Development of an assay of seven biochemical items, HbA1c, and hematocrit using a small amount of blood collected from the fingertip

Sugimoto, Shinya; Akimoto, Masaru; Hayakawa, Akira; Hokazono, Eisaku; Osawa, Satoshi

doi:10.1016/j.cca.2011.09.021

Cited by 11 publications

(10 citation statements)

References 8 publications

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“…Therefore, the metabolic status, including the levels of blood sugar, triglycerides, cholesterols and TNF‐ α , of the subjects could not be determined. Although we showed the link between body fat composition and the level of TNF‐ α on the skin, it would also be interesting to investigate the link between such metabolic parameters and skin inflammation by utilizing a fingertip blood test kit .…”

Section: Discussionmentioning

confidence: 95%

Increased level of tumour necrosis factor‐alpha (TNF‐α) on the skin of Japanese obese males: measured by quantitative skin blotting

Ogai

Matsumoto

Aoki

et al. 2016

Intern J of Cosmetic Sci

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OBJECTIVE A state of chronic inflammation, characterized by an increased level of tumor necrosis factor-alpha (TNF-α), is often found in the obese population. The negative effects of elevated TNF-α are not limited to systemic metabolism. It also reportedly affects skin integrity. Recently, the relationship between obesity and skin fragility was reported; however, there has been little insight into how the level of TNF-α in the skin in situ is related to the severity of obesity. In this study, we aimed to measure the level of TNF-α on the skin and to find the relationship between obesity and the level of TNF-α detected on the skin. METHODSWe used a novel, non-invasive method called quantitative skin blotting.Fifty-nine healthy (but some were classified as being overweight or obese) Japanese males were enrolled as subjects. The levels of TNF-α detected on the abdominal and thigh skin along with the body composition were measured, followed by a correlation analysis. RESULTSSignificant positive correlations were found between the levels of TNF-α detected on the skin and the severity of obesity such as body mass index (BMI), body fat weight, and visceral fat rating. CONCLUSIONWe found that high levels of TNF-α were detected on the skin of Japanese obese males, which implied the higher TNF-α in the skin. The elevation of skin TNF-α may be one factor related to skin fragility that is often found in obese individuals.

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Section: Discussionmentioning

confidence: 95%

Increased level of tumour necrosis factor‐alpha (TNF‐α) on the skin of Japanese obese males: measured by quantitative skin blotting

Ogai

Matsumoto

Aoki

et al. 2016

Intern J of Cosmetic Sci

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“…Notably, a population of medium-sized morphologically atypical lymphocytes in the blood accounted for 9% of all lymphocytes. The concentrations of liver enzymes were slightly increased compared with normal ranges; the concentration of alanine aminotransferase was 52 U/l (normal, 9-50 U/l) (11) and of aspartate aminotransferase was 30 U/l (normal, 15-40 U/l) (11). The level of triglyceride was normal.…”

Section: Case Reportmentioning

confidence: 94%

Hemophagocytic lymphohistiocytosis presenting with annular erythema multiforme‑like eruptions in a patient with angioimmunoblastic T cell lymphoma: A case report

et al. 2018

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Angioimmunoblastic T cell lymphoma (AITL)-associated hemophagocytic lymphohistiocytosis (HLH) rarely occurs with annular erythema multiforme-like rashes. The present case report describes a patient who was misdiagnosed with erythema multiforme at an early stage of the disease due to annular erythema multiforme-like eruptions. However, antihistamine treatment was ineffective. The patient progressed rapidly with high fever, hepatosplenomegaly and pharyngitis. The number of copies of Epstein-Barr virus DNA continuously increased. Accompanied by the swelling of lymph nodes, the blood cell count decreased. Further bone-marrow examination and biopsy of the lymph nodes were conducted. The patient was eventually diagnosed with AITL-associated HLH, and treated with etoposide together with cyclophosphamide, doxorubicin, vincristine and prednisolone. The patient was successfully treated with several courses of chemotherapy. In view of the fact that AITL-associated HLH with annular erythema multiforme-like rashes is relatively rare worldwide and is associated with a high mortality rate, the data on previous cases were reviewed with the hope of providing clinical bases for early diagnosis and treatment of AITL-associated HLH.

