Development of an hydrophobic fluoro-silica surface for studying homotypic cancer cell aggregation–disaggregation as a single dynamic process in vitro

Nicklin, Matthew; Rees, Robert C.; Pockley, A. Graham; Perry, Carole C.

doi:10.1039/c4bm00194j

Cited by 8 publications

(15 citation statements)

References 49 publications

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“…Cell culture vessels were also modified into hydrophobic fluoro-silica (FS) surface resulting in low adherence and transient aggregation of breast cancer cells followed by disaggregation. This method was suggested as a tool for study metastatic events where cancer cells disassociate from the tumor, increase their mobility and settle in new tissues [99]. Silica and cell culture media were also mixed to make hydrogels which were tested as a 3D platform to culture different types of mammalian cells including cancer cells.…”

Section: Gelatin Fibrin Alginate and Agarosementioning

confidence: 99%

Cancer Stem Cell Microenvironment Models with Biomaterial Scaffolds In Vitro

et al. 2020

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Defined by its potential for self-renewal, differentiation and tumorigenicity, cancer stem cells (CSCs) are considered responsible for drug resistance and relapse. To understand the behavior of CSC, the effects of the microenvironment in each tissue are a matter of great concerns for scientists in cancer biology. However, there are many complicated obstacles in the mimicking the microenvironment of CSCs even with current advanced technology. In this context, novel biomaterials have widely been assessed as in vitro platforms for their ability to mimic cancer microenvironment. These efforts should be successful to identify and characterize various CSCs specific in each type of cancer. Therefore, extracellular matrix scaffolds made of biomaterial will modulate the interactions and facilitate the investigation of CSC associated with biological phenomena simplifying the complexity of the microenvironment. In this review, we summarize latest advances in biomaterial scaffolds, which are exploited to mimic CSC microenvironment, and their chemical and biological requirements with discussion. The discussion includes the possible effects on both cells in tumors and microenvironment to propose what the critical factors are in controlling the CSC microenvironment focusing the future investigation. Our insights on their availability in drug screening will also follow the discussion.

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Section: Gelatin Fibrin Alginate and Agarosementioning

confidence: 99%

Cancer Stem Cell Microenvironment Models with Biomaterial Scaffolds In Vitro

et al. 2020

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“…88,89 Cell responses such as surface adhesion and subsequent differentiation are the result of interactions between the surface of the material and the macromolecules that adsorb to the surface and are influenced by the surrounding environment or "niche". 84,89 The nature of the ECM reflects the broad range of biomolecules, such as proteoglycans, polysaccharides, fibrous proteins such as collagens, and adhesion proteins such as fibronectin and vitronectin, that are available. 90 Macromolecules such as proteins and carbohydrates are important constituents of most biological media and will adsorb to the surface over time, displacing bound water, ions, and other smaller species.…”

Section: Defining a Model Of Cell Surfacementioning

confidence: 99%

“…Adhesion is not a guaranteed outcome of cell-protein-surface interaction, as stable attachment cannot occur when surfaces cannot support CAMs 84,85 . Other cell adhesion processes such as anchoring, and the establishment of tight and gap junctions are associated with cell-cell rather than cell-surface adhesion 86 .…”

Section: Cellular Adhesion (C)mentioning

confidence: 99%

“…supply of CAMs in the media 88,89 . Cell responses such as surface adhesion and subsequent differentiation are the result of interactions between the surface of the material and the macromolecules that adsorb to the surface, and are influenced by the surrounding environment or 'niche' 84,89 . The nature of the ECM reflects the broad range of biomolecules such as proteoglycans, polysaccharides, fibrous proteins like collagens and adhesion proteins such as fibronectin and vitronectin that are available 90 .…”

Section: Extracellular Matrix; Biological Surface Modification (D)mentioning

confidence: 99%

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The Importance and Clinical Relevance of Surfaces in Tissue Culture

Hickman

Boocock

Pockley

et al. 2016

ACS Biomater. Sci. Eng.

