Development of an automatic machine for in Situ hybridization and immunohistochemistry

Takahashi, T; Ishiguro, Katsuyuki

doi:10.1016/0003-2697(91)90484-b

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…BrdU, GFAP, and NeuN expression were measured by immunofluorescent staining, araffin followed by embedding (Takahashi and Ishiguro, 1991). Coronal sections (5 µm) were continuously taken by paraffin slicing machine (HM 340E Rotary Mi- crotome, Thermo Scientific Co,.Ltd) with one slice chosen from every six slices.…”

Section: Immunofluorescent Stainingmentioning

confidence: 99%

“…Coronal sections (5 µm) were continuously taken by paraffin slicing machine (HM 340E Rotary Mi- crotome, Thermo Scientific Co,.Ltd) with one slice chosen from every six slices. After dewaxing (Takahashi and Ishiguro, 1991), antigens were retrieved in 0.01 mol/L sodium citrate buffer (pH = 6.0) and soaked in PBS containing 3% H 2 O 2 to suppress the activity of endogenous peroxidase. Following incubation in 2 mol/L HCl at 37 • C for 50 minutes, BrdU antigens of DNA were unmasked.…”

Section: Immunofluorescent Stainingmentioning

confidence: 99%

“…BDNF and TrkB protein expression was measured by immunohistochemical test. Methods for material collection, paraffin embedding, slicing, dewaxing (Takahashi and Ishiguro, 1991), highpressure antigen retrieval and suppression of endogenous peroxidase activity were the same as for immunofluorescence staining. Either anti-mouse BDNF (1:200) or TrkB (1:200) primary antibody was added and the solution cultured overnight (4 • C).…”

Section: Immunohistochemical Testmentioning

confidence: 99%

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Promoting neurogenesis in hippocampal dentate gyrus of chronic unpredictable stress-induced depressive-like rats with paeoniflorin

Chen

Qiu

Zhang

et al. 2019

J. Integr. Neurosci.

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J o u r n a l o f I n t e g r a t i v e N e u r o s c i e n c eThis is an open access article under the CC BY-NC 4.0 license (https://creativecommons.org/licenses/by-nc/4.0/) Hippocampal neurogenesis plays an important role in the onset and treatment of depressive disorders. Previous studies suggest that paeoniflorin could be used as an antidepressant for treating rats subjected to chronic unpredictable stress. In this study, the effects of paeoniflorin on neurogenesis in the hippocampus dentate gyrus and potential mechanism of action are further investigated in chronic unpredictable stress-induced rat. Results suggest that paeoniflorin markedly increased both sucrose consumption and the number of 5-bromo-2-deoxyuridinepositive cells in the dentate gyrus of chronic unpredictable stress-induced rats, and the ratio of co-expressed 5-bromo-2-deoxyuridine and glial fibrillary acidic protein-positive cells, but exerted no significant effect on the ratio of coexpressed 5-bromo-2-deoxyuridine and neuronal nucleipositive cells. Compared with the vehicle group, a significant increase was detected in the number of brainderived neurotrophic factor-positive cells and the expression of brain-derived neurotrophic factor mRNA in the hippocampus of the paeoniflorin-treated group. According to the results, paeoniflorin promoted neural stem cell proliferation, their differentiation into astrocytes, and neurogenesis in the hippocampal dentate gyrus of chronic unpredictable stress-induced rats. Apart from enhancing the protein expression and gene transcription of brainderived neurotrophic factor, it also activated the expression of tropomyosin receptor kinase B (a high-affinity receptor of brain-derived neurotrophic factor). This suggests that paeoniflorin might promote neurogenesis in the hippocampus dentate gyrus of chronic unpredictable stressinduced rats and act as an antidepressant by regulating the brain-derived neurotrophic factor-tropomyosin receptor kinase B signaling pathway.

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Section: Immunofluorescent Stainingmentioning

confidence: 99%

Section: Immunofluorescent Stainingmentioning

confidence: 99%

Section: Immunohistochemical Testmentioning

confidence: 99%

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