Development of an Immunofiltration‐Based Antigen‐Detection Assay for Rapid Diagnosis of Ebola Virus Infection

Lucht, A; Formenty, Pierre; Feldmann, Heinz; Götz, Marion G.; Leroy, Eric M.; Bataboukila, Pierre; Grolla, Allen; Feldmann, Friederike; Wittmann, Tatiana; Campbell, Patricia T.; Atsangandoko, C.; Boumandoki, Paul; Finke, Ernst-Jürgen; Miethe, Peter; Becker, Stephan; Grunow, Roland

doi:10.1086/520593

Cited by 39 publications

(27 citation statements)

References 22 publications

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“…This H5N1 AC-ELISA shows enough specificity and sensitivity for detection of multiple clades of H5N1 HPAI virus strains. This study suggests that the H5N1 AC-ELISA based on concurrent use of H5-and N1-specific MAbs described here could be formatted as a rapid field test based on dot ELISA, immunofiltration, or electrochemical biosensor technologies (14), which would greatly facilitate field investigations.…”

Section: Discussionmentioning

confidence: 93%

Rapid Detection of H5N1 Subtype Influenza Viruses by Antigen Capture Enzyme-Linked Immunosorbent Assay Using H5- and N1-Specific Monoclonal Antibodies

Qian

et al. 2009

Clin Vaccine Immunol

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Highly pathogenic avian influenza (HPAI) virus of the H5N1 subtype has caused devastating damage to poultry flocks and sporadic human H5N1 infections. There is concern that this virus subtype may gain transmissibility and become pandemic. Rapid diagnosis and surveillance for H5N1 subtype viruses are critical for the control of H5N1 infection. In this study, we report a robust antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) based on H5-and N1-specific monoclonal antibodies (

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Section: Discussionmentioning

confidence: 93%

Rapid Detection of H5N1 Subtype Influenza Viruses by Antigen Capture Enzyme-Linked Immunosorbent Assay Using H5- and N1-Specific Monoclonal Antibodies

Qian

et al. 2009

Clin Vaccine Immunol

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“…Magnetic immunodetection combined with immunofiltration has numerous advantages over classic immunological assays, which remain the assays that are the most widely used for virus detection in plants. The speed, sensitivity, specificity, and convenience of immunofiltration-based assays have already been compared favorably to those of established detection techniques for the human pathogen Ebola virus (47). Typically, double-antibody sandwich ELISA protocols have detection limits of 10 to 500 ng/ml (36,37).…”

Section: Discussionmentioning

confidence: 99%

“…Immunofiltration was carried out using ABICAP immunofiltration columns (44)(45)(46)(47), purchased from Senova GmbH (Weimar, Germany), with sintered polyethylene filters with a pore diameter of 50 m. The columns were equilibrated in 96% (vol/vol) ethanol at 8 ϫ 10 3 Pa in a desiccator for 20 min, and subsequent steps were carried out in flowthrough mode. The degassed columns were first washed with 750 l ethanol-water (50/50), followed by 750 l water and 750 l immobilization buffer [15 mM Na 2 (CO 3 ), 35 mM NaHCO 3 , pH 9.6].…”

Section: Methodsmentioning

confidence: 99%

Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses

Rettcher

Jungk

Kühn

et al. 2015

Appl Environ Microbiol

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c Plant pathogens cause major economic losses in the agricultural industry because late detection delays the implementation of measures that can prevent their dissemination. Sensitive and robust procedures for the rapid detection of plant pathogens are therefore required to reduce yield losses and the use of expensive, environmentally damaging chemicals. Here we describe a simple and portable system for the rapid detection of viral pathogens in infected plants based on immunofiltration, subsequent magnetic detection, and the quantification of magnetically labeled virus particles. Grapevine fanleaf virus (GFLV) was chosen as a model pathogen. Monoclonal antibodies recognizing the GFLV capsid protein were immobilized onto immunofiltration columns, and the same antibodies were linked to magnetic nanoparticles. GFLV was quantified by immunofiltration with magnetic labeling in a double-antibody sandwich configuration. A magnetic frequency mixing technique, in which a two-frequency magnetic excitation field was used to induce a sum frequency signal in the resonant detection coil, corresponding to the virus concentration within the immunofiltration column, was used for high-sensitivity quantification. We were able to measure GFLV concentrations in the range of 6 ng/ml to 20 g/ml in less than 30 min. The magnetic immunoassay could also be adapted to detect other plant viruses, including Potato virus X and Tobacco mosaic virus, with detection limits of 2 to 60 ng/ml.

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“…Columns were constructed by using the method of Lucht et al (24). Briefly, polyethylene filters (frits) (2.5 by 5 mm; pore size, 40 m) were degassed in ethanol (96%, vol/vol), which was followed by three washes in 50% ethanol.…”

Section: Methodsmentioning

confidence: 99%

Rapid Affinity Immunochromatography Column-Based Tests for Sensitive Detection of Clostridium botulinum Neurotoxins and Escherichia coli O157

Brunt

Webb

Peck

2010

Appl Environ Microbiol

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Existing methods for detection of food-borne pathogens and their toxins are frequently time-consuming, require specialized equipment, and involve lengthy culture procedures and/or animal testing and are thus unsuitable for a rapid response to an emergency public health situation. A series of simple and rapid affinity immunochromatography column (AICC) assays were developed to detect Clostridium botulinum neurotoxin types A, B, E, and F and Escherichia coli O157 in food matrices. Specifically, for milk, grape juice with peach juice, and bottled water, the detection limit for the botulinum neurotoxin type A complex was 0.5 ng. Use of this method with a 10-ml sample would therefore result in a detection limit of 50 pg ml ؊l . Thus, this assay is approximately 2 orders of magnitude more sensitive than a comparable lateral-flow assay. For botulinum neurotoxin complex types B, E, and F, the minimum detection limit was 5 ng to 50 ng. Sensitive detection of E. coli O157 was achieved, and the detection limit was 500 cells. The AICC test was also shown to be specific, rapid, and user friendly. This test takes only 15 to 30 min to complete without any specialized equipment and thus is suitable for use in the field. It has the potential to replace existing methods for presumptive detection of botulinum neurotoxin types A, B, E, and F and E. coli O157 in contaminated matrices without a requirement for preenrichment.

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Development of an Immunofiltration‐Based Antigen‐Detection Assay for Rapid Diagnosis of Ebola Virus Infection

Cited by 39 publications

References 22 publications

Rapid Detection of H5N1 Subtype Influenza Viruses by Antigen Capture Enzyme-Linked Immunosorbent Assay Using H5- and N1-Specific Monoclonal Antibodies

Rapid Detection of H5N1 Subtype Influenza Viruses by Antigen Capture Enzyme-Linked Immunosorbent Assay Using H5- and N1-Specific Monoclonal Antibodies

Simple and Portable Magnetic Immunoassay for Rapid Detection and Sensitive Quantification of Plant Viruses

Rapid Affinity Immunochromatography Column-Based Tests for Sensitive Detection of Clostridium botulinum Neurotoxins and Escherichia coli O157

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