Development of an immunomagnetic bead-based time-resolved fluorescence immunoassay for rapid determination of levels of carcinoembryonic antigen in human serum

Fluorescent silver nanoparticle based highly sensitive immunoassay for early detection of HIV infection

“…22,23 Fluorescence methods may also be multiplexed, thereby using lower volumes of reagents and decreasing the assay time. 24 The ability to spectroscopically detect multiple analytes in a single well assay is not without its challenges.…”

Reprint of "Cathode photoelectrochemical immunoassay based on analyte- induced formation of exciton trapping for carcinoembryonic antigen detection"

“…The average standard deviation gained from 5 independent experiments for all of the 8 concentrations was 2.9%, indicating good reproducibility and reliability of this method. The linear response range was wider than those based on fluorescence resonance energy transfer (0.257-12.9 ng/mL) [35], electrochemical immunoassay (0.01-100 ng/mL) [36], time-resolved fluoroimmunoassay (1-1000 ng/mL) [37], chemiluminescent immunoassay (5.0-250 ng/mL) [38], laser-induced fluorescence (0.7-80 ng/mL) [39], and gold nanoparticle immunoassay (0.10-80 ng/mL) [40] detections. The LOD was also lower than those of electrochemical immunoassay (0.02 ng/mL) [34], time-resolved fluoroimmunoassay (0.5 ng/mL) [37], chemiluminescent immunoassay (0.61 ng/L) [38], and gold nanoparticle immunoassay (0.04 ng/mL) [40] detections.…”

Development of a time-resolved fluoroimmunoassay for Epstein–Barr virus viral capsid antigen IgA antibody in human serum

“…Time-resolved fluoroimmunoassay (TRFIA) has been rapidly developed and is widely used in clinical disease diagnostics, and offers high sensitivity and specificity, a wide dynamic range and better reproducibility compared with other diagnostic methods (Hou et al, 2012). In this study, we aimed to develop and evaluate an accurate and sensitive indirect TRFIA for the quantitative determination of IgA antibodies to VCA in human serum.…”