Development of an enzyme immunoassay for the serodiagnostic of tuberculosis and mycobacterioses

Maes, Roland; Jp, Homasson; Kubín, M; Bayer, Michaela

doi:10.1007/bf00197451

Cited by 23 publications

(24 citation statements)

References 14 publications

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“…In particu lar. the evaluation of IgG directed against the antigen 60 (A60) [12][13][14] -the main thermostable component of old tuberculin and purified protein derivative -provided high diagnostic relevance [15][16][17][18][19], On the other hand, dis cordant results were reported on the diagnostic accuracy of evaluation of the IgM-mcdiated humoral immune response [20,21], In this work anti-A60 IgA levels in patients with PTb were measured by ELISA and compared to the anti-A60 IgG titers.…”

Section: Introductionmentioning

confidence: 99%

IgA Immune Response against the Mycobacterial Antigen A60 in Patients with Active Pulmonary Tuberculosis

Alifano

Miceli²,

Mormile³

et al. 1996

Respiration

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Searching for IgG and IgM against the mycobacterial antigen A60 has been recognized as a potential diagnostic tool for pulmonary tuberculosis. The role of detection of anti-A60 IgA in improving diagnostic accuracy of serology is not well known. In this study we measured with ELISA serum levels of both anti-A60 IgG and IgA in 216 subjects. 88 healthy volunteers (44 PPD- and 44 PPD+), 44 patients suffering from nontuberculous lung disease and 15 subjects with healed pulmonary tuberculosis constituted the control population; 69 patients with active pulmonary tuberculosis (35 cavitary forms, 26 productive forms and 8 miliary forms) were examined. The sensitivity of IgG test was 73.9% in pulmonary tuberculosis (77.1% in cavitary forms, 65.4% in productive forms, 87.5% in milary forms); the specificity of the test was 95.9%. For the IgA test we observed a sensitivity of 72.5% (74.3 in cavitary forms, 69.2% in productive forms, 75.0 in miliary forms) and a specificity of 93.9%. Combination of the two tests increased the sensitivity to 84.0% (+10.1 % compared to IgG test, +11.5% compared to IgA test); the specificity decreased to 92.5% (-3.4% vs. IgG test; -1.4 vs. IgA test). In conclusion, the combined use of evaluation of anti-A60 IgG and IgA increases the accuracy of serological diagnosis of pulmonary tuberculosis.

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Section: Introductionmentioning

confidence: 99%

IgA Immune Response against the Mycobacterial Antigen A60 in Patients with Active Pulmonary Tuberculosis

Alifano

Miceli²,

Mormile³

et al. 1996

Respiration

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“…The interferon test is a costly substitute to a much cheaper method of assessment of latent infections, by monitoring the humoral immunity. The Anda TB serological test is available since 1989 [58] was shown useful for the detection of latent infections in exposed groups in 1989 [59] and 1990 [60], among some cancer patients in 1995 [61]. Serological measurements also give valuable prognostic and diagnostic clues in BCG vaccinated infants [22].…”

Section: Prognosis and Diagnosismentioning

confidence: 99%

Can the Triumphant March of TB be Stopped? A Critical Appraisal of the Currently Applied Policy of Eradication

Maes¹

2016

Clin Microbiol

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The fight against TB is organized by International (WHO, International Union of Tuberculosis and Lung Diseases (UTLD) and National organizations (Ministries of Health). Their strategy is based on a "herd medicine" approach. The vaccine and the massive administration of four drugs (isoniazid, rifampicin, ethambutol and pyrazinamide-with streptomycin eventually added for the resistant cases) worldwide during decennia were expected to solve the TB problem. They did not because the means applied to fight the disease were not appropriate. The BCG vaccine is the only vaccine in use, yet it sometimes promotes TB multiplication instead of eradication; only four drugs were imposed by Standard Operating Procedures during decennia, of which some are immunodepressive and may, under some conditions, add to the immunodepressive activity of the pathogen and favour relapse; the diagnosis limited to the detection of the pathogen ignores the immune condition of the patients under treatment.The prognosis, diagnosis and treatment of tuberculosis can be improved. The BCG vaccine is iatrogenic and needs replacement; diagnostic tests based on the detection of the pathogen have their use but need to be completed with the detection of asymptomatic cases and the monitoring of the immune status of the treated patients with a sero-diagnostic test that detects unapparent infections and immune-depressed cases; the drugs used to fight TB must be re-evaluated and complemented by the application of immune-stimulating products to those refractory patients who show a need therefore. The continuing use of an iatrogenic vaccine and of immunodepressive drugs defeats the purpose.

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“…The hummoral immune response following BCG vaccination indicates that IgM anti PPD increase progressively until the third month, followed by an increase of IgG. The antibody dynamics in TB infection could be similar (Maes et al, 1989), but it also depends on the clinical form and treatment starting. IgA serum antibodies appear immediately after IgM and precede IgG, but the CSF dynamics could vary.…”

Section: The Dynamic Synthesis Of Antimycobacterial Antibodiesmentioning

confidence: 99%

Immunologic Diagnosis of Neurotuberculosis

Alexandra¹,

Dorina²,

Gabriela³

et al. 2012

Understanding Tuberculosis - Global Experiences and Innovative Approaches to the Diagnosis

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Development of an enzyme immunoassay for the serodiagnostic of tuberculosis and mycobacterioses

Cited by 23 publications

References 14 publications

IgA Immune Response against the Mycobacterial Antigen A60 in Patients with Active Pulmonary Tuberculosis

IgA Immune Response against the Mycobacterial Antigen A60 in Patients with Active Pulmonary Tuberculosis

Can the Triumphant March of TB be Stopped? A Critical Appraisal of the Currently Applied Policy of Eradication

Immunologic Diagnosis of Neurotuberculosis

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