Development of an enzyme-linked immunosorbent assay for diniconazole in agricultural samples

Determination of Diniconazole in Agricultural Samples by Sol-Gel Immunoaffinity Extraction Procedure Coupled with HPLC and ELISA

“…An indirect competitive ELISA for diniconazole was developed and was demon- strated to successfully detect diniconazole in water, pear and tomato samples. The ELISA was shown to have a higher sensitivity and specific than those of already reported polyclonal antibody-based ELISA [13] , and the sensitivity (IC 50 value) was 0.0500 mg L −1 and the cross-reactivity was below 1.82%. A good correlation between the ELISA and GC results was obtained for the spiked samples.…”

“…There was no obvious cross-reactivity (CR) with most of the triazoles tested; only uniconazole and hexaconazole showed any cross-reactivity ( Figure 3 ), the highest cross-reactivity was found to be uniconazole (1.82%), due to the same structure except for the number of chloro substituents on the benzene between uniconazole and diniconazole [13]. The values of cross-reactivity for both dinicona- zole and hexaconazole were 0.920% which may be due to a similar structure for the 2, 4-dichlorophenyl group.…”

“…Diniconazole hapten was synthesized from diniconazole and succinic anhydride in acylation reaction as reported previously [13] .…”

“…Enzyme-linked immunosorbent assay (ELISA) is the basis of fast, sensitive, cost-effective, and selective method for the detection of pesticide residues. An ELISA based on a polyclonal antibody against diniconazole was first developed by Jiang et al, which used large volumes of organic solvents and a multi-step extraction procedure from complex samples purification [13] .…”

Development of monoclonal antibody-based competitive immunoassays for the detection of picoxystrobin in cereal and oilseed flours

“…Whilst ELISA is a well-established methodology for the detection and analysis of certain chemical contaminants in cereals such as mycotoxins (Goryacheva, Rusanova, Burmistrova, & De Saeger, 2009;Skerritt, 1998), few studies have been published for the determination of fungicide residues by enzyme immunoassay in those complex food matrices (Danks, Chaudhry, Parker, Barker, & Banks, 2001;Jiang, Shi, Wu, & Wang, 2011). In previous publications, we described the syntheses of three PC derivatives functionalized with the same linker at rationally-selected sites of the target molecule and the application of selected immunoassays to the analysis of PC residues in beer (EsteveTurrillas et al, 2010).…”