Development of an effective and efficient DNA isolation method for Cinnamomum species

Bs, Bhau; Gogoi, Gayatri; Baruah, Debanga Jyoti; Ahmed, Riyazuddin; Hazarika, G. C.; Ghosh, Swapankumar; Borah, Bibek Jyoti; Gogoi, Barnali; Dk, Sarmah; Sc, Nath; Sb, Wann

doi:10.1016/j.foodchem.2015.05.004

Cited by 16 publications

(7 citation statements)

References 18 publications

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“…Addition of 0.7% activated charcoal in extraction buffer helps to bind with resinous materials and negates the possibility of coextraction of impurities. Furthermore, its role in absorbing colored impurities and removing polyphenols also supports our method as previously reported by Bhau et al, (2015). (1996) Figure 1 PCR amplification of five microsatellites in G. soja.…”

Section: Resultssupporting

confidence: 87%

“…It has been reported that 2% CTAB concentration in extraction buffer improves cell membrane disruption (Doyle & Doyle, 1987;Bhau, et al, 2015). We employed 6 different concentrations of CTAB (1.5, 2.0, 2.5, 3.0, 3.5 and 4.0%).…”

Section: Resultsmentioning

confidence: 99%

“…Considering role of PVP in removing contamination during DNA extraction (Malencic et al, 2012;Dolatabadi et al, 2010;Bressan et al, 2014) four PVP concentrations were used (1, 2, 3 and 4%). Many researchers examined the effect of PVP on the quality and quantity of DNA in plants with higher levels of polyphenols and antioxidants (BHAU et al, 2015;Kit & Chandran 2013). We witnessed highest quantity of DNA (4979±1098 ng/µL with A260/A280 ratio1.89 ±0.7) with the extraction buffer having 3% PVP.…”

Section: Resultsmentioning

confidence: 99%

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Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species

Nawaz

Baloch

Rehman

et al. 2016

Turkish JAF Sci.Tech.

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Extraction of deoxyribose nucleic acid (DNA) from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.

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Section: Resultssupporting

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species

Nawaz

Baloch

Rehman

et al. 2016

Turkish JAF Sci.Tech.

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“…Salt concentration is critical for quality DNA extraction especially from plant materials [3][4][5][6][7]10,[15][16][17][18]. In fact, there are many different protocols and kits considering the importance salt concentration which have been used for DNA extraction from different plant tissues including banana [3][4][5][6][7]10,16,17,19,20]. We report for the first time a protocol with an optimal salt concentration (NaCl, 4mM) for quality DNA yield from banana fruits.…”

Section: Discussionmentioning

confidence: 99%

“…These biomolecule can be isolated from any biological material for subsequent downstream processes, analytical, or preparative purposes [1,2]. There are many different protocols and, kits commercially available for DNA extraction from different plant materials [2][3][4][5][6][7][8][9][10]. Varying amounts and integrity of DNA are obtained due to the inherent properties as it were, and, differences in the optimization of the protocols for DNA extraction [11,12].…”

Section: Introductionmentioning

confidence: 99%

Protocol Optimization of DNA Extraction from Banana Fruits

Omara

Barugahare²

2022

JABB

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Background: DNA extraction is the process by which the DNA is extracted and purified from the living or dead cells or tissues. There are many different kits commercially available for DNA extraction, with varying protocols. This study aimed at determining the optimal conditions for the extraction of high yield DNA from banana fruits. Methodology: DNA was extracted from banana fruits by precipitation method. The DNA yield was determined using the Nanodrop DNA quantification technique. Results: The optimal sodium chloride salt concentration for DNA extraction from banana fruits was 4mM and the amount of DNA extracted increased as the temperature decreased. The highest yield was obtained between 4 0C to 0 0C. Conclusion: We report for the first time a protocol with an optimal salt concentration (NaCl, 4mM) for quality DNA yield from banana fruits. A critical consideration is required to establish an optimal range of moderate temperatures and DNA concentrations for better quality and desired yields. There may not be a universal protocol for DNA extraction from all plant materials.

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Mass ratio quantitative detection for kidney bean in lotus seed paste using duplex droplet digital PCR and chip digital PCR

et al. 2020

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Development of an effective and efficient DNA isolation method for Cinnamomum species

Cited by 16 publications

References 18 publications

Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species

Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species

Protocol Optimization of DNA Extraction from Banana Fruits

Mass ratio quantitative detection for kidney bean in lotus seed paste using duplex droplet digital PCR and chip digital PCR

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