Development of an Eastern Blotting Technique for the Visual Detection of Aristolochic Acids in <i>Aristolochia</i> and <i>Asarum</i> Species by Using a Monoclonal Antibody Against Aristolochic Acids I and II

Li, Xiaowei; Morinaga, Osamu; Tian, Min; Uto, Takuhiro; Yu, Jie; Shang, Ming‐Ying; Wang, Xuan; Cai, Shao‐Qing; Shoyama, Yukihiro

doi:10.1002/pca.2448

Cited by 30 publications

(17 citation statements)

References 39 publications

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“…Because AA is a component of various Traditional Chinese Medicinal plants (7,(52)(53)(54)(55)(56), it is important to discover means of preventing AA-induced renal damage. Though studies have developed a monoclonal antibody against AA-induced cytotoxicity (3,57,58), most approaches are directed against oxidative stress to suppress AA-induced cytotoxicity. Based on the above findings, elucidating whether vitamin C inhibits AA-induced cytotoxicity in animal models will require further study.…”

Section: Discussionmentioning

confidence: 99%

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Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress-mediated cell death pathways

Wei

Pan

et al. 2015

Molecular Medicine Reports

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Aristolochic acid (AA) is a component of Chinese medicinal herbs, including asarum and aristolochia and has been used in Traditional Chinese Medicine for a long time. Recent studies found that AA has a cytotoxic effect resulting in nephropathy. These studies indicated that AA‑induced cytotoxicity is associated with increases in oxidative stress and caspase‑3 activation. The present study further demonstrated that AA mainly elevates the H2O2 ratio, leading to increases in oxidative stress. Furthermore, the results indicated that AA induces cell death can via caspase‑dependent and ‑independent pathways. It is desirable to identify means of inhibiting AA‑induced renal damage; therefore, the present study applied an anti‑oxidative nutrient, vitamin C, to test whether it can be employed to reduce AA‑induced cell cytotoxicity. The results showed that vitamin C decreased AA‑induced H2O2 levels, caspase‑3 activity and cytotoxicity in renal tubular cells. In conclusion, the present study was the first to demonstrate that AA‑induced increases of the H2O2 ratio resulted in renal tubular cell death via caspase‑dependent and ‑independent pathways, and that vitamin C can decrease AA‑induced increases in H2O2 levels and caspase‑3 activity to attenuate AA‑induced cell cytotoxicity.

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Section: Discussionmentioning

confidence: 99%

“…Aristolochic acid (AA) is contained in the Chinese medicinal herbs asarum and aristolochia (1)(2)(3). Numerous studies have demonstrated that AA can cause nephropathy (4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress-mediated cell death pathways

Wei

Pan

et al. 2015

Molecular Medicine Reports

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“…This group of plants has received a modest amount of attention due to their taxonomic difficulty (Blomquist, 1957; Gaddy, 1987; Yamaji et al, 2006, 2007; Weakley, 2012), broad disjunctions between morphologically similar species (Xiang and Soltis, 2001; Xiang et al, 2004), and medicinal and horticultural value (Han et al, 2008; Nivot et al, 2008; Quang et al, 2012; Kopyt'ko et al, 2013; Ku et al, 2013a, b; C. Li et al, 2013; X.‐W. Li et al, 2013; Perumalsamy et al, 2013; Williamson et al, 2013).…”

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confidence: 99%

Phylogenetic relationships in Asarum: Effect of data partitioning and a revised classification

Sinn

Kelly

Freudenstein

2015

American J of Botany

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Our analyses do not unambiguously support a single origin for all fungal-mimicry characters. Topologies recovered through the analysis of PartitionFinder-optimized matrices can differ drastically from those inferred from a priori partitioned matrices, and by analytical method. We recommend that investigators evaluate the topological effects of matrix partitioning using multiple methods of phylogenetic reconstruction.

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“…For those benefits, ELISA using anti-AAs polyclonal antibody (PAb) [58] and anti-AA-I and AA-IVa MAb [59] have been reported previously. We also prepared anti-AA-II MAb [60] and anti-AA-I and -II MAbs [61], and then investigated the localization of aristolochic acids in mice kidney [62] resulting in finding of α-actinin-4 as a possible target protein for aristolochic acid I in human kidney cell [63]. However, no immunostaining technique utilizing anti-AA MAb or anti-AA PAb has succeeded yet, due to the fact that AAs possess no capacity of conjugation with membrane and their aqueous solubility is quite poor.…”

Section: Aristolochia Speciesmentioning

confidence: 99%

Quality Control of Natural Products by Fingerprinting of Eastern Blotting

Fujii¹,

Tung²,

Uto³

et al. 2016

Pharm Anal Acta

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Eastern blotting was developed for small molecule compounds which have no affinity of conjugation to membrane instead of western blotting. Crude extracts of medicinal plants were developed by TLC plate followed to be blotted to polyvinylidene fluoride or polyether sulfone membrane by heating. Components on membrane were conjugated with carrier protein resulting in fixing to membrane. Staining was followed to western blotting system using monoclonal antibody (MAb). Four types of natural products, solasodine glycosides, aristrochic acid, glycyrrhizin-liquiritin and ginsenosides were discussed in this review. 1) Solasodine glycosides are selected as an example of staining for almost all solasodine glycosides using the anti-solamargine MAb having wide cross-reactivity. 2) Aristrochic acids possess no capacity of conjugation with membrane and their aqueous solubility is quite poor. To improving them, aristrochic acids should be synthesized for conjugation with carrier protein in order to combine to membrane. 3) Two different types of marker components in licorice, triterpene glycoside; glycyrrhizin and flavonoid glycoside; liquiritin can be detected by double eastern blotting. 4) Ginsenosides are also detected by double eastern blotting and this system might be possible to clear the distribution of ginsenosides in tissue for understanding of pharmacological activity.

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Development of an Eastern Blotting Technique for the Visual Detection of Aristolochic Acids in Aristolochia and Asarum Species by Using a Monoclonal Antibody Against Aristolochic Acids I and II

Abstract: This method was extremely useful for the visual screening of AA-I and AA-II among easily mistaken herbal medicines.

Cited by 30 publications

References 39 publications

Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress-mediated cell death pathways

Vitamin C attenuates the toxic effect of aristolochic acid on renal tubular cells via decreasing oxidative stress-mediated cell death pathways

Phylogenetic relationships in Asarum: Effect of data partitioning and a revised classification

Quality Control of Natural Products by Fingerprinting of Eastern Blotting

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