Development of an automated microbial sensor system1This paper was presented at the Fifth World Congress on Biosensors, Berlin, Germany, 3–5 June 1998.1

Heim, Sabina; Schnieder, Inga; Binz, D; Vogel, Alexandra; Bilitewski, Ursula

doi:10.1016/s0956-5663(98)00118-3

Cited by 44 publications

(9 citation statements)

References 4 publications

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“…A schematic drawing of the cell construction according to a reported specification [26,39] is given in Fig. 2.…”

Section: Flow-through Cell Constructionmentioning

confidence: 99%

“…The composition of pastes and details of their fabrication procedure influence the electrochemical performance of the resulting electrode and have to be adapted with respect to the intended application. Platinum pastes are usually used for the fabrication of working electrodes used for the oxidation of hydrogen peroxide [21,22,23,24,25] or the reduction of oxygen [26], as the potential required for oxidation of hydrogen peroxide at graphite composites is rather high [27]. Carbonaceous pastes or inks are used when the enzyme or antibody is entrapped within the paste [17,28,29], direct electron transfer between enzyme and electrode is used [30,31] or the detection is based on compounds showing favorable electrochemistry at carbon electrodes.…”

Section: Introductionmentioning

confidence: 99%

“…Isolating layers on top of an electrically conducting layer define the electrode area and the volume that can be placed on top of the electrode. Thus, a thin layer flow-through cell with an inner volume of 36 µL fabricated totally by screen-printing technology has been described [26,39] avoiding the necessity for specially designed and fabricated flow cells, in which a screen-printed electrode is placed. For batch analysis even smaller vials have been fabricated.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, a flow-through cell comparable to the one described in [39] and by Heim et al. [26] should be used not only as an integrated electrochemical detector but also as an affinity reactor to open the possibilities of a common technology for the fabrication of complex analytical systems. The principle of the assay was based on earlier work combining a homogeneous immunoreaction with the capture of the immunocomplexes through the high-affinity biotin-streptavidin-system [16].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical characterization of screen-printed carbonaceous electrodes for the determination of peroxidase activity in novel screen-printed flow-through modules

Stiene

Bilitewski

2002

Analytical and Bioanalytical Chemistry

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A novel totally screen-printed flow-through cell for immunoanalysis is presented. It contained screen-printed carbonaceous electrodes, which allowed the determination of peroxidase activity through the electrochemical reduction of p-benzoquinone. As different electrode materials differ strongly in their electrochemical properties, electrodes resulting from various screen-printable carbonaceous pastes were characterized using the hydroquinone/ p-benzoquinone redox couple. For most of the electrodes, cyclic voltammogram peak separations of between 550 and 670 mV were observed indicating only quasi-reversible electrochemical behavior. This was confirmed by variation of the peak separation with scan rate. Heterogeneous electron transfer rates of ca. 0.5 - 1 x 10(-3) cm s(-1) and electrochemical activation energies of ca. 20 kJ mol(-1) were found. These flow-through cells were not only applied to electrochemical peroxidase activity determinations but also, in combination with a separate detector, as affinity reactors. After biotinylation of screen-printed layers, streptavidin and then biotinylated peroxidase could be bound. However, as signals were only 10-20% of those obtained with a column filled with biotinylated glass beads, only the screen-printed electrochemical detector was applied to the detection of antibodies against the African Swine Fever Virus.

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“…A schematic drawing of the cell construction according to a reported specification [26,39] is given in Fig. 2.…”

Section: Flow-through Cell Constructionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrochemical characterization of screen-printed carbonaceous electrodes for the determination of peroxidase activity in novel screen-printed flow-through modules

Stiene

Bilitewski

2002

Analytical and Bioanalytical Chemistry

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“…Additionally, miniaturization allows the development of portable analytical systems to be used for in‐field applications . This kind of miniaturized electrodes can be fabricated using microfabrication or screen‐printing technologies, which has been widely used for making micro‐electrodes for different biosensors and biosensor arrays .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Characterization of the Microfabricated Electrochemical Sensor‐Array System

et al. 2016

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Microfabrication technology has been used to prepare a microchip sensor‐array with six sets of platinum electrodes. Chromium/platinum (10 nm/100 nm thick) were sputtered on a borosilicate wafer and patterned by wet etching method. The electrodes were designed with working electrode area of 700×400 μm in the middle and a 200 μm wide and 2600 μm long counter electrode surrounding it from three sides in a U‐shape. The connection pads (1000×1500 μm) were located at the edge of a sensor‐array chip. Silicon wafer was etched through to form holes with slanting side walls for immobilization cavities. The silicon and the borosilicate wafers were adhesion bonded with SU‐8 epoxy resin. The cyclic voltammetry and electrochemical impedance experiments were carried out in a three‐electrode electrochemical system to characterize the fabricated sensor‐array chip. The results show that the current density depends on the electrode potential sweep rate ν. Also, current density depends on the concentration of potassium hexacyanoferrate(III). At slow potential sweep rates (ν≤0.01 V s−1) the steady‐state signal is achieved and the electrodes behave as micro‐electrodes. Such an array is a promising candidate for fast and simple biochemical oxygen demand (BOD) measurements.

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A novel microbial BOD biosensor developed by the immobilization of P. Syringae in micro‐cellular polymers

Kara

Keskinler

Erhan

2008

J of Chemical Tech & Biotech

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BACKGROUND: A biochemical oxygen demand (BOD) sensor, based on an immobilized Pseudomonas syringae in highly porous micro-cellular polymer (MCP) in combination with a dissolved oxygen electrode, has been developed for the analysis of biodegradable organic compounds in aqueous samples. Microorganisms were immobilized in a molded MCP disk and a wastewater sample was injected into the biocomposite disk by a flow injection system. Dissolved oxygen (DO) changes as a measure of soluble BOD was read with a DO probe placed into a flow cell carrying biocatalytically activated disk.

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Development of an automated microbial sensor system1This paper was presented at the Fifth World Congress on Biosensors, Berlin, Germany, 3–5 June 1998.1

Cited by 44 publications

References 4 publications

Electrochemical characterization of screen-printed carbonaceous electrodes for the determination of peroxidase activity in novel screen-printed flow-through modules

Electrochemical characterization of screen-printed carbonaceous electrodes for the determination of peroxidase activity in novel screen-printed flow-through modules

Electrochemical Characterization of the Microfabricated Electrochemical Sensor‐Array System

A novel microbial BOD biosensor developed by the immobilization of P. Syringae in micro‐cellular polymers

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