Development of an Extruder for Open Source 3D Bioprinting

Banović, Luka; Vihar, Boštjan

doi:10.5334/joh.6

Cited by 19 publications

(20 citation statements)

References 21 publications

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“…The design includes the electronics required to control the direction of rotation of the peristaltic pump, so the system could be programmed (in G-code) to extract liquid from one vial and transfer it to another. More generally, a cartesian coordinate robot can be used to automate many tasks in the laboratory, such as moving a camera to capture macroscopic images of museum specimens arranged in trays (Blagoderov et al 2012) or positioning an extruder for 3D bioprinting (Banović and Vihar 2018).…”

Section: Reuse Potential and Adaptabilitymentioning

confidence: 99%

A Cartesian Coordinate Robot for Dispensing Fruit Fly Food

Wayland

Landgraf

2018

Journal of Open Hardware

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The fruit fly, Drosophila melanogaster, continues to be one of the most widely used model organisms in biomedical research. Though chosen for its ease of husbandry, maintaining large numbers of stocks of fruit flies, as done by many laboratories, is labour-intensive. One task which lends itself to automation is the production of the vials of food in which the flies are reared. Fly facilities typically have to generate several thousand vials of fly food each week to sustain their fly stocks. The system presented here combines a cartesian coordinate robot with a peristaltic pump. The design of the robot is based on an open hardware CNC (computer numerical control) machine, and uses belt and pulley actuators for the X and Y axes, and a leadscrew actuator for the Z axis. CNC motion and operation of the peristaltic pump are controlled by grbl (gnea 2018), an open source, embedded, G-code parser. Grbl is written in optimized C and runs directly on an Arduino. A Raspberry Pi is used to generate and stream G-code instructions to Grbl. A touch screen on the Raspberry Pi provides a graphical user interface to the system. Whilst the robot was built for the express purpose of filling vials of fly food, it could potentially be used for other liquid handling tasks in the laboratory.

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Section: Reuse Potential and Adaptabilitymentioning

confidence: 99%

A Cartesian Coordinate Robot for Dispensing Fruit Fly Food

Wayland

Landgraf

2018

Journal of Open Hardware

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“…In some cases, the complexity of a living tissue such as vessels existing in the middle of the scaffold or the multi-material 3D bio-printing of a functional 3D bio-fabricated organ causes serious limitations in the 3D bio-printing of such complex microenvironments due to the drawbacks of existing commercial tools and available techniques in 3D bio-printers (19). Also, existing open source developed 3D bio-printing systems have some disadvantages regarding their thermal control mechanism and offer wide range of in exibilities when it comes to more complex applications (20). This paper eliminates some of the limitations of the existing tools and methods by providing an ultra-precise thermal controlling unit equipped by an integrated heating and cooling system embedded on the developed portable multifunctional 3D bio-printing extruder that is able to maintain the temperature stable in the interval of 0-100º C. Therefore, the system is adoptable to a vast spectrum of bio-materials with a different gelation points and characteristics.…”

Section: Introductionmentioning

confidence: 99%

Design and development of open source portable multi-functional three-dimensional bio-printing extrusion system for hydrogel-based scaffolds fabrication

Behrouzi¹,

Zali²,

Muhaddesi³

et al. 2021

Preprint

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Three-dimensional (3D) bio-printing has been shown up as a state of the art and creative technological solution to nowadays tissue engineering and stem cell research challenges toward human tissue regeneration and construction of artificial living organs. Thereby, using hydrogel-based bio-inks to 3D print living microenvironments is a crucial strategy to reconstruct basically functional living scaffolds in order to shape human living organs based on digital 3D computer-aided design (CAD) inputs. The focus of this paper lies on design and development of a portable multi-functional 3D bio-printing extruder for fabrication of hydrogel-based highly complex living tissues using advanced methods invented along this study. The presented article, precisely optimizes the process of fabricating 3D printed scaffolds by redesigning of an integrated gel-extrusion system capable of controlling the adjustability of thermal condition of bio-inks. Also a UV crosslink module is utilized in the bottom of the extruder to cure hydrogel scaffolds consisting of photo-reacting agents to provide a novel bio-printing experience for end users. As a result, the integrated extrusion system is easily portable and compatible with almost any computer numerical control (CNC) machine. Therefore, it could be simply installed on or removed from any CNC machine or fused deposition modeling (FDM) 3D printing system considering that all the control units remain adjustable. The whole system parameters and the performance of tissue fabrication regarding this developed portable multi-functional 3D bio-printing extruder have been tested and practically confirmed. The thermal control system performance has also been simulated using finite element analysis (FEA) and computational fluid dynamics (CFD) methods. Thus, certified documents have been provided and depicted in this paper.

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“…[1][2][3][4] Technologies that enable cell culture in 3D are referred to as 3D bioprinting and include photolithography, magnetic bioprinting, stereolithography, and direct cell extrusion. [5][6][7][8][9][10] The most critical, unmet challenge of 3D bioprinting has been scaling production in order to meet the needs of traditional HTS, which requires hundreds of thousands of wells to be tested for a given campaign. 11 Of the 3D methods currently available, magnetic bioprinting using cell-repellent surface treatment plates from Greiner Bio-One (Monroe, NC) and ultra-lowattachment (ULA) microcavity plates from Corning Inc (Corning, NY).…”

Section: Introductionmentioning

confidence: 99%

Automating a Magnetic 3D Spheroid Model Technology for High-Throughput Screening

et al. 2019

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Affordable and physiologically relevant three-dimensional (3D) cell-based assays used in high-throughput screening (HTS) are on the rise in early drug discovery. These technologies have been aided by the recent adaptation of novel microplate treatments and spheroid culturing techniques. One such technology involves the use of nanoparticle (NanoShuttle-PL) labeled cells and custom magnetic drives to assist in cell aggregation to ensure rapid 3D structure formation after the cells have been dispensed into microtiter plates. Transitioning this technology from a low-throughput manual benchtop application, as previously published by our lab, into a robotically enabled format achieves orders of magnitude greater throughput but required the development of specialized support hardware. This effort included in-house development, fabrication, and testing of ancillary devices that assist robotic handing and high-precision placement of microtiter plates into an incubator embedded with magnetic drives. Utilizing a “rapid prototyping” approach facilitated by cloud-based computer-aided design software, we built the necessary components using hobby-grade 3D printers with turnaround times that rival those of traditional manufacturing/development practices at a substantially reduced cost. This approach culminated in a first-in-class HTS-compatible 3D system in which we have coupled 3D bioprinting to a fully automated HTS robotic platform utilizing our novel magnetic incubator shelf assemblies.

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Development of an Extruder for Open Source 3D Bioprinting

Cited by 19 publications

References 21 publications

A Cartesian Coordinate Robot for Dispensing Fruit Fly Food

A Cartesian Coordinate Robot for Dispensing Fruit Fly Food

Design and development of open source portable multi-functional three-dimensional bio-printing extrusion system for hydrogel-based scaffolds fabrication

Automating a Magnetic 3D Spheroid Model Technology for High-Throughput Screening

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