Development of an electrochemical DNA biosensor with a high sensitivity of fM by dendritic gold nanostructure modified electrode

Li, Feng; Han, Xiaoping; Liu, Shufeng

doi:10.1016/j.bios.2010.11.020

Cited by 120 publications

(22 citation statements)

References 39 publications

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“…However, most dendritic nanostructures are materials not a device. 26, 27 In our previous study, a dendritic nanotip concentrated as few as 10 copies in a sample volume of 2 μL due to a large surface area with a high intensity electric field. However, precise measurement with a fluorescence microscope was required for identification of DNA.…”

Section: Introductionmentioning

confidence: 98%

Electrolyte-free amperometric immunosensor using a dendritic nanotip

et al. 2013

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Electric detection using a nanocomponent may lead to platforms for rapid and simple biosensing. Sensors composed of nanotips or nanodots have been described for highly sensitive amperometry enabled by confined geometry. However, both fabrication and use of nanostructured sensors remain challenging. This paper describes a dendritic nanotip used as an amperometric biosensor for highly sensitive detection of target bacteria. A dendritic nanotip is structured by Si nanowires coated with single-walled carbon nanotubes (SWCNTs) for generation of a high electric field. For reliable measurement using the dendritic structure, Si nanowires were uniformly fabricated by ultraviolet (UV) lithography and etching. The dendritic structure effectively increased the electric current density near the terminal end of the nanotip according to numerical computation. The electrical characteristics of a dendritic nanotip with additional protein layers was studied by cyclic voltammetry and I–V measurement in deionized (DI) water. When the target bacteria dielectrophoretically captured onto a nanotip were bound with fluorescence antibodies, the electric current through DI water decreased. Measurement results were consistent with fluorescence- and electron microscopy. The sensitivity of the amperometry was 10 cfu/sample volume (103 cfu/mL), which was equivalent to the more laborious fluorescence measurement method. The simple configuration of a dendritic nanotip can potentially offer an electrolyte-free detection platform for sensitive and rapid biosensors.

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Section: Introductionmentioning

confidence: 98%

Electrolyte-free amperometric immunosensor using a dendritic nanotip

et al. 2013

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“…43 The upper layer was a cotton-wool-like nanostructure. The real surface of the NSG electrode prepared by this strategy was larger than those of direct electrochemical deposition gold strategy, 29 dealloying strategy from silver/ gold alloys, 48 three-dimensionally ordered macroporous template strategy, 39 and hydrogen bubble dynamic template synthesis strategy.…”

Section: Results and Discussion Preparation Of Nsg Electrodementioning

confidence: 97%

“…Several methods, such as electrochemical deposition, 28,29 electrochemical erosion, 30 electrochemical oxidation/chemical reduction processes, 31 chemical dealloying of alloys, [32][33][34] electrochemical alloying/dealloying, [35][36][37] and template synthesis, [38][39][40] were reported to be used to prepare nanostructure materials. However, the catalytic activities of NSG electrodes prepared by electrochemical erosion and electrochemical oxidation/chemical reduction are low.…”

Section: Introductionmentioning

confidence: 99%

Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode

Zhong¹

2012

IJN

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Abstract:In this paper, a sensitive chronocoulometric deoxyribonucleic acid (DNA) biosensor based on a nanostructure gold electrode was fabricated for detection of the femtomolar level survivin gene which was correlated with osteosarcoma by using hexaamine-ruthenium III complexes, [Ru(NH 3 , as the electrochemical indicator. The effect of different frequencies on the real surface area of the nanostructure gold electrode obtained by repetitive square-wave oxidation reduction cycle was investigated. At the optimal frequency of 8000 Hz, the real surface of the developed nanostructure gold electrode was about 42.5 times compared with that of the bare planar gold electrode. The capture probe DNA was immobilized on the nanostructure gold electrode and hybridized with target DNA. Electrochemical signals of hexaamine-ruthenium III bound to the anionic phosphate of DNA strands via electrostatic interactions were measured by chronocoulometry before and after hybridization. The increase of the charges of hexaamineruthenium III was observed upon hybridization of the probe with target DNA. Results indicate that this DNA biosensor could detect the femtomole (fM) concentration of the DNA target quantitatively in the range of 50 fM to 250 fM; the detection limit of this DNA biosensor was 5.6 fM (signal to noise = 3). This new biosensor exhibits excellent sensitivity and selectivity and has been used for an assay of polymerase chain reaction (PCR) with a satisfactory result.

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“…Membrane protein studies [50], Photoluminescence [51], ultrasensitive biosensors [52], field enhancement [53] lithium ion batteries [54], solar cell application [55], photoresponsive liquid crystals [56] Drug delivery [57], nitrite sensing Nanocube molecular binding on membrane surfaces [58], Polyvalent DNA-Silver Nanocube Conjugates [59] increased-sensitivity localized surface plasmon resonance sensors revealed [60], electrocatalysts [61] Formic Acid Oxidation [62], methanol and ethanol electrooxidation [63] Dendritic nanostructure electrochemical DNA biosensor [64] Temperature-Sensitive Shell [65], flexible dye-sensitized solar cells [66] oxygen reduction [67], replacement reaction [68] Nanochain chemical vapor deposition [69], superior photocatalytic activity [70] surface-enhanced Raman scattering [71], water treatment [72], energy storage [73],…”

Section: Nanodiskmentioning

confidence: 99%

Advances in Silver Nanotechnology: An Update on Biomedical Applications and Future Perspectives

Aziz

Akbarzadeh

2017

Drug Res (Stuttg)

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Nanotechnology is one of the most promising fields for producing new applications in nanotechnology, biotechnology, and medicine. However, only a few products have been used for nanotechnology, biotechnology, and medical purposes. Nanoparticles have been among the most usually applied nanomaterial in our health care system for hundreds of years. The most prominent nano product is nano silver which generally present at 1-100 nm in size in at least one dimension. Silver nanoparticles reveal remarkably unusual chemical, physical and biological properties and have been used for many different applications which detailed in this review. This review is focusing on the different form of silver nanoparticles and its nanotechnological, biotechnological and medical applications.

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Development of an electrochemical DNA biosensor with a high sensitivity of fM by dendritic gold nanostructure modified electrode

Cited by 120 publications

References 39 publications

Electrolyte-free amperometric immunosensor using a dendritic nanotip

Electrolyte-free amperometric immunosensor using a dendritic nanotip

Detection of femtomolar level osteosarcoma-related gene via a chronocoulometric DNA biosensor based on nanostructure gold electrode

Advances in Silver Nanotechnology: An Update on Biomedical Applications and Future Perspectives

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