Development of an engineered Bacillus subtilis strain for antibiotic‐free sucrose isomerase production
Mingyu Li,
Xiaopeng Ren,
Ming Xu
et al.
Abstract:Sucrose isomerase (SIase) catalyzes the hydrolysis and isomerization of sucrose into isomaltulose, a functional sugar extensively used in the food industry. However, the lack of safe and efficient heterologous expression systems for SIase has constrained its production and application. In this study, an engineered Bacillus subtilis strain for antibiotic‐free SIase production was developed via a food‐grade expression system. First, the B. subtilis strain TEA was modified through the CRISPR/Cas9 system, resultin… Show more
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