Development of an
            <i>In Vitro</i>
            Model of the Gut Microbiota Enriched in Mucus-Adhering Bacteria

Calvigioni, Marco; Panattoni, Adelaide; Biagini, Francesco; Donati, Leonardo; Mazzantini, Diletta; Massimino, Mariacristina; Daddi, Costanza; Celandroni, Francesco; Vozzi, Giovanni; Ghelardi, Emilia

doi:10.1128/spectrum.00336-23

Cited by 5 publications

(6 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Caco-2 and HT-29 cells were cultured on hydrogel scaffolds to demonstrate that mucus was a protective factor against bacterial colonization by adherent-invasive E. coli (AIEC) [ 26 ]. Raw and mucin-coated 3D gelatin electrospun membranes were successfully used as scaffolds to culture the human gut microbiota in vitro and reproduce the three-dimensional architecture of the intestinal mucosa and the arrangement of bacteria on it [ 27 , 28 , 29 ]. Cells assembled in organoids were also recently used in gut microbiota research.…”

Section: Animal Models or In Vitro Models For Gut Microbiota Research...mentioning

confidence: 99%

Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

Calvigioni,

Mazzantini,

Celandroni

et al. 2023

Microorganisms

Self Cite

View full text Add to dashboard Cite

Examining the interplay between intestinal pathogens and the gut microbiota is crucial to fully comprehend the pathogenic role of enteropathogens and their broader impact on human health. Valid alternatives to human studies have been introduced in laboratory practice to evaluate the effects of infectious agents on the gut microbiota, thereby exploring their translational implications in intestinal functionality and overall health. Different animal species are currently used as valuable models for intestinal infections. In addition, considering the recent advances in bioengineering, futuristic in vitro models resembling the intestinal environment are also available for this purpose. In this review, the impact of the main human enteropathogens (i.e., Clostridioides difficile, Campylobacter jejuni, diarrheagenic Escherichia coli, non-typhoidal Salmonella enterica, Shigella flexneri and Shigella sonnei, Vibrio cholerae, and Bacillus cereus) on intestinal microbial communities is summarized, with specific emphasis on results derived from investigations employing animal and in vitro models.

show abstract

Section: Animal Models or In Vitro Models For Gut Microbiota Research...mentioning

confidence: 99%

Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

Calvigioni,

Mazzantini,

Celandroni

et al. 2023

Microorganisms

Self Cite

View full text Add to dashboard Cite

show abstract

“…As previously reported, microbial DNAs were extracted from EGM scaffolds using the phenol–chloroform method [ 10 ]. Extracted DNAs were quantified using NanoDrop Lite Spectrophotometer (Thermo Fisher Scientific) and normalized to a standard 5 ng DNA/µL concentration.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, a previously validated three-dimensional (3D) in vitro model of the human gut microbiota [ 8 , 9 , 10 ] was used. The model is based on electrospun reticular gelatin scaffolds suitable for enhancing microbial adhesion and biofilm formation and preserving the richness and biodiversity of the in vitro-cultured gut microbes, as reported by Biagini et al [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…The model is based on electrospun reticular gelatin scaffolds suitable for enhancing microbial adhesion and biofilm formation and preserving the richness and biodiversity of the in vitro-cultured gut microbes, as reported by Biagini et al [ 8 , 9 ]. It was also shown that adding a mucin coat to the electrospun structures selects intestinal microorganisms able to adhere to mucins following adaptative behaviors in the presence of mucus [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Impact of Bacillus cereus on the Human Gut Microbiota in a 3D In Vitro Model

et al. 2023

Self Cite

View full text Add to dashboard Cite

In vitro models for culturing complex microbial communities are progressively being used to study the effects of different factors on the modeling of in vitro-cultured microorganisms. In previous work, we validated a 3D in vitro model of the human gut microbiota based on electrospun gelatin scaffolds covered with mucins. The aim of this study was to evaluate the effect of Bacillus cereus, a pathogen responsible for food poisoning diseases in humans, on the gut microbiota grown in the model. Real-time quantitative PCR and 16S ribosomal RNA-gene sequencing were performed to obtain information on microbiota composition after introducing B. cereus ATCC 14579 vegetative cells or culture supernatants. The adhesion of B. cereus to intestinal mucins was also tested. The presence of B. cereus induced important modifications in the intestinal communities. Notably, levels of Proteobacteria (particularly Escherichia coli), Lactobacillus, and Akkermansia were reduced, while abundances of Bifidobacterium and Mitsuokella increased. In addition, B. cereus was able to adhere to mucins. The results obtained from our in vitro model stress the hypothesis that B. cereus is able to colonize the intestinal mucosa by stably adhering to mucins and impacting intestinal microbial communities as an additional pathogenetic mechanism during gastrointestinal infection.

show abstract

“…Since their original inception in 2016, microfluidic gut-on-chip platforms have emerged as powerful research tools to partially deconstruct the complexity that operates in vivo and address the role of phenomena such as varying mechanical forces, fluid flow, and oxygenation conditions while capturing other dynamic aspects of the gut's physiological context by incorporating human cells and microbes [24 ▪▪ ]. While these approaches have shown to be powerful and robust, they are complex to build and operate and rely on synthetic polymers such as polydimethylsiloxane (PDMS), which is prone to adsorption by the chemical compounds present.…”

Section: In Vitro Modelsmentioning

confidence: 99%

The Rosetta Stone of interactions of mucosa and associated bacteria in the gastrointestinal tract

Berberolli,

Wu,

Goycoolea

2023

Current Opinion in Gastroenterology

View full text Add to dashboard Cite

Purpose of review Gut microbiota–mucosa–epithelial cells co-exist in an intricate three-way relationship that underpins gut homeostasis, and ultimately influences health and disease conditions. The O-glycans of mucin glycoproteins have been uncovered as a centrepiece of this system, although understanding the phenomena at play at the molecular level has been challenging and subject to significant traction over the last years. The purpose of this review is to discuss the recent advances in the phenomena that mediate microbiota and mucus multidirectional interactions in the human gut. Recent findings The mucus biosynthesis and degradation by both commensal and pathogenic bacteria is under tight regulation and involves hundreds of carbohydrate-active enzymes (CAZy) and transporters. The fucosylation of O-glycans from mucin-2 seems to dictate binding by pathogenic species and to influence their virulence. Less clear is the influence of O-glycans in quorum sensing and biofilm formation. We have reviewed the advances in the in vitro models available to recreate the phenomena that capture the physiological context of the intestinal environment, emphasising models that include mucus and other aspects relevant to the physiological context. Summary The recent findings highlight the importance of merging advances in analytical (glycans analysis) and omics techniques along with original robust in vitro models that enable to deconstruct part of the high complexity of the living gut and expand our understanding of the microbes-mucosa relationships and their significance in health and disease.

show abstract

Development of an In Vitro Model of the Gut Microbiota Enriched in Mucus-Adhering Bacteria

Cited by 5 publications

References 51 publications

Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

Impact of Bacillus cereus on the Human Gut Microbiota in a 3D In Vitro Model

The Rosetta Stone of interactions of mucosa and associated bacteria in the gastrointestinal tract

Contact Info

Product

Resources

About