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Cell and tissue culture has evolved from the use of simple glassware for the propagation of cells and tissues into a comprehensive platform for interrogating complex biological systems, directing cell fate and deriving products with clinical and therapeutic value.However, despite significant advances, current in vitro culture approaches remain limited in their capacity to model the clinical/biological complexities of disease, in part at least due to the deficiencies of existing culture materials. The challenge is therefore to identify innovative materials-based solutions that have greater control over cells in vitro, while better representing biological systems in vivo. Such platforms would be suitable for biomarker discovery and tissue engineering applications. This review examines the development of tissue culture materials, advances in our understanding of cell-surface interactions and the application of this knowledge towards the development of new approaches for better examining biological events.3 he ability to culture cells and tissues in vitro is a fundamental aspect of modern science. Established early in the twentieth century, notably through the work of Harrison R.G. of John Hopkins University 1 , the ability to culture cells and tissues has markedly improved during the intervening period. The field has progressed from an ability to maintain and culture tissue for extended periods, through the discovery and establishment of immortal cell lines, to today, where tissue engineering is making considerable progress in the production of artificial tissues and organs in vitro [2][3][4] . Key to these successes have been advances in the culture surfaces on which cells and tissues are grown.This review summarizes progress in the development of tissue culture materials, highlights current requirements and existing limitations for in vitro culture, and examines their relevance to clinical questions and our current understanding of tissue culture materials design. Although long-established, current culture materials may not always be appropriate for modelling in vivo conditions, and innovative strategies are therefore required in order to overcome existing limitations. Current Issues with Tissue Culture:Numerous articles have highlighted the drawbacks and limitations of current in vitro culture systems 5,6 . Concerns revolve around deficiencies in the culture systems and the tissue they generate. Although the latter can be linked to the quality of the initial cellular material, contamination and/or poor maintenance of historical cell lines 6,7 , it can also result from deficiencies in the culture systems i.e. not all cell populations are amenable to in vitro culture.Problems are compounded once tissue enters in vitro culture, as derived populations are expected to maintain their in vivo relevance. However, cells naturally adapt to the local environment and T 4 prolonged culture of immortalized cell lines results in a progressive divergence from the parental population 6,8,9 . Although loss or gain of ab...

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“…A large number of tools for 3-D cell culture have been shown (Lin and Chang 2008;Souza et al 2010). Normal human mammary epithelial (MCF-10a) and breast cancer (MCF-7) cells exhibit transient aggregate formation on fluoro-silica surface in a serum-dependent manner (Nicklin et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Different morphologies of human embryonic kidney 293T cells in various types of culture dishes

et al. 2019

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Human embryonic kidney 293T (HEK293T) cells are used in various biological experiments and researches. In this study, we investigated the effect of cell culture environments on morphological and functional properties of HEK293T cells. We used several kinds of dishes made of polystyrene or glass for cell culture, including three types of polystyrene dishes provided from different manufacturers for suspension and adherent cell culture. In addition, we also investigated the effect of culturing on gelatin-coated surfaces on the cell morphology. We found that HEK293T cells aggregated and formed into three-dimensional (3-D) multicellular spheroids (MCS) when non-coated polystyrene dishes were used for suspension culture. In particular, the non-coated polystyrene dish from Sumitomo bakelite is the most remarkable characteristic for 3-D MCS among the polystyrene dishes. On the other hand, HEK293T cells hardly aggregated and formed 3-D MCS on gelatin-coated polystyrene dishes for suspension culture. HEK293T cells adhered on the non-or gelatin-coated polystyrene dish for adherent culture, but they did not form 3-D MCS. HEK293T cells also adhered to non-or gelatin-coated glass dishes and did not form 3-D MCS in serum-free medium. These results suggest that HEK293T cells cultured on non-coated polystyrene dish may be useful for the tool to analyze the characteristics of 3D-MCS.

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Development of an hydrophobic fluoro-silica surface for studying homotypic cancer cell aggregation–disaggregation as a single dynamic process in vitro

Cited by 8 publications

References 49 publications

Cancer Stem Cell Microenvironment Models with Biomaterial Scaffolds In Vitro

Cancer Stem Cell Microenvironment Models with Biomaterial Scaffolds In Vitro

The Importance and Clinical Relevance of Surfaces in Tissue Culture

Different morphologies of human embryonic kidney 293T cells in various types of culture dishes

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