Development of an immunoaffinity column for the highly sensitive analysis of bisphenol A in 14 kinds of foodstuffs using ultra-high-performance liquid chromatography tandem mass spectrometry

Yao, Kai; Wen, Kai; Shan, Wenchong; Xie, Su‐Yuan; Peng, Tao; Wang, Jianyi; Jiang, Haiyang; Lei, Lei

doi:10.1016/j.jchromb.2018.02.013

Cited by 27 publications

(12 citation statements)

References 26 publications

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“…All samples were divided into three broad categories: plant-derived foods (including cereals and cereal products, legume and related products, potatoes and potato products, vegetables and vegetable products, fruits and fruit products, and sugar and sugar products), animal-derived foods (including meats and meat products, eggs and egg products, aquatic foods and aquatic food products, and milk and dairy products), and beverages (including beverages and water and alcohol beverages). The sample preparation methods were based on our previous work [ 30 , 31 ] with some modifications.…”

Section: Methodsmentioning

confidence: 99%

Bisphenol A and Its Analogues in Chinese Total Diets: Contaminated Levels and Risk Assessment

Yao

Zhang

Yin

et al. 2020

Oxidative Medicine and Cellular Longevity

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Bisphenol A (BPA) and its analogues (BPs) are suspected posing potential endocrine disrupting properties. They might migrate into foodstuffs through food packaging materials or contaminated water and soil. Dietary exposure is of paramount importance way for human health. European Food Safety Authority (EFSA) lowered the value of tolerable daily intake (TDI) from 50 μg/kg bw/day (d) to a temporary (t) TDI (t-TDI) of 4 μg/kg bw/d. In this study, the Chinese total dietary samples were analyzed for assessing the exposure risk of BPs by diets. BPA, bisphenol F (BPF), bisphenol S (BPS), and bisphenol AF (BPAF) were found in 12 kinds of food samples except for bisphenol B (BPB). A deterministic approach was used to calculate the dietary intakes of 4 kinds of compounds. For different age and gender groups, the exposure levels of BPA (178.440-403.672 ng/kg bw/d) was the highest, followed by BPS (21.372-52.112 ng/kg bw/d), BPF (20.641-50.507 ng/kg bw/d), and BPAF (0.434-1.210 ng/kg bw/d). Based on the t-TDI set by EFSA (4 μg/kg bw/d for BPA), the BPs through dietary intake pose low risks on the Chinese general population even summarization exposure levels of different BPs. However, human can be exposed to multiple endocrine disrupting chemicals rather than BPs alone; combined exposure risks should be further considered.

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Section: Methodsmentioning

confidence: 99%

Bisphenol A and Its Analogues in Chinese Total Diets: Contaminated Levels and Risk Assessment

Yao

Zhang

Yin

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…Suitable elution conditions serve to dissociate the analyte–antibody interactions and return the target analytes to the mobile phase . MeOH or MeOH/water modified mixtures have typically been used for analyte elution in the IAC cleanup process. , The effects of the percentage of MeOH in the eluent and the total volume of eluent used on the recoveries of TCC and TCS have been evaluated to identify the optimal conditions. As shown in Figure B, with increasing MeOH concentration, the recoveries of TCC and TCS increased from 58.5% to 75.6% and from 53.2% to 106.9%, respectively.…”

Section: Resultsmentioning

confidence: 99%

An Immunoaffinity Purification Method for the Simultaneous Analysis of Triclocarban and Triclosan in Foodstuffs by Liquid Chromatography Tandem Mass Spectrometry

Yao

Wen

Shan

et al. 2019

J. Agric. Food Chem.

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Triclocarban (TCC) and triclosan (TCS) have been simultaneously detected in five kinds of foodstuffs using an immunoaffinity purification method coupled with ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) for the first time. Two highly specific monoclonal antibodies against TCC and TCS were produced and coupled to N-hydroxysuccinimide-activated Sepharose 6B gel to prepare the immunosorbent. Under the optimal conditions, mean recoveries from spiked samples by the IAC-UHPLC-MS/MS method were 70.1−92.8% for TCC and 76.6−102.5% for TCS. Intraday relative standard deviations were below 14.5%. The limits of quantification (LOQs) of TCC were 1 ng/L for beverage samples and 0.01−0.02 μg/kg for food samples. The LOQs of TCS were 0.03 μg/L for beverage samples and 0.2−0.3 μg/kg for food samples. The applicability of the method has been proven by analyzing TCC and TCS in different samples from supermarkets in Beijing. The proposed method is sufficiently sensitive and reliable for monitoring trace concentrations of TCC and TCS in food samples.

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“…Immunoaffinity chromatography is a specific type of affinity chromatography, which is based on the certain and firm antigen-antibody interactions. Before the purification process, antigens or antibodies are covalently immobilized on support materials such as agarose, cellulose, acrylamide or chromatographic resins [25][26][27][28][29][30][31]. However, due to the low binding constant and large molecular size of hIgM, macroporous support materials like monoliths are preferably used during hIgM purification [17,21,22,25].…”

Section: Introductionmentioning

confidence: 99%

Poly(Hydroxyethyl Methacrylate) Immunoaffinity Cryogel Column for the Purification of Human Immunoglobulin M

Bakhshpour

Topçu

Bereli

et al. 2020

Gels

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Human immunoglobulin M (hIgM) antibodies are considered as hopeful tools for diseases therapy. Therefore, chromatography approaches are used to purify hIgM with a single step. In this study, we prepared a poly(hydroxyethyl methacrylate) based immunoaffinity p(HEMA-I) cryogel column by using cyanamide to immobilize antihuman immunoglobulin on the p(HEMA) cryogel for purification of hIgM in aqueous solution and artificial human plasma. The characterization of the p(HEMA) cryogel column was performed by using a scanning electron microscope (SEM), micro-computerized tomography (µ-CT), Fourier transform infrared spectroscopy (FTIR), swelling degree and macro-porosity. Further, the optimizations of various parameters were performed such as, pH, ionic strength, temperature and concentration of hIgM in aqueous solutions. In addition, the Langmuir adsorption model was supported by experimental results. Maximum adsorbed amount of hIgM corresponded to 11.1 mg/g at pH 5.75 [morpholino ethanesulfonic acid (MES buffer)]. Our results indicated that the p(HEMA-I) cryogel column can be reused at least 10 times without significant loss in adsorption capacity. As a natural source, artificial human plasma was selected for hIgM adsorption and the purity of hIgM was evaluated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

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Development of an immunoaffinity column for the highly sensitive analysis of bisphenol A in 14 kinds of foodstuffs using ultra-high-performance liquid chromatography tandem mass spectrometry

Cited by 27 publications

References 26 publications

Bisphenol A and Its Analogues in Chinese Total Diets: Contaminated Levels and Risk Assessment

Bisphenol A and Its Analogues in Chinese Total Diets: Contaminated Levels and Risk Assessment

An Immunoaffinity Purification Method for the Simultaneous Analysis of Triclocarban and Triclosan in Foodstuffs by Liquid Chromatography Tandem Mass Spectrometry

Poly(Hydroxyethyl Methacrylate) Immunoaffinity Cryogel Column for the Purification of Human Immunoglobulin M

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