Development of an Electrochemical Cholesterol Sensor System for Food Analysis

Abstract

Preparation of self-assembly electrodeWe prepared the SAM on amorphous and (1 1 1) single-crystal gold using C18H37SH and cholesterol. The thiol, C18H37SH, is hereafter abbreviated as C18SH. For the amorphous gold 2012

Electrochemical oxidation of cholesterol

“…Next, the electrode is placed into a solution containing cholesterol, which is adsorbed in the empty spots and the oxidation current of ferrocyanide is measured again. The difference in the current values is proportional to the concentration of cholesterol [ 72 – 74 ]. These sensors have been proved to be useful for analyzing food samples [ 74 ].…”

“…The difference in the current values is proportional to the concentration of cholesterol [ 72 – 74 ]. These sensors have been proved to be useful for analyzing food samples [ 74 ].…”

Placement of Nanospace on an Electrode for Biosensing

“…The fabrication of nanospace on an electrode has been achieved using film modification techniques: self-assembly, 53,55,56,90 SiO2 sol-gel, 50,61 cross-linking, 62 electrochemical polymerization, [47][48][49]51,52,54,[57][58][59][60] and others. 91 The formation of a nanospace cavity to a target molecule essentially relies on the binding and interaction between constituents and targets.…”

“…To perform this procedure, a planar gold electrode was immersed in an ethanol solution containing cholesterol as a template and stearylmercaptan as a matrix. [55][56][57] Voltammograms from each step of the preparation and sensing are shown in Fig. 2A.…”

“…Therefore, molecular imprinting can be described as a way of making an artificial "lock" for a molecular "key". [37][38][39][40][41] The construction of an MIP on an electrode combines the molecular imprinting method with electrical and electrochemical sensing techniques, resulting in effective measurement and signaling for a variety targets, [42][43][44][45][46] amino acids, [47][48][49][50] glucose, 51,52 cholesterol, [53][54][55][56][57] bile acids, 58 and others, [59][60][61][62][63] using an artificial component to mimic the biological receptor.…”