Development of an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Ringworm Infection in Cattle

Bagut, E. T.; Cambier, Ludivine; Heinen, M.; Cozma, Vasile; Monod, Michel; Mignon, Bernard

doi:10.1128/cvi.00243-13

Cited by 13 publications

(3 citation statements)

References 36 publications

(40 reference statements)

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“…Humeral immune response was assessed by measuring anti T. verrucosum antibodies using direct method of ELISA according to (Sparkes et al, 1995;Bagut et al, 2013).…”

Section: Elisa Testsmentioning

confidence: 99%

EVALUATION OF THE IMMUNE RESPONSE TO <i>T. VERRUCOSUM</i> VACCINES

Zahran¹,

Abdeen²

2013

American Journal of Immunology

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Dermatophytosis is considered as one of the most important fungal skin disease affecting both humans and animals otherwise its importance as zoonotic disease. Treatment of ringworm in cattle is expensive and time consuming especially on herd level. Vaccination can be a giant step forward to prevent the occurrence of mycozoonosis between the affected animals with ringworm and susceptible man. Fifty skins scraping samples were collected from animals (cattle, sheep and calves) suffering from skin lesions suspected to be ringworm. Then these collected samples were subjected to mycological examination and identification. T. verrucosum is the main causative agent of collected skin scrapings. Two types of vaccines were prepared from this strain (Culture filtrate and formalin inactivated vaccines). Rabbit model was used for challenge testing of the vaccines. The rabbits were separated into four groups as follow: Each group contained 3 rabbits each one was injected subcutaneously with 1ml of culture filtrate with adjuvant twice for seven days intervals. Group No. 2 rabbits each one was injected intramuscularly with 1 mL formalin-inactivated with adjuvant. The 3rd group is control positive and 4th one is control negative. Vaccination programme is done via injection twice with one week interval and the challenge by scarification of the skin with 0.2 mL of 5×10 7 fungal elements/mL. For 3 days. Humeral immune response was assessed by measuring anti-T. verrucosum antibodies using ELISA technique (direct method). By using statistical analysis of ANOVA, the mean optical density of anti-T. verrucosum specific IgG level in rabbits artificially immunized with culture filtrate with adjuvant vaccine significantly increase from (1.97) post first vaccination to reach (2.43) post second vaccination and still high after challenge with the virulent T. verrucosum strain (2.32) when compared with control positive (1.57). The results revealed that both type of vaccines induce good humeral immune response in vaccinated animals. Culture filtrate vaccine used as a treatment in infected farm animals by two doses within 2 weeks and noted that healing occurred.

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“…Humeral immune response was assessed by measuring anti T. verrucosum antibodies using direct method of ELISA according to (Sparkes et al, 1995;Bagut et al, 2013).…”

Section: Elisa Testsmentioning

confidence: 99%

EVALUATION OF THE IMMUNE RESPONSE TO <i>T. VERRUCOSUM</i> VACCINES

Zahran¹,

Abdeen²

2013

American Journal of Immunology

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“…The mean of the optical density of all negative samples ( n = 46) plus three-fold standard deviation of all negative controls (Mean + 3 × SD) was designated as the cut-off point for distinguishing between positive and negative results, as described previously [30].…”

Section: Resultsmentioning

confidence: 99%

Diagnostic Performance of Dengue Virus Envelope Domain III in Acute Dengue Infection

Nguyen

Duong

Azam

et al. 2019

IJMS

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Dengue, one of the most prevalent illnesses caused by dengue viruses that are members of the genus Flavivirus, is a significant global health problem. However, similar clinical symptoms and high antigenic homologies with other Flaviviruses in the endemic area pose difficulties for differential diagnosis of dengue from other arbovirus infections. Here, we investigated four types of recombinant envelope protein domain III (DV-rED III) derived from four dengue virus (DENV) serotypes for diagnostic potential in detecting IgM in acute phase (mainly 2–3 days after onset of fever). Each independent DV-1, -3, and -4-rED III-ELISA showed less than 60% sensitivity, but the combined results of DV-1, -3, and -4-rED III-ELISA led to sensitivity of 81.82% (18/22) (95% CI, 59.72 to 94.81) and 100% specificity (46/46) (95% CI, 92.29 to 100.00) as each antigen compensated the other antigen-derived negative result. In conclusion, the independent combination of data derived from each recombinant antigen (DV1-, DV3-, and DV4-rED III) showed comparable efficacy for the detection of IgM in patients with acute-phase dengue infection.

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“…Detection of specific antibodies against DppV gave 89.6% sensitivity, 92.7% specificity, a 96.8% positive predictive value, and a 78.4% negative predictive value. The recombinant TruLap2-based ELISA displayed 88.1% sensitivity, 90.9% specificity, a 95.9% positive predictive value, and a 75.7% negative predictive value [41].…”

Section: Serodiagnosismentioning

confidence: 95%

An Over View of Bovine Dermatophytosis

Abdalla

2019

AJRAVS

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Dermatophytosis is a superficial fungal infection of hair and keratinized layers of the epidermis and is caused by keratinophilic and keratinolytic genera such as Microsporum, Trichophyton and Epidermophyton. It is an endemic infection in many countries throughout the world affecting companion animals (dogs, cats), domestic animals (calves), and laboratory animals (rabbits) as well as humans. T. verrucosum considered the main cause of ringworm in cattle. The typical lesion is a heavy, grey-white crust, circular and raised above the skin most frequently found on the head and neck. The disease can be diagnosed by direct examination, fungal culture, skin biopsy sero and molecular diagnosis methods. This review will forecast more light of the different aspects of this disease.

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Development of an Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Ringworm Infection in Cattle

Cited by 13 publications

References 36 publications

EVALUATION OF THE IMMUNE RESPONSE TO <i>T. VERRUCOSUM</i> VACCINES

EVALUATION OF THE IMMUNE RESPONSE TO <i>T. VERRUCOSUM</i> VACCINES

Diagnostic Performance of Dengue Virus Envelope Domain III in Acute Dengue Infection

An Over View of Bovine Dermatophytosis

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