Development of an enzyme-linked immunosorbent assay for equine neutrophil elastase measurement in blood: Preliminary application to colic cases

Effect of intensive exercise on plasmatic neutrophil elastase level in eventing and endurance horses

“…1999). Despite its wellknown proteinolyticproperties, the role of NE is probably underestimated (De la Rebière de Pouyade et al . 2009b).…”

“…2009a). An ELISA assay specific for equine NE was developed (De la Rebière de Pouyade et al . 2009b).…”

EquiNox2: A new method to measure NADPH oxidase activity and to study effect of inhibitors and their interactions with the enzyme

“…25) was similar to that previously determined for the CFS of equine phagocytic NOX [13], but with a 2-fold higher protein concentrations. The buffer volume for an optimal fixation was 100 µl, a volume usually used for enzymatic and immunological tests [9,28]. The optimal incubation time and temperature for the protein binding were 2h and 4°C, which are easy working conditions.…”

“…Among them, curcumin is a natural compound that has been widely studied for its antiinflammatory and antioxidant capacities both on human [38][39][40] and horse [9,13,24,41]. Our team already demonstrated that the molecule, and its water-soluble form NDS27 (curcumin lysinate complexed with 2-hydroxypropyl-beta-cyclodextrin (HPβCD)) [18], can interfere with the phagocytic NOX assembly in CFS.…”

“…In some pathological conditions of acute or chronic inflammation, the uncontrolled activation of PMNs is responsible for ROS releases in the extracellular medium causing deleterious effects on neighbouring cells. Unlike other species, horses are particularly sensitive to inflammation [8] and suffer from inflammatory pathologies such as laminitis, recurrent airway obstruction or intestinal strangulation that are often associated with an endotoxic shock [9,10]. Several research groups highlighted the PMNs implication in these pathologies, with a release of inflammatory mediators, myeloperoxidase, proteases, ROS,… It appears that the modulation of enzymes involved in neutrophil stimulation and ROS production such as Nox2 becomes therapeutic targets to treat pathologies with excessive inflammation [4,5,11].…”