Development of an EnFET for the detection of organophosphorous and carbamate insecticides

Flores, Francisco; Artigas, José María Gatell; Marty, Jean‐Louis; Valdés, Francisco

doi:10.1007/s00216-003-1925-y

Cited by 17 publications

(9 citation statements)

References 22 publications

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“…This is a critical step since total regeneration is not usually achieved and dependence on membrane thickness and enzyme concentration is present. Other types of ENFETs using immobilization techniques in activated Nylon nets [78] and combining polymers like PVC and PVA have also been described [79,80]. Until now, none of these ISFET based sensors developed for pesticides monitoring have been applied to real samples due to the difficulties outlined.…”

Section: Isfets Applications In the Environmental Fieldmentioning

confidence: 99%

ISFET Based Microsensors for Environmental Monitoring

Jimenez-Jorquera

Orozco

Baldi

2009

Sensors

151

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The use of microsensors for in-field monitoring of environmental parameters is gaining interest due to their advantages over conventional sensors. Among them microsensors based on semiconductor technology offer additional advantages such as small size, robustness, low output impedance and rapid response. Besides, the technology used allows integration of circuitry and multiple sensors in the same substrate and accordingly they can be implemented in compact probes for particular applications e.g., in situ monitoring and/or on-line measurements. In the field of microsensors for environmental applications, Ion Selective Field Effect Transistors (ISFETs) have a special interest. They are particularly helpful for measuring pH and other ions in small volumes and they can be integrated in compact flow cells for continuous measurements. In this paper the technologies used to fabricate ISFETs and a review of the role of ISFETs in the environmental field are presented.

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Section: Isfets Applications In the Environmental Fieldmentioning

confidence: 99%

ISFET Based Microsensors for Environmental Monitoring

Jimenez-Jorquera

Orozco

Baldi

2009

Sensors

151

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“…The variation in the electric potential reflects changes in the concentration of H + ions distributed in the solution. 18 The measurements were repeated for three different PAH/SiO 2 -Np-AChE modified electrodes so that a calibration curve (Fig. 3) could be obtained.…”

Section: Resultsmentioning

confidence: 99%

Self-Assembly of SiO<SUB>2</SUB> Nanoparticles for the Potentiometric Detection of Neurotransmitter Acetylcholine and Its Inhibitor

Arruda¹,

Guimarães²,

Ramos³

et al. 2014

j nanosci nanotechnol

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The detection and quantification of neurotransmitter acetylcholine (ACh) are relevant because modifications in the ACh levels constitute a threat to human health. The biological regulator of this neurotransmitter is acetylcholinesterase (AChE), an enzyme that catalyzes the hydrolysis of ACh to choline and acetic acid. However, its activity is inhibited in the presence of organophosphate and carbamate pesticides, compromising the degradation of the neurotransmitter. There has been a growing interest in faster and more sensitive detection systems that include new methods and materials for the determination of the ACh concentration. This paper proposes a potentiometric biosensor for the detection of neurotransmitter ACh and its inhibitors, specifically organophosphate pesticide methamidophos. The biosensor is based on a self-assembled platform formed by poly(allylamine) hydrochloride (PAH) and silicon dioxide nanoparticles (SiO 2 -Np) that contains the immobilized enzyme AChE. First, the responses of the biosensor were investigated for different concentrations of ACh in buffer solutions. After quantifying ACh, the inhibition of AChE in the presence of methamidophos was determined, enabling the quantification of methamidophos expressed as the percentage of enzyme inhibition. The potential advantages of this biosensor include simplicity in building the electrode, possible production on an industrial scale, limited need for qualified personnel to operate the device and low processing cost.

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“…Biosensor for OP insecticides based on the AChE inhibition test, using amperometric [77, 89-92, 114, 115], potentiometric [99,116], conductimetric [112], and fiber optic [101,103,104] transducers have been reported. As target of insecticides, the AChE from different sources were genetically modified to enhance their characteristics as specificity and turnover [34,49].…”

Section: Biosensors Sensitivity and Selectivitymentioning

confidence: 99%

Use of Esterase Activities for the Detection of Chemical Neurotoxic Agents

Manco¹,

Nucci²,

Febbraio

2009

PPL

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The quest for a quick and easy detection of the neurotoxin levels in the environment has fostered the search for systems alternative to currently employed analytical methods such as spectrophotometer, gas-liquid chromatography, thin-layer chromatography, and more recently mass spectrometry. These drawbacks lead to intense research efforts to develop biosensor devices for the determination of these compounds. In this review, we present an overview of the actual development of research in neurotoxin detection by using enzymatic biosensors based on esterase activity, in particular cholinesterases, and carboxylesterases. Detection by enzymatic activity could be carried out measuring the hydrolysis products or the residual enzymatic activity after inhibition, using a transducer system that makes possible the correlation between the determined activity and the analyte concentration. Several transducer systems were adopted for the neurotoxins identification using esterases, including electrochemical, optical, conductimetric and piezoelectric procedures. The differences in the used transducer determine the final sensitivity and specificity of the biosensor. Moreover, a brief description of immobilization procedure, that is an important step in the biosensor development and could affect the final characteristic of biosensor (sensibility, stability, response time and reproducibility), was accomplished. Final considerations on advantages and problems, related to actual development of these technologies, and its prospective were discussed.

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Development of an EnFET for the detection of organophosphorous and carbamate insecticides

Cited by 17 publications

References 22 publications

ISFET Based Microsensors for Environmental Monitoring

ISFET Based Microsensors for Environmental Monitoring

Self-Assembly of SiO<SUB>2</SUB> Nanoparticles for the Potentiometric Detection of Neurotransmitter Acetylcholine and Its Inhibitor

Use of Esterase Activities for the Detection of Chemical Neurotoxic Agents

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