Development of an immunochromatographic lateral flow strip test for the rapid detection of diclofenac in medicinal wine

Abstract

A rapid immunochromatographic lateral flow strip test (ILFST) of competitive format for the detection of diclofenac (DCF) in medicinal wine samples was developed. The half-inhibitory concentration (IC 50) values of the ILFST for DCF and the limit of detection (LOD) in DCF-spiked samples were 0.66 and 0.15 ng/mL, respectively. Recoveries of DCF from spiked medicinal wine samples were from 84.4% to 85.9% within an assay (intra-assay) and from 83.7% to 88.4% between assays (inter-assay). The coefficients of variation (CV) for intra-assay and inter-assay were 4.10-7.56% and 4.71-8.94%, respectively. These results indicated that the ILFST was highly sensitive and reproducible. The same authentic medicinal wine samples were tested by both ILFST and high-performance liquid chromatography (HPLC) respectively. The results showed no significant differences between the two methods. Therefore, the ILFST can be widely used as a convenient tool for both qualitative and quantitative detection of DCF residues in medicinal wine samples.

Research progress in methods for detecting neutralizing antibodies against SARS-CoV-2

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Rapid and sensitive detection of ochratoxin A in rice flour using a fluorescent microsphere immunochromatographic test strip assay

“…In order to detect OTA in food and raw materials, many methods have been widely used in market factories, including high-performance liquid chromatography (HPLC) (Turner et al, 2015;Yang et al, 2020), liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Kai et al, 2017) and Raman spectroscopy (Li et al, 2017), sensitive sensor (He et al, 2019) and enzyme-linked immunosorbent assay (ELISA) (Elaridi et al, 2019). With the advent of rapidly ecvolving time-resolved fluorometry (TR-FIA) (Liu et al, 2019), there have been many fluorescent microsphere (FM) immunoassay experiments that have been performed using fluorescent microsphere beads as probe (Zhang et al, 2016;.…”

Design and Analytical Performances of a Diclofenac Biosensor for Water Resources Monitoring

“…Chromatographic methods are routinely used to quantify DCF residues in surface and ground waters. , Alternatively, label-based immunochemical assays allow to quantify DCF in water samples with sensitivities from the μg/L down to the sub-ng/L range, depending on the detection method. − Although these methods are selective and sensitive, they are not easily amenable to on-site detection. In this context, various biosensing setups have been implemented using antibodies, aptamers, , or even whole cells − as bioreceptors coupled to electrochemical − or optical , signal transduction.…”