Development of an ELISA to detect clenbuterol in swine products using a new approach for hapten design

Bui, Quoc Anh; Vu, Thi Huynh Han; Ngo, Vo Ke Thanh; Kennedy, Ivan R.; Lee, Nanju Alice; Allan, Robin D.

doi:10.1007/s00216-016-9750-2

Cited by 24 publications

(5 citation statements)

References 10 publications

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“…Especially, the characteristic structural activity of the hapten may have huge effects on it. Although the significance of spacer arm properties (length, flexibility, and rigidity) has been discussed in many studies, − there remains a lack of in-depth exploration of a specific relationship between the structural activity of hapten and their immunoinducing ability. Hence, we attempted to infer hapten structure that is more favorable for inducing antibody production based on their structural information (e.g., three-dimensional conformation, size, charge distribution, antibody–antigen recognition).…”

Section: Resultsmentioning

confidence: 99%

Rapid Screening of Emergent Febuxostat Adulteration in Functional Foods Based on a Simulation-Inspired High-Quality Antibody

Guan,

Liu,

et al. 2024

J. Agric. Food Chem.

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Due to the potential health risks of adulterated febuxostat in uric-acid-lowering foods, it is urgent to develop rapid detection methods. However, there are no fast analytical techniques for febuxostat yet. Herein, an efficient hapten simulation strategy was proposed to successfully produce a highly sensitive and selective monoclonal antibody toward febuxostat. Based on such a robust recognition element, easy colorimetric and ultrasensitive fluorescent lateral flow immunochromatographic immunoassays were first established, which can detect febuxostat as low as 60 μg/kg by the naked eye or 1.01 μg/kg by a commercial test strip reader with acceptable stability. Furthermore, in the recovery test and blind sample analysis, consistent results between our methods and the authorized liquid chromatography-tandem mass spectrometry method suggested the high accuracy and practicality of this work. The present work not only proposes a rational hapten design idea but also provides favorable tools for the rapid screening of febuxostat in functional foods.

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Section: Resultsmentioning

confidence: 99%

Rapid Screening of Emergent Febuxostat Adulteration in Functional Foods Based on a Simulation-Inspired High-Quality Antibody

Guan,

Liu,

et al. 2024

J. Agric. Food Chem.

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“…Clenbuterol [35,36], salbutamol [37] and phenylethanolamine A [38] in various samples (animal urine, blood, hair and tissues) have been measured using direct competitive ELISA. The mAb or pAb against β 2 -agonist is coated onto the microtiter plate, the β-agonist in sample and β 2 -agonist labeled with HRP or ALP competes for binding with the mobilized antibody.…”

Section: Direct Competitive Elisamentioning

confidence: 99%

Current Advances in Immunoassays for the Detection of β2-Agonists

Ouyang

2022

Foods

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β2-agonists are a group of synthetic phenylethanolamine compounds which are traditionally used for treating bronchospasm. These compounds can also increase skeletal muscle mass and decrease body fat. The illegal use of β2-agonists in food-producing animals results in residue of β2-agonists in edible tissues and causes adverse health effects in humans. Thus, the detection of β2-agonists at trace level in complex sample matrices is of great importance for monitoring the abuse of β2-agonists. Many methods have been developed to detect β2-agonists. Among them, a variety of antigen–antibody interaction-based techniques have been established to detect β2-agonists in various samples, including animal feed, urine, serum, milk, tissues and hair. In this review, we summarized current achievement in the extraction of β2-agonists from testing samples and detection of β2-agonists using immunological techniques. Future perspectives were briefly discussed.

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“…There are many ways to detect CB, including high performance liquid chromatography (HPLC) [ 8 ], capillary electrophoresis (CE) [ 9 ], enzyme-linked immunosorbent assay (ELISA) [ 10 , 11 ], gas chromatography-mass spectrometry (GC-MS) [ 12 ], and liquid chromatography with tandem mass spectrometry (LC-MS) [ 13 , 14 ]. Although these detection methods have the advantage of high accuracy, professional operators, complex extraction steps, and expensive equipment are also needed.…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Clenbuterol Residues in Pork Using Enhanced Raman Spectroscopy

et al. 2022

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Clenbuterol (CB) is a synthetic β-receptor agonist which can be used to improve carcass leanness in swine, but its residues in pork also pose health risks. In this report, surface-enhanced Raman scattering (SERS) technology was used to achieve rapid detection and identification of clenbuterol hydrochloride (CB) residues. First, the effects of several different organic solvents on the extraction efficiency were compared, and it was found that clenbuterol in pork had a better enhancement effect using ethyl acetate as an extraction agent. Then, SERS signals of clenbuterol in different solvents were compared, and it was found that clenbuterol had a better enhancement effect in an aqueous solution. Therefore, water was chosen as the solvent for clenbuterol detection. Next, enhancement effect was compared using different concentration of sodium chloride solution as the aggregating compound. Finally, pork samples with different clenbuterol content (1, 3, 5, 7, 9, and 10 µg/g) were prepared for quantitative analysis. The SERS spectra of samples were collected with 0.5 mol/L of NaCl solution as aggregating compound and gold colloid as an enhanced substrate. Multiple scattering correction (MSC) and automatic Whittaker filter (AWF) were used for preprocessing, and the fluorescence background contained in the original Raman spectra was removed. A unary linear regression model was established between SERS intensity at 1472 cm-1 and clenbuterol content in pork samples. The model had a better linear relationship with a correlation coefficient R2 of 0.99 and a root mean square error of 0.263 µg/g. This method can be used for rapid screening of pork containing clenbuterol in the market.

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Development of an ELISA to detect clenbuterol in swine products using a new approach for hapten design

Cited by 24 publications

References 10 publications

Rapid Screening of Emergent Febuxostat Adulteration in Functional Foods Based on a Simulation-Inspired High-Quality Antibody

Rapid Screening of Emergent Febuxostat Adulteration in Functional Foods Based on a Simulation-Inspired High-Quality Antibody

Current Advances in Immunoassays for the Detection of β2-Agonists

Rapid Detection of Clenbuterol Residues in Pork Using Enhanced Raman Spectroscopy

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