Development of an effective microsatellite marker system to determine the genetic structure of <i>Meriones meridianus</i> populations

Abstract

Understanding the genetic quality of the gerbil, Meriones meridianus, plays an important role in the study of medical biology. However, no effective system has been established for evaluating a population's genetic diversity to date. In the present study, we established a set of reasonable evaluative systems based on microsatellite markers of the Mongolian gerbil by using the method of cross-amplification of species. Following electrophoresis analysis, short tandem repeat (STR) scanning, and sequencing, 11 microsatellite loci were identified by matching the criteria characteristics and were used to evaluate the genetic diversity of two stocks of Meriones meridianus: Meriones meridianus jei Wang, 1964 (M. m. jei) and Meriones meridianus cryptorhinus Blanford, 1875 (M. m. cryptorhinus) from Xinjiang, China. The microsatellite loci screened were highly polymorphic and were suitable for genetic quality control of Meriones meridianus. In addition, the quality of the non-bred M. m. jei and M. m. cryptorhinus strains in our study is sufficient for them to be promising stocks in the future for the farmed animal industry.

Development of Microsatellite Marker System to Determine the Genetic Diversity of Experimental Chicken, Duck, Goose, and Pigeon Populations

“…Microsatellite can be used to distinguish heterozygous from homozygous because of their codominant inheritance feature [ 29 ]. In previous studies, microsatellites have been used as biomarkers for monitoring rodent genetic traits [ 30 , 31 ]. With the deep understanding of microsatellites, it plays a more important role in genetic monitoring for being simple, clear, and stable in operation.…”

An investigation of genetic diversity in three Dezhou donkey original breeding farms

“…DNA was extracted from blood using the M5 FlexGen Blood DNA kit (TIANGEN, Beijing, China). The DNA purity (OD260/OD280) was measured by spectrophotometer (B500, Metash, Shanghai, China), and the quantity of genomic DNA extracted was measured using 1% agarose gel 12 .…”

“…PCR products were detected on 1% agarose gels, and those with specificity were separated by capillary electrophoresis using an ABI 3730xlDNAAnalyzer (Applied Biosystems, Foster City, CA, USA) 12 , the reagents were as follows: Applied Biosystems POP-7 Polymer and Hi-Di Formamide, running parameters: Run Voltage: 15,000, Injection Voltage: 1600, Injection Duration: 15, Temperature: 63, Laser Power: 12. Fluorescently labeled fragments were detected and sized using GeneMarker version 2.6.4 (Applied Biosystems, USA).…”

Development of Novel SNP Assays for Genetic Analysis of Rare Minnow (Gobiocypris rarus) in a Successive Generation Closed Colony

“…According to the classic definitions of laboratory animals' genetic background, the maintenance of limited genetic diversity is an essential requirement of a closed colony [40]. Heterozygosity has been introduced to genetic diversity research as a core indicator of genetic diversity [34,41,42]. The value of heterozygosity exhibits differences due to the use of different methods, such as genetic analysis using SNPs and SSRs between wild and seven cultured samples of pacific oyster (Crassostrea gigas) demonstrated lower mean heterozygosity using SNPs than with SSRs [43].…”

“…As heterozygosity may be an excellent indicator of genetic variability evaluation in a closed colony, it might be challenging to reach an agreement about the criterion of heterozygosity among different methods or species. For instance, in genetic variability testing of laboratory animals, based on SSR markers, the threshold of heterozygosity is generally recognized to be between 0.5 and 0.7 [41]. However, the criterion of heterozygosity in SSR analysis may not apply to the SNP method.…”