Development of an ELISA using polyclonal antibodies specific for 2-methylisoborneol

Chung, Si Yin; Johnsen, Peter B.; Klesius, Phillip H.

doi:10.1021/jf00092a015

Cited by 19 publications

(16 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the use of fish management practices that foster the production of leaner more uniform populations may reduce the con- centrations of off-flavor metabolites in fish and increase the accuracy of flavor quality control decisions that are based upon evaluations of representative samples of fish. Efforts have been made to improve the detection and quantification of MIB, geosmin and other problematic metabolites in fish and water samples, and investigate conditions affecting noxious phytoplankton bloom dynamics in aquaculture systems (Johnsen and Kuan 1987;Chung et al 1990;Chung et al 1991;Millie et al 1995;Conte et al 1996aConte et al , 1996bGrimm et al 1996aGrimm et al , 1996bMiyamoto 1997). In addition, means to avoidhnhibit the biosynthesis of off-flavor metabolites and their occurrence in fish are being investigated.…”

Section: Potential Responses To Mixed-jlavor-populationsmentioning

confidence: 99%

Variation in Channel Catfish Ictalurus punctatus Flavor Quality and its Quality Control Implications

Dionigi

Bett

Johnsen

et al. 1998

J World Aquaculture Soc

Self Cite

View full text Add to dashboard Cite

Producers may capture two to three channel catfish Ictalurus punctatus L. for flavor analysis from ponds scheduled for harvest. If off-flavors are not present in several consecutive fish samples, the population may be considered acceptable for harvest. However, instrumental analysis of the muddy/earthy off-flavor metabolites 2-methylisoborneol (l-R-exo-1,2,7,7-tetramethyl-bicyclo-[2,2,l]-heptan-2-ol) (MIB) and geosmin (la, lO~-dimethyl-9a-decalol) concentrations in approximately 80 catfish from each of three commercial ponds found both acceptable (on-flavor) and unacceptable (off-flavor) individuals within a single pond (i.e., mixed-flavor populations). Ascertaining the frequency of mixed-flavor populations by instrumentally determining the off-flavor metabolite concentration in muscle tissues from a large number of fish sampled from many ponds at several locations over time is not currently feasible. However, analysis of 12,725 commercial processor flavor assessments collected in 1994 and 1995 indicated 120 instances of individual ponds yielding off-flavor fish followed by on-flavor fish in one day or less. Reports indicate that fish require approximately four days to depurate off-flavors fully, suggesting that a proportion of these rapidly changing flavor assessments may reflect the stochastic selection of fish from mixed-flavor populations rather than a complete and rapid conversion of the flavor quality of entire populations. Factors that contribute to the Occurrence of mixed-flavor-populations have not been identified fully. However, increased proportion of fat in catfish fillet tissues has been reported to be correlated with a greater retention of MIB by fish. In this investigation, fish fat contents ranged from 4.45% to 30.45%, and were positively correlated (P < 0.OOOl) with MIB concentrations.Additionally, the spatial distributions of algal populations within certain commercial catfish ponds were not uniform, and the sensory analysis of the flavor intensity of MIB and geosmin in catfish was more variable than the assessment of the intensity of chickeny and nutty flavors. Probability analysis indicated that in certain mixed-flavor populations there was a 10 to 25% probability of a shipment of fish being rejected due to the random collection of only on-flavor fish prior to harvest followed by the post harvest capture of a t least one off-flavor fish. In addition, a chance of no off-flavor fish being sampled from a population containing a proportion of unacceptable fish was indicated. At current sample sizes, replacing sensory analysis with instrumental analysis would not completely avoid problems associated with sampling mixed-flavor-populations. Until effective means to reducelavoid off-flavor metabolite accumulations in fish are widely available, careful attention to proper sensory evaluation protocols, an enhanced attention to pond conditions that affect flavor quality, the production of leaner more uniform populations of fish, and optimizations of sampling strategies offer the most practical near-term ...

show abstract

Section: Potential Responses To Mixed-jlavor-populationsmentioning

confidence: 99%

Variation in Channel Catfish Ictalurus punctatus Flavor Quality and its Quality Control Implications

