Development of an athyroid mouse model using 131I ablation after preparation with a low-iodine diet

Oh, Ji Min; Lee, Ho Won; Kalimuthu, S; Gangadaran, Prakash; Baek, Se Hwan; Han, Man‐Hoon; Hong, Chae Moon; Jeong, Shin Young; Lee, Sang‐Woo; Lee, Jaetae; Ahn, Byeong‐Cheol

doi:10.1038/s41598-017-13772-8

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…In this work, we present a synchrotron-based measurement of the endogenous thyroidal iodine mass in wildtype and Rag1-deficient C57BL/6 mice using x-ray fluorescence imaging to demonstrate the detection of small quantities of iodine. Previous studies based on single photon emission computed tomography (SPECT) and scintigraphy rely on the use of radiotracers—typically between 10 and 300 µCi (0.4 to 11 MBq) 125 I or 131 I 20 – 24 or even exceeding 800 µCi (30 MBq) 25 and have only been able to detect uptake rates of iodine radiotracer 26 , 27 . Local radiation dose in rats were determined to be 2.6 Gy/MBq for 125 I and 21 Gy/MBq for 131 I and doses in mice are expected to be even higher due to the smaller volume 22 , 28 .…”

Section: Introductionmentioning

confidence: 99%

In-situ x-ray fluorescence imaging of the endogenous iodine distribution in murine thyroids

Körnig

Staufer

Schmutzler

et al. 2022

Sci Rep

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X-ray fluorescence imaging (XFI) is a non-invasive detection method of small quantities of elements, which can be excited to emit fluorescence x-ray photons upon irradiation with an incident x-ray beam. In particular, it can be used to measure nanoparticle uptake in cells and tissue, thus making it a versatile medical imaging modality. However, due to substantially increased multiple Compton scattering background in the measured x-ray spectra, its sensitivity severely decreases for thicker objects, so far limiting its applicability for tracking very small quantities under in-vivo conditions. Reducing the detection limit would enable the ability to track labeled cells, promising new insights into immune response and pharmacokinetics. We present a synchrotron-based approach for reducing the minimal detectable marker concentration by demonstrating the feasibility of XFI for measuring the yet inaccessible distribution of the endogenous iodine in murine thyroids under in-vivo conform conditions. This result can be used as a reference case for the design of future preclinical XFI applications as mentioned above.

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Section: Introductionmentioning

confidence: 99%

In-situ x-ray fluorescence imaging of the endogenous iodine distribution in murine thyroids

Körnig

Staufer

Schmutzler

et al. 2022

Sci Rep

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“…As an example, it may be mentioned that the effectiveness of radioiodine therapy in metastases generally produces excellent prognostics when they are avid for iodine (Barbolosi et al 2017). In this regard, in many situations, it is common and indicated to administer drugs to alter hormone levels in patients before treatments, in order to optimize responses to therapies (Bonnema and Hegedüs 2012;Oh et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Mathematical models applied to thyroid cancer

et al. 2019

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Thyroid cancer is the most prevalent endocrine neoplasia in the world. The use of mathematical models on the development of tumors has yielded numerous results in this field and modeling with differential equations is present in many papers on cancer. In order to know the use of mathematical models with differential equations or similar in the study of thyroid cancer, studies since 2006 to date was reviewed. Systems with ordinary or partial differential equations were the means most frequently adopted by the authors. The models deal with tumor growth, effective half-life of radioiodine applied after thyroidectomy, the treatment with iodine-131, thyroid volume before thyroidectomy, and others. The variables usually employed in the models includes tumor volume, thyroid volume, amount of iodine, thyroglobulin and thyroxine hormone, radioiodine activity, and physical characteristics such as pressure, density, and displacement of the thyroid molecules. In conclusion, the mathematical models used so far with differential equations approach several aspects of thyroid cancer, including participation in methods of execution or follow-up of treatments. With the development of new models, an increase in the current understanding of the detection, evolution, and treatment of diseases is a step that should be considered.

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“…One hour later, the mice were sacrificed after drawing blood samples from all mice. The uptake values were measured in the following organs: lungs, heart, liver, stomach, spleen, intestine, kidney, muscle, and thyroid using a gamma-counter (Cobra II) ( Oh et al, 2017 ). The data were expressed as the injected dose per gram tissue in percentage (%ID/g).…”

Section: Methodsmentioning

confidence: 99%

In vivo Non-invasive Imaging of Radio-Labeled Exosome-Mimetics Derived From Red Blood Cells in Mice

Gangadaran

Hong

et al. 2018

Front. Pharmacol.

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Exosomes are natural nano-sized membrane vesicles that have garnered recent interest owing to their potential as drug delivery vehicles. Though exosomes are effective drug carriers, their production and in vivo biodistribution are still not completely elucidated. We analyzed the production of exosome mimetics (EMs) from red blood cells (RBCs) and the radio-labeling of the RBC-EMs for in vivo imaging. Engineered EMs from RBCs were produced in large-scale by a one-step extrusion method, and further purified by density-gradient centrifugation. RBC-EMs were labeled with technetium-99m (99mTc). For non-invasive imaging, 99mTc (free) or 99mTc-RBC-EMs were injected in mice, and their biodistribution was analyzed by gamma camera imaging. Animals were sacrificed, and organs were collected for further biodistribution analysis. RBC-EMs have similar characteristics as the RBC exosomes but have a 130-fold higher production yield in terms of particle numbers. Radiochemical purity of 99mTc-RBC-EMs was almost 100% till 2 h reduced to 97% at 3 h. Radio-labeling did not affect the size and morphology of RBC-EMs. In contrast to free 99mTc, in vivo imaging of 99mTc-RBC-EMs in mice showed higher uptake in the liver and spleen, and no uptake in the thyroid. Ex vivo imaging confirmed the in vivo findings. Furthermore, fluorescent imaging confirmed the nuclear imaging findings. Immunofluorescent imaging revealed that the hepatic uptake of RBC-EMs was significantly mediated by kupffer cells (resident hepatic macrophages). Our results demonstrate a simple yet large-scale production method for a novel type of RBC-EMs, which can be effectively labeled with 99mTc, and feasibly monitored in vivo by nuclear imaging. The RBC-EMs may be used as in vivo drug delivery vehicles.

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Development of an athyroid mouse model using 131I ablation after preparation with a low-iodine diet

Cited by 4 publications

References 32 publications

In-situ x-ray fluorescence imaging of the endogenous iodine distribution in murine thyroids

In-situ x-ray fluorescence imaging of the endogenous iodine distribution in murine thyroids

Mathematical models applied to thyroid cancer

In vivo Non-invasive Imaging of Radio-Labeled Exosome-Mimetics Derived From Red Blood Cells in Mice

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