Abstract:Compare with transient expression, stable cell lines generally have
higher productivity and better quality for protein expression. However,
selection of stable cell line is time-consuming and laborious. Here we
describe an optimized selection method to achieve high-efficient stable
cell pools with Expi293F suspension cells. This method only takes two to
three weeks to generate stable cell pools with 2- to 10-fold higher
productivity than transient gene expression (TGE). In fed-batch culture
with Yeastolate, &g… Show more
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