This study was conducted to estimate the relevance between the factors relating to the practical activities for infection prevention and control, performed by certified nurses in infection control (CNICs). A questionnaire survey was conducted to a total of 2,278 CNICs authorized by the Japanese Nursing Association. The responses were obtained from 708 subjects, and 698 of those were used to analyze as a valid response. Three factors were related to the activities: "holding an administrative position," "working as a full-time CNIC," and "participating in academic meeting or research seminar." In addition, "working in facilities receiving additional reimbursement for infection prevention of medical treatment fee" was also another relating factor. The frequency of practice relevant to "surveillance for healthcare-associated infection" and "occupational infection control" was increased especially in the facilities receiving Type I of additional reimbursement. Working in the facilities with less than 400 hospital beds or staffed by a single CNIC was thought to be a factor relating to the practical activities relevant to "infection control skills."