Development of an immunochromatographic assay for the rapid detection of AAC(6′)-Iae-producing multidrug-resistant Pseudomonas aeruginosa

Kitao, Tomoe; Miyoshi‐Akiyama, Tohru; Shimada, Kayo; Tanaka, Masashi; Narahara, Kenji; Saito, Noriko; Kirikae, Teruo

doi:10.1093/jac/dkq148

Cited by 28 publications

(15 citation statements)

References 9 publications

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“…Quick Chaser Iae IMP® (Mizuho Medy Co. Ltd, Tokyo, Japan) was used to detect the production of IMP-type metallo-β-lactamase and aminoglycoside 6'-N-acetyl-transference (AAC(6′)-Iae), which is an aminoglycoside-modifying enzyme [8,9]. …”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo Pharmacodynamics of Colistin and Aztreonam Alone and in Combination against Multidrug-Resistant Pseudomonas aeruginosa

Yamagishi

Hagihara²,

Kato³

et al. 2016

Chemotherapy

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Background: Reports of Pseudomonas aeruginosa with high antimicrobial resistance have steadily emerged, threatening the utility of a mainstay in antipseudomonal therapy. This study evaluated the antimicrobial activities of various combination therapies against P. aeruginosa with high antimicrobial resistance, including multidrug-resistant P. aeruginosa (MDRP) using an in vitro and in vivo study. Methods: We evaluated 24 combination therapies, including colistin, aztreonam, meropenem, ceftazidime, ciprofloxacin, amikacin, rifampicin, arbekacin and piperacillin against 15 MDRP isolates detected at Aichi Medical University Hospital with the break-point checkerboard method. Based on the results of the in vitro study, we evaluated antimicrobial activity against highly antimicrobial-resistant P. aeruginosa with an in vivo murine thigh infection model. Results: The combination regimens including colistin and aztreonam showed higher antimicrobial activity against the 15 MDRP isolates. In the in vivo study, the high-dose colistin monotherapy (16 mg/kg every 12 h) achieved greater log₁₀ CFU changes than the normal-dose colistin regimen (8 mg/kg every 12 h) against 5 P. aeruginosa isolates, including 2 MDRP isolates (p < 0.05). Aztreonam monotherapy (400 mg every 8 h) yielded bacterial densities similar to untreated control mice for the MDRP isolate evaluated. The combination therapy with a higher dose of colistin had superior antimicrobial activity against 5 P. aeruginosa with colistin (MIC 0.5 μg/ml) and aztreonam (MIC ≥128 μg/ml) than colistin monotherapy. Conclusion: The data suggest that the combination treatment of colistin and aztreonam could be the most useful for treating highly resistant P. aeruginosa with a higher susceptibility to colistin, including MDRP infections.

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Section: Methodsmentioning

confidence: 99%

In vitro and in vivo Pharmacodynamics of Colistin and Aztreonam Alone and in Combination against Multidrug-Resistant Pseudomonas aeruginosa

Yamagishi

Hagihara²,

Kato³

et al. 2016

Chemotherapy

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“…NCGM1588 showed high levels of AMK resistance. In particular, it showed high levels of ABK resistance, whereas the representative epidemic strain of MDR P. aeruginosa IMCJ2.S1 in Japan was susceptible to ABK (9,15). The serotype of NCGM1588 was O7, and the MLST was ST560.…”

Section: Resultsmentioning

confidence: 99%

Novel 6′- N -Aminoglycoside Acetyltransferase AAC(6′)-Iaj from a Clinical Isolate of Pseudomonas aeruginosa

Tada

Miyoshi‐Akiyama

Shimada

et al. 2013

Antimicrob Agents Chemother

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bPseudomonas aeruginosa NCGM1588 has a novel chromosomal class 1 integron, In151, which includes the aac(6=)-Iaj gene. The encoded protein, AAC(6=)-Iaj, was found to consist of 184 amino acids, with 70% identity to AAC(6=)-Ia. Escherichia coli transformed with a plasmid containing the aac(6=)-Iaj gene acquired resistance to all aminoglycosides tested except gentamicin. Of note, aac(6=)-Iaj contributed to the resistance to arbekacin. Thin-layer chromatography revealed that AAC(6=)-Iaj acetylated all aminoglycosides tested except gentamicin. These findings indicated that AAC(6=)-Iaj is a functional acetyltransferase that modifies the amino groups at the 6= positions of aminoglycosides and contributes to aminoglycoside resistance of P. aeruginosa NCGM1588, including arbekacin.

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“…Approximately 70% of MDR P. aeruginosa isolates in Japan produced AAC(6′)-Iae (Kitao et al, 2012). We first developed an immunochromatographic assay to detect AAC(6′)-Iae (Kitao et al, 2010). In this study, an immunochromatographic assay using mAbs to AAC(6′)-Ib was developed.…”

mentioning

confidence: 99%

“…Recombinant His-tagged AAC(6′)-Ib, AAC(6′)-Iae (Sekiguchi et al, 2005), AAC(6′)-Iaf (Kitao et al, 2009), and AAC(6′)-Iaj (Tada et al, unpublished results), purified by Protein G Sepharose 4 Fast Flow (GE Healthcare Bio-Science, Tokyo, Japan) were prepared as described previously (Kitao et al, 2010). Anti-AAC(6′)-Ib monoclonal antibodies (mAbs) were prepared as described previously (Kitao et al, 2010). The animal experiments were approved by the Ethics Committee for Animal Experiments at NCGM (approval number: NCGM23-C-1).…”

mentioning

confidence: 99%

Development of an immunochromatographic assay for rapid detection of AAC(6′)-Ib-producing Pseudomonas aeruginosa

Tada

Miyoshi‐Akiyama

Tanaka

et al. 2012

Journal of Microbiological Methods

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Development of an immunochromatographic assay for the rapid detection of AAC(6′)-Iae-producing multidrug-resistant Pseudomonas aeruginosa

Abstract: The developed assay is an easy-to-use and reliable detection method for AAC(6')-Iae-producing MDR P. aeruginosa. This approach may be applicable for screening and investigation of antibiotic-resistant bacteria as an alternative to PCR analysis.

Cited by 28 publications

References 9 publications

In vitro and in vivo Pharmacodynamics of Colistin and Aztreonam Alone and in Combination against Multidrug-Resistant Pseudomonas aeruginosa

In vitro and in vivo Pharmacodynamics of Colistin and Aztreonam Alone and in Combination against Multidrug-Resistant Pseudomonas aeruginosa

Novel 6′- N -Aminoglycoside Acetyltransferase AAC(6′)-Iaj from a Clinical Isolate of Pseudomonas aeruginosa

Development of an immunochromatographic assay for rapid detection of AAC(6′)-Ib-producing Pseudomonas aeruginosa

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