2017) and the outbreak in France in 2011 (King et al, 2014) were both linked to EHEC contamination in ground beefs.…”

“…OMS, which is based on the specific binding of the target pathogen to the hydrophobic and magnetized surface of the uniform micro-sized beads with adsorbed antibodies, has been used for separation and concentration of the target pathogen from foods containing competitive microflora and inhibitors by shortening the time of enrichment and removing impurities (Mercanoglu & Aytac, 2006). Therefore, the integration of OMS method is used to improve the microbial analysis performance, providing concentration of the target pathogen, reducing the total analysis time required for the enrichment steps and the limits of detection, as well as eliminating the complex matrix effect of food samples and PCR inhibitors (Luo et al, 2017;Xiong et al, 2014).…”

The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7

“…Serological classification is used for the detection of E. coli O157:H7 mainly by using a monoclonal antibody to establish an enzyme-linked immunosorbent assay (ELISA) and the latex coagulation experiment [4]. Immunologic-based biosensor technologies, such as amperometricimmuno sensors [4,5,6,7], electrochemical detection [8,9], piezoelectric biosensors [10,11], electrical impedance biosensors [12], and immunomagnetic biosensors [13] have been widely applied for the detection of bacteria. With the rapid development in molecular biology, the polymerase chain reaction (PCR) has been widely used to evaluate E. coli O157:H7 without environmental influences [14,15,16].…”