2013
DOI: 10.1128/cvi.00245-13
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Development of an Enzyme-Linked Immunosorbent Assay Using a Recombinant LigA Fragment Comprising Repeat Domains 4 to 7.5 as an Antigen for Diagnosis of Equine Leptospirosis

Abstract: Leptospira immunoglobulin (Ig)-like (Lig) proteins are a novel family of surface-associated proteins in which the N-terminal 630 amino acids are conserved. In this study, we truncated the LigA conserved region into 7 fragments comprising the 1st to 3rd (LigACon1-3), 4th to 7.5th (LigACon4-7.5), 4th (LigACon4), 4.5th to 5.5th (LigACon4.5-5.5), 5.5th to 6.5th (LigACon5.5-6.5), 4th to 5th (LigACon4-5), and 6th to 7.5th (LigACon6-7.5) repeat domains. All 7 recombinant Lig proteins were screened using a slot-shaped… Show more

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Cited by 13 publications
(29 citation statements)
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“…The DNA insert of each clone was verified by DNA sequencing, and the recombinant plasmid was then transformed into E. coli BL21(DE3) (Stratagene, Santa Clara, CA) for expression. Protein expression and purification were performed as previously described (11). The concentration of purified protein was then determined using the Bradford method, and the protein was finally used for ELISA (11).…”
Section: Methodsmentioning
confidence: 99%
See 4 more Smart Citations
“…The DNA insert of each clone was verified by DNA sequencing, and the recombinant plasmid was then transformed into E. coli BL21(DE3) (Stratagene, Santa Clara, CA) for expression. Protein expression and purification were performed as previously described (11). The concentration of purified protein was then determined using the Bradford method, and the protein was finally used for ELISA (11).…”
Section: Methodsmentioning
confidence: 99%
“…Protein expression and purification were performed as previously described (11). The concentration of purified protein was then determined using the Bradford method, and the protein was finally used for ELISA (11).…”
Section: Methodsmentioning
confidence: 99%
See 3 more Smart Citations