Dionigi

Bett

Johnsen

et al. 1998

J World Aquaculture Soc

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sections were incubated in blocking buffer (10% normal goat antiserum : double-strength PBS) for 1 h and washed 2 x in double-strength PBS for 15 min each. Primary antibody to MIB was raised in goats (Chung et al 1990) and either was used as a crude antiserum (diluted 1 : 100 in blocking buffer) or was purified using a protein-G-activated purification cartridge (FMC, Inc., Rockland, ME) (diluted 1:125 in blocking buffer). Sections were incubated in primary antiserum for 1 h at room temperature and washed 4 x in double-strength PBS.…”

mentioning

confidence: 99%

INTRACELLULAR LOCALIZATION OF THE TASTE/ODOR METABOLITE 2‐METHYLISOBORNEOL IN OSCILLATORIA LIMOSA (CYANOPHYTA)¹

Bafford

Seagull

Chung

et al. 1993

Journal of Phycology

View full text Add to dashboard Cite

The monoterpene derivative, 2‐methylisoborneol (MIB), is produced by many blue‐green algae and often is responsible for the “musty” taste/odor in aquaculturally raised finfish and potable water supplies. Although previous researchers have suggested that taste/odor metabolites are partitioned among cell constituents and that coregulation with pigment biosynthesis occurs, no structural evidence for these hypotheses exists. MIB was localized in cells of Oscillatoria limosa (Roth) Agardh at the ultrastructural level using standard gold‐labeled antibody techniques. There was no apparent relationship between age of the cells and MIB synthesis; cells that were and were not undergoing active division had similar amounts of MIB label. There was no consistent partitioning of MIB label within cells. However, occasionally, specific label was observed along photosynthetic lamellae, suggesting a potential linkage of MIB synthesis and/or binding to the pigment systems.

show abstract

“…Chung et al (28) and Chung (29) prepared polyclonal antibodies to a camphor-bovine serum albumin (BSA) conjugate. The resulting immunoassay gave a detection limit of only 1 mg L -1 and had 100% cross-reactivity to both camphor and the MIB dehydration product 2-methyl-2-borene.…”

Section: -Methylisoborneol (Mibmentioning

confidence: 99%

High-Affinity Monoclonal Antibodies for Detection of the Microbial Metabolite, 2-Methylisoborneol

Plhak

Park

2003

J. Agric. Food Chem.

View full text Add to dashboard Cite

The production of 2-methylisoborneol (MIB) by certain fungi and algae can contribute musty off-flavors to foods and water supplies if uncontrolled. The goal of this research was to develop a nonsensory simple method for the detection of MIB. Anti-MIB monoclonal antibodies were produced by immunizing mice with borneol-conjugated protein and selecting positive clones with an MIB-protein conjugate. An indirect competitive immunoassay developed using this antibody had a detection limit of 0.6 microg L(-)(1) and an I(50) value of 5 microg L(-)(1). Detection was relatively specific for MIB and showed 20% cross-reactivity with borneol or isoborneol and 4-5% cross-reactivity with camphor. No cross-reactivity to geosmin was observed.

show abstract

Development of an ELISA using polyclonal antibodies specific for 2-methylisoborneol

Cited by 19 publications

References 20 publications

Variation in Channel Catfish Ictalurus punctatus Flavor Quality and its Quality Control Implications

Variation in Channel Catfish Ictalurus punctatus Flavor Quality and its Quality Control Implications

INTRACELLULAR LOCALIZATION OF THE TASTE/ODOR METABOLITE 2‐METHYLISOBORNEOL IN OSCILLATORIA LIMOSA (CYANOPHYTA)¹

High-Affinity Monoclonal Antibodies for Detection of the Microbial Metabolite, 2-Methylisoborneol

Contact Info

Product

Resources

About

Development of an ELISA using polyclonal antibodies specific for 2-methylisoborneol

Cited by 19 publications

References 20 publications

Variation in Channel Catfish Ictalurus punctatus Flavor Quality and its Quality Control Implications

Variation in Channel Catfish Ictalurus punctatus Flavor Quality and its Quality Control Implications

INTRACELLULAR LOCALIZATION OF THE TASTE/ODOR METABOLITE 2‐METHYLISOBORNEOL IN OSCILLATORIA LIMOSA (CYANOPHYTA)1

High-Affinity Monoclonal Antibodies for Detection of the Microbial Metabolite, 2-Methylisoborneol

Contact Info

Product

Resources

About

INTRACELLULAR LOCALIZATION OF THE TASTE/ODOR METABOLITE 2‐METHYLISOBORNEOL IN OSCILLATORIA LIMOSA (CYANOPHYTA)¹