Development of an Electrochemical, Aptamer-Based Sensor for Dynamic Detection of Neuropeptide Y

Development of a Neuropeptide Y-Sensitive Implantable Microelectrode for Continuous Measurements

“…Based on the results obtained with SWV and the results published previously by us and other research reports, the aptamer 4.31 was the best for dynamic measurements.…”

“…Physical and electrochemical characterization showed a successful surface modification on the Pt microelectrodes using EDC/Sulfo-NHS ester coupling chemistry in a rough semispherical surface. By steady-state measurements and compared with previous publications by our research group and other researchers, , it was possible to determine the best aptamer for the dynamic measurements of NPY, which was the 4.31 aptamer developed by Mendonsa et al with a K D = 0.3 ± 0.2 μM, the lowest of the aptamers tested. The optimization of the IPA frequency was carried out by comparison of the differences in each of the NPY standard solution concentrations.…”

“…Seibold et al also reported different aptamer–MB constructs in which the aptamer is internally and terminally MB-labeled. Their results showed that the terminally MB-label aptamer had a better performance in measuring the presence of NPY …”

“…This is an impediment for the SWV technique to go faster than a few scans per second. IPA, on the other hand, applies a square wave with the limit potentials on both sides of the SWV peak. , By doing this, the current measured during each half of the square wave applied measures the oxidation and reduction currents, respectively. An electrical current peak that corresponds to nonfaradaic processes (ionic currents) is expected to be observed, with a decrease that continues into faradaic currents.…”

“…Recently, Seibold et al reported the first use of aptamer-modified microelectrodes to measure NPY dynamically, which has the potential to have excellent spatial and temporal resolution . The authors were able to measure NPY in serum very accurately.…”