Development of an enzyme-linked immunosorbent assay for vitellogenin of Morelet's crocodile (Crocodylus moreletii)

Selcer, Kyle W.; Nespoli, Lisa M.; Rainwater, Thomas R.; Finger, Adam G.; Ray, David A.; Smith, Philip; Densmore, Llewellyn D.; McMurry, Scott T.

doi:10.1016/j.cbpc.2005.12.001

Cited by 4 publications

(1 citation statement)

References 56 publications

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“…Crude plasma was diluted in Tris-HCl buffer (Tris-HCl, 25 mM, pH 8.1) and placed in a Q-Sepharose fast flow column (size = 25 mL), then eluted with a linear NaCl gradient in Tris-HCl buffer (varying concentrations of NaCl at pH 8.1 included 0.1, 0.2, 0.3, 0.4 and 0.5 M for fractions 1, 2, 3, 4, and 5, respectively). To obtain only protein fractions, salt was eliminated and dialyzed overnight against diethyl-aminoethyl buffer (DEAE) using a dialysis membrane (Spectra/Por 1) (Spectrum, Houston, TX, USA, cut off 250 kDa) [7].…”

Section: Separation Of Protein From Crocodile Plasmamentioning

confidence: 99%

Siamese crocodile plasma synergizes with ceftazidime against ceftazidime-resistant <i>Enterobacter cloacae</i>

Rojtinnakorn¹,

Temsiripong²,

Pimchan³

et al. 2018

Trop. J. Pharm Res

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Purpose: To evaluate whether Siamese crocodile plasma exhibits antibacterial properties and if it synergizes with ceftazidime against ceftazidime-resistant Enterobacter cloacae (CREnC). Methods: Protein fractions were from crocodile plasma and tested them on CREnC strains. Multiplex polymerase chain reaction (PCR) screening test was performed for extended-spectrum β-lactamase (ESBL) phenotype and AmpC gene. The effects of the antibacterial agents were analyzed using a bacterial suspension standard curve, minimum inhibitory concentration (MIC), Checkerboard assays, viability curves, membrane permeability assays, enzyme assays, and transmission electron microscopy. Results: CREnC strains expressed ESBL-AmpC gene combinations. The MICs of resuspended protein 1 (P1), protein 5 (P5), ceftazidime, cefotaxime, and benzylpenicillin against all tested CREnC and E. coli strains were in the range of > 1024 µg/mL, indicating resistance. However, P1 and P5 exhibited a synergistic effect against test CREnC and E. coli strains when used in combination with ceftazidime and cefotaxime, with fraction inhibitory concentration indices of < 0.062 and 0.28, respectively. A kill curve demonstrated that the combination treatments had synergistic activity and inhibited β-lactamase. Conclusion: The synergistic activity of P1 and P5 in combination with ceftazidime is achieved in multiple ways, including increased cytoplasmic and outer membrane permeability, β-lactamase inhibition, and peptidoglycan damage. Therefore, the combination therapy of Siamese crocodile plasma and ceftazidime may be a novel therapeutic approach for treating recalcitrant E. cloacae infection.

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Section: Separation Of Protein From Crocodile Plasmamentioning

confidence: 99%

Siamese crocodile plasma synergizes with ceftazidime against ceftazidime-resistant <i>Enterobacter cloacae</i>

Rojtinnakorn¹,

Temsiripong²,

Pimchan³

et al. 2018

Trop. J. Pharm Res

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Plasma vitellogenin in Morelet's crocodiles from contaminated habitats in northern Belize

Rainwater

Selcer

Nespoli

et al. 2008

Environmental Pollution

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Assessment of Vitellogenin Synthesis in Salvator merianae as a Biomarker of Exposure to Xenoestrogens in Terrestrial Ecosystems

Sánchez-Loria,

García-Valdez,

Hernández

2024

AZL

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The expansion of agriculture and livestock in Argentina has significantly increased the use of pesticides, adversely impacting animal and human health. These chemical compounds, acting as xenoestrogens, can disrupt the reproductive physiology of wildlife. In this context, vitellogenin, a liver protein typically elevated in oviparous females during reproduction, can also be induced in males and immature females by exposure to xenoestrogens. The aim of this work was to study the production of vitellogenin in response to exogenous estrogens in male Salvator merianae lizards, a species widely distributed in Argentina. Eighteen adult tegus from the Universidad Nacional de Tucumán were used, distributed into three groups: males treated with 17 ?-estradiol, females in the vitellogenesis phase (positive control), and untreated males (negative control). Treated males received doses of 17 ?-estradiol every three days for two weeks, and blood samples were taken before and during treatment for analysis by SDS-PAGE, spectrophotometry, and immunohistochemistry. Results revealed a significant increase in triglyceride levels and the synthesis of a high molecular weight lipoprotein in treated males, a pattern similar to that observed in females during vitellogenesis and absent in negative controls. This pioneering study demonstrates the induction of hepatic vitellogenin in Salvator merianae males treated with estradiol, highlighting the potential utility of this species as a biomarker of environmental contamination by xenoestrogens.

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Development of an enzyme-linked immunosorbent assay for vitellogenin of Morelet's crocodile (Crocodylus moreletii)

Cited by 4 publications

References 56 publications

Siamese crocodile plasma synergizes with ceftazidime against ceftazidime-resistant <i>Enterobacter cloacae</i>

Siamese crocodile plasma synergizes with ceftazidime against ceftazidime-resistant <i>Enterobacter cloacae</i>

Plasma vitellogenin in Morelet's crocodiles from contaminated habitats in northern Belize

Assessment of Vitellogenin Synthesis in Salvator merianae as a Biomarker of Exposure to Xenoestrogens in Terrestrial Ecosystems

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