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“…The dilution ratio of the plasma released into the diluted solution is measured post hoc using the difference in absorbance between the internal standard materials contained in the hyperosmotic buffer. 3,7 However, this method may cause a change in the plasma components, because it is inevitable that water and low-molecular-weight substances in blood cells will diffuse into the diluted solution due to the hyperosmotic buffer used to avoid hemolysis during pressure application in the membrane separation process. Another problem with this method is that measurement variations, even in the common range of 2% to 3%, may greatly affect the calculated dilution ratio because of very small differences in the absorbance of the internal standard materials used to determine the plasma dilution ratio…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3][4][5] For example, correlations between assay results using blood samples collected from a vein, such as the ante-cubital vein (hereinafter referred to as "venous blood"), and fingertip blood samples have been reported for biochemical tests. 4 In addition, it has been suggested that hematological tests can be also measured in fingertip blood samples as long as test values are within the reference intervals established by the Japanese Committee for Clinical Laboratory Standards and if the following two conditions are met: capillary-collected blood is promptly transferred into a microliter-scale blood collection tube containing ethylenediaminetetraacetic acid (EDTA) and contamination with interstitial fluid by forced blood extrusion is avoided.…”

mentioning

confidence: 99%

Correlation of the Blood Test Results Obtained between Assays Using Microliter-scale Fingertip Blood Samples Collected with a Novel Blood Collection Device and Conventional Venous Blood Assays: a Secondary Publication in English

Iwasawa¹,

Nishimura²,

Nemoto³

et al. 2017

Keio J. Med.

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In this study, we investigated the correlations between biochemical and hematological test results obtained using microliter-scale fingertip blood samples collected with a newly developed blood collection device and those obtained using conventional venous blood. Eighty volunteer subjects were enrolled in this study. Blood samples were drawn from the fingertip of the ring finger by a single puncture, and 60-µL samples were promptly and accurately aspirated into a blood collection chip. Then the chip was tightly sealed in a chip container and was shaken to mix the contents without dispersion. For biochemical tests other than that for HbA1c, blood was collected without anticoagulant and centrifuged to obtain 15 µL of serum which was then diluted with 190 µL of physiological saline for the assay. For hematological tests and the test for HbA1c, the sample was assayed with blood collected using EDTA-2 K. Good correlations were obtained between the test results of the assay using fingertip blood and that using venous blood. The correlation coefficients were ≥0.97 for TG, T-CHO, HDL-C, LDL-C, GLU, ALT, γ-GTP, UA, BUN, and HbA1c and ≥0.95 for WBC, RBC, Hgb, and Hct. These results suggest that our microliter-scale blood testing system is comparable to assays using venous blood and may be useful as a rapid and simple test to determine basic clinical parameters that are close to the reference intervals.

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Development of an assay of seven biochemical items, HbA1c, and hematocrit using a small amount of blood collected from the fingertip

Cited by 11 publications

References 8 publications

Increased level of tumour necrosis factor‐alpha (TNF‐α) on the skin of Japanese obese males: measured by quantitative skin blotting

Increased level of tumour necrosis factor‐alpha (TNF‐α) on the skin of Japanese obese males: measured by quantitative skin blotting

Hemophagocytic lymphohistiocytosis presenting with annular erythema multiforme‑like eruptions in a patient with angioimmunoblastic T cell lymphoma: A case report

Correlation of the Blood Test Results Obtained between Assays Using Microliter-scale Fingertip Blood Samples Collected with a Novel Blood Collection Device and Conventional Venous Blood Assays: a Secondary Publication in